在紧急手术病理患者和covid -19后综合征背景下，DAMP细胞毒性组分在免疫标志物破坏中的作用

IF 3.3 4区医学 Q3 IMMUNOLOGY

Immunology letters Pub Date : 2025-05-21 DOI:10.1016/j.imlet.2025.107033

Elena M. Klimova , Olena V. Lavinska , Larisa A. Drozdova

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引用次数: 0

摘要

背景：由于SARS-CoV-2大流行，形成了不同的急性和无症状COVID-19人群。在这些人群中进行的一项调查同样频繁地显示出虚弱的症状复合物，即所谓的covid -19后综合征（PCS）。在PCS和各器官的结构和功能障碍的背景下，紧急手术病理的频率增加。目的是研究在PCS背景下发生的紧急外科病理患者免疫抵抗因子的动态变化，并确定严重病程的病原标志物和死亡风险。材料与方法：采用荧光显微镜、共聚焦显微镜、流式细胞术、分光光度法、酶联免疫吸附试验（ELISA）对PCS合并急诊心血管（n = 103）和腹部（n = 106）病理的患者进行检查。结果：我们揭示了紧急外科病理和PCS合并症患者免疫功能障碍的时间依赖性。DAMP（损伤相关分子模式）细胞毒性组分比率的性质与先天和适应性免疫因子、病情严重程度和死亡风险的某些变化有关。在第一阶段（2020-2021年），PCS患者存在先天免疫的体液和细胞成分紊乱，背景是寡肽和多肽DAMP组分增加。在PCS发育的第二阶段（2022-2024），在细胞毒性寡核苷酸DAMP部分增加的背景下，观察到先天和适应性免疫的变化（死亡率为17.3%）。结论：心血管和腹部急症病理中免疫抵抗受损的标志物可用于选择靶向治疗策略。

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The role of DAMP cytotoxic fractions in the immune markers’ disruption in patients with urgent surgical pathology and against the background of post-COVID-19 syndrome

Background

As a result of the SARS-CoV-2 pandemic, various population groups were formed that had acute and asymptomatic COVID-19. A survey in these groups revealed with equal frequency an asthenic symptom complex, the so-called post-COVID-19 syndrome (PCS). The frequency of urgent surgical pathology against the background of PCS and structural and functional disorders of various organs was increased. The aim – to study the dynamics of immunoresistance factors changes in patients with urgent surgical pathology that developed against the background of PCS and to identify pathogenic markers of the severe course and the risk of mortality.

Materials and methods

To examine patients with PCS and urgent cardiovascular (n = 103) and abdominal (n = 106) pathology we used the following methods: fluorescence microscopy, confocal microscopy, flow cytometry, spectrophotometry, ELISA.

Results

We revealed a temporal dependence of immune dysfunction in patients with a comorbid course of urgent surgical pathology and PCS. The nature of the DAMP (damage-associated molecular patterns) cytotoxic fractions ratio was associated with certain changes in innate and adaptive immunity factors, severity of the condition and risk of mortality. At the first stage (2020–2021), patients with PCS has disorders of the humoral and cellular components of innate immunity against the background of an increase in the oligopeptide and peptide DAMP fractions. At the second stage (2022–2024) of PCS development, changes in innate as well as adaptive immunity were observed against the background of an increase in the cytotoxic oligonucleotide DAMP fraction (mortality was 17.3 %).

Conclusions

The identified markers of impaired immunoresistance in cardiovascular and abdominal urgent pathology can be used to select targeted therapy tactics.

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来源期刊

Immunology letters 医学-免疫学

CiteScore

7.60

自引率

0.00%

发文量

审稿时长

44 days

期刊介绍： Immunology Letters provides a vehicle for the speedy publication of experimental papers, (mini)Reviews and Letters to the Editor addressing all aspects of molecular and cellular immunology. The essential criteria for publication will be clarity, experimental soundness and novelty. Results contradictory to current accepted thinking or ideas divergent from actual dogmas will be considered for publication provided that they are based on solid experimental findings. Preference will be given to papers of immediate importance to other investigators, either by their experimental data, new ideas or new methodology. Scientific correspondence to the Editor-in-Chief related to the published papers may also be accepted provided that they are short and scientifically relevant to the papers mentioned, in order to provide a continuing forum for discussion.