超声酶辅助海藻糖脂提取黄连生物碱的工艺优化及抗炎作用评价

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry Pub Date : 2025-05-23 DOI:10.1016/j.ultsonch.2025.107396

Huiwen Wang , Haixin Peng , Wenwen Ding , Zhiyun Zhang , Yadan Zheng , Sikai Chen , Xueying Chen , Qianwei Qu , Yanyan Liu , Yanhua Li

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引用次数: 0

摘要

黄连是一种富含异喹啉类生物碱的中药，具有广泛的药理活性。然而，这些生物碱的有限溶解度提出了一个重大挑战，阻碍了通过传统提取方法充分实现CR的治疗潜力。为了解决这一问题，提高目标化合物的溶解度，同时优化提取效率，本研究采用环境可持续的超声-酶-海藻糖脂（UET）提取技术，协同提取CR中5种关键生物碱，并在单因素实验结果的基础上，采用Box-Behnken设计（BBD）对所选模型变量进行优化。确定了最佳提取条件：提取pH为4.3，提取温度为40℃，料液比为1:27 g/mL，超声时间为48 min。与其他提取液进行对比，证明UET提取液具有较高的生物碱提取率、综合评价值（CEV）、较低的能耗和CO2排放。利用扫描电镜（SEM）、动态光散射（DLS）和透射电镜（TEM）对UET萃取机理进行了探讨。此外，UET提取物具有更优异的抗炎活性。UET方法的建立为绿色高效提取天然药物中的生物碱成分提供了方法参考。

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Optimization of ultrasound enzyme-assisted trehalose lipid extraction of Coptis alkaloids and evaluation of its anti-inflammatory effects

Coptidis Rhizoma (CR), a traditional Chinese medicine, has extensive pharmacological activity because it is rich in isoquinoline alkaloids. Nonetheless, the limited solubility of these alkaloids presents a significant challenge, hindering the full realization of CR’s therapeutic potential through conventional extraction methods. To address this issue and enhance the solubility of the target compounds while optimizing extraction efficiency, this study employed an environmentally sustainable extraction technique, Ultrasound-Enzyme-Trehalose Lipid (UET), to synergistically extract five key alkaloids from CR. Based on the results of single-factor experiments, Box-Behnken design (BBD) was employed to optimize the selected model variables. The optimal extraction conditions were determined: extraction pH of 4.3, extraction temperature of 40 ℃, solid–liquid ratio of 1:27 g/mL, and ultrasonication time of 48 min. UET extracts were compared with other extracts, and it was proved that UET extracts had higher alkaloid extraction rate, comprehensive evaluation value (CEV) and lower energy consumption and CO₂ emission. Then scanning electron microscopy (SEM), dynamic light scattering (DLS) and transmission electron microscopy (TEM) were used to explore the extraction mechanism of UET extraction. In addition, UET extraction has more excellent anti-inflammatory activity. The establishment of UET method of CR provides a method reference for green and efficient extraction of alkaloid components from natural drugs.

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来源期刊

Ultrasonics Sonochemistry 化学-化学综合

CiteScore

15.80

自引率

11.90%

发文量

361

审稿时长

59 days

期刊介绍： Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.