MicroRNA-34-5p调控蜱唾液腺变性过程中ECR的表达。

IF 3 2区 医学 Q1 PARASITOLOGY
Shanming Hu, Yanan Wang, Yongzhi Zhou, Jie Cao, Houshuang Zhang, Jinlin Zhou
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引用次数: 0

摘要

背景:雌性蜱的唾液腺在摄食后通过体外类固醇受体介导的程序性细胞死亡迅速退化。变性包括细胞凋亡和自噬。变性过程也可以通过microrna (miRNA)进行调节,但miRNA参与唾液腺变性的潜在机制尚不完全清楚。在这里,我们证明了microRNA34-5p (miR-34-5p)通过调节靶基因RhECR来调节血蚜根茎唾液腺变性的过程。方法:双荧光素酶报告基因测定和表型拯救实验确定RhECR是miR-34-5p的直接靶点。通过苏木精伊红(H&E)和末端脱氧核苷酸转移酶dUTP镍端标记(TUNEL)染色定量检测miR-34-5p的过表达和抑制。结果:结果显示miR-34-5p抑制RhECR的表达,延缓唾液腺腺泡细胞凋亡。该研究确定了miR-34-5p和RhECR在蜱唾液腺变性中的作用及其相互作用。结论:本研究结果将有助于ECR基因在蜱虫控制中的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MicroRNA-34-5p regulates the expression of ecdysteroid receptor (ECR) in the process of salivary gland degeneration of ticks.

Background: The salivary glands of female ticks rapidly degenerate after feeding via programmed cell death mediated by an ecdysteroid receptor (ECR). The degeneration includes both apoptosis and autophagy. The process of degeneration can also be regulated by microRNAs (miRNAs), but the underlying mechanism of miRNA involvement in salivary gland degeneration remains incompletely understood. Here, we demonstrate that microRNA34-5p (miR-34-5p) regulates the process of salivary gland degeneration in Rhipicephalus haemaphysaloides by modulating the target gene RhECR.

Methods: Dual luciferase reporter assays and phenotypic rescue experiments identified RhECR as a direct target of miR-34-5p. The overexpression and inhibition of miR-34-5p were quantified by hematoxylin and eosin (H&E) and Terminal deoxynucleotidyl transferase dUTP Nick-End Labeling (TUNEL) staining.

Results: The results showed that miR-34-5p inhibited the expression of RhECR to retard apoptosis in salivary gland acini. The study identified the roles of miR-34-5p and RhECR and their interactions in tick salivary gland degeneration.

Conclusions: The findings will aid in the application of ECR genes for tick control.

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来源期刊
Parasites & Vectors
Parasites & Vectors 医学-寄生虫学
CiteScore
6.30
自引率
9.40%
发文量
433
审稿时长
1.4 months
期刊介绍: Parasites & Vectors is an open access, peer-reviewed online journal dealing with the biology of parasites, parasitic diseases, intermediate hosts, vectors and vector-borne pathogens. Manuscripts published in this journal will be available to all worldwide, with no barriers to access, immediately following acceptance. However, authors retain the copyright of their material and may use it, or distribute it, as they wish. Manuscripts on all aspects of the basic and applied biology of parasites, intermediate hosts, vectors and vector-borne pathogens will be considered. In addition to the traditional and well-established areas of science in these fields, we also aim to provide a vehicle for publication of the rapidly developing resources and technology in parasite, intermediate host and vector genomics and their impacts on biological research. We are able to publish large datasets and extensive results, frequently associated with genomic and post-genomic technologies, which are not readily accommodated in traditional journals. Manuscripts addressing broader issues, for example economics, social sciences and global climate change in relation to parasites, vectors and disease control, are also welcomed.
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