槲皮素对实验性铜酮诱导的雄性C57BL/6小鼠多发性硬化的保护作用

IF 2.1 Q3 PHYSIOLOGY
Samin Ghasemi , Shahin Hassanpour , Razieh Hosseini
{"title":"槲皮素对实验性铜酮诱导的雄性C57BL/6小鼠多发性硬化的保护作用","authors":"Samin Ghasemi ,&nbsp;Shahin Hassanpour ,&nbsp;Razieh Hosseini","doi":"10.1016/j.crphys.2025.100146","DOIUrl":null,"url":null,"abstract":"<div><div>This study aimed to determine protective effect of the Quercetin on experimental cuprizone-induced (CPZ) multiple sclerosis (MS) in Male C57BL/6 mice. Forty male C57BL/6 mice were allocated into 4 experimental groups. Control group received the standard diet. In group 2, mice received a diet containing 0.2 % (w/w) CPZ mixed with ground chow for a duration of 4 weeks (Zhu et al., 2021). In group 3, mice received Quercetin (150 mg/kg) orally every day for 4 weeks. In group 4, mice received a diet with 0.2 % CPZ and Quercetin (150 mg/kg) orally for 4 weeks. After incidence of the sign of the MS reflexive motor behavior and serum antioxidant levels were assessed. Based on the findings, administration of the CPZ significantly decreased ambulation score, number of crosses using OFT (open field test), stay on rotarod, hindlimb foot angle, front- and hindlimb suspension and grip strength (P &lt; 0.05). Quercetin significantly increased ambulation score, number of cross using, stay on rotarod, hindlimb foot angle, front- and hindlimb suspension and grip strength (P &lt; 0.05). Administration of the CPZ significantly increased serum malondialdehyde (MDA), tumor necrosis factor (TNF-α) and interleukin-1 beta (IL-1β) While decreased superoxide dismutase (SOD), glutathione peroxidase (GPX) and total antioxidant status (TAS) levels (P &lt; 0.05). Quercetin significantly decreased serum MDA, TNF-α and IL-1β while increased SOD, GPX and TAS levels (P &lt; 0.05). These results suggested, Quercetin has protective effect against CPZ-induced MS in mice.</div></div>","PeriodicalId":72753,"journal":{"name":"Current research in physiology","volume":"8 ","pages":"Article 100146"},"PeriodicalIF":2.1000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Protective effect of the Quercetin on experimental cuprizone-induced multiple sclerosis in male C57BL/6 mice\",\"authors\":\"Samin Ghasemi ,&nbsp;Shahin Hassanpour ,&nbsp;Razieh Hosseini\",\"doi\":\"10.1016/j.crphys.2025.100146\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>This study aimed to determine protective effect of the Quercetin on experimental cuprizone-induced (CPZ) multiple sclerosis (MS) in Male C57BL/6 mice. Forty male C57BL/6 mice were allocated into 4 experimental groups. Control group received the standard diet. In group 2, mice received a diet containing 0.2 % (w/w) CPZ mixed with ground chow for a duration of 4 weeks (Zhu et al., 2021). In group 3, mice received Quercetin (150 mg/kg) orally every day for 4 weeks. In group 4, mice received a diet with 0.2 % CPZ and Quercetin (150 mg/kg) orally for 4 weeks. After incidence of the sign of the MS reflexive motor behavior and serum antioxidant levels were assessed. Based on the findings, administration of the CPZ significantly decreased ambulation score, number of crosses using OFT (open field test), stay on rotarod, hindlimb foot angle, front- and hindlimb suspension and grip strength (P &lt; 0.05). Quercetin significantly increased ambulation score, number of cross using, stay on rotarod, hindlimb foot angle, front- and hindlimb suspension and grip strength (P &lt; 0.05). Administration of the CPZ significantly increased serum malondialdehyde (MDA), tumor necrosis factor (TNF-α) and interleukin-1 beta (IL-1β) While decreased superoxide dismutase (SOD), glutathione peroxidase (GPX) and total antioxidant status (TAS) levels (P &lt; 0.05). Quercetin significantly decreased serum MDA, TNF-α and IL-1β while increased SOD, GPX and TAS levels (P &lt; 0.05). These results suggested, Quercetin has protective effect against CPZ-induced MS in mice.</div></div>\",\"PeriodicalId\":72753,\"journal\":{\"name\":\"Current research in physiology\",\"volume\":\"8 \",\"pages\":\"Article 100146\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current research in physiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2665944125000082\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PHYSIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current research in physiology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2665944125000082","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PHYSIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

本研究旨在探讨槲皮素对实验性铜酮诱导的雄性C57BL/6小鼠多发性硬化(MS)的保护作用。将40只雄性C57BL/6小鼠分为4个实验组。对照组给予标准饮食。在第二组中,小鼠接受含有0.2% (w/w) CPZ的日粮与地面饲料混合,持续4周(Zhu et al., 2021)。3组小鼠每日口服槲皮素(150 mg/kg),连续4周。第4组小鼠连续4周口服含0.2% CPZ和槲皮素(150 mg/kg)的日粮。观察MS症状的发生情况及血清抗氧化水平。结果表明,CPZ显著降低了动物的行走评分、野外试验交叉次数、旋转杆停留时间、后肢足角、前肢和后肢悬架以及握力(P <;0.05)。槲皮素显著提高了行走评分、交叉使用次数、停留在旋转杆上、后肢足角、前肢和后肢悬吊和握力(P <;0.05)。CPZ显著提高血清丙二醛(MDA)、肿瘤坏死因子(TNF-α)和白细胞介素-1β (IL-1β),降低超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPX)和总抗氧化状态(TAS)水平(P <;0.05)。槲皮素显著降低血清MDA、TNF-α和IL-1β水平,升高SOD、GPX和TAS水平(P <;0.05)。提示槲皮素对cpz诱导的小鼠多发性硬化症具有保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protective effect of the Quercetin on experimental cuprizone-induced multiple sclerosis in male C57BL/6 mice
This study aimed to determine protective effect of the Quercetin on experimental cuprizone-induced (CPZ) multiple sclerosis (MS) in Male C57BL/6 mice. Forty male C57BL/6 mice were allocated into 4 experimental groups. Control group received the standard diet. In group 2, mice received a diet containing 0.2 % (w/w) CPZ mixed with ground chow for a duration of 4 weeks (Zhu et al., 2021). In group 3, mice received Quercetin (150 mg/kg) orally every day for 4 weeks. In group 4, mice received a diet with 0.2 % CPZ and Quercetin (150 mg/kg) orally for 4 weeks. After incidence of the sign of the MS reflexive motor behavior and serum antioxidant levels were assessed. Based on the findings, administration of the CPZ significantly decreased ambulation score, number of crosses using OFT (open field test), stay on rotarod, hindlimb foot angle, front- and hindlimb suspension and grip strength (P < 0.05). Quercetin significantly increased ambulation score, number of cross using, stay on rotarod, hindlimb foot angle, front- and hindlimb suspension and grip strength (P < 0.05). Administration of the CPZ significantly increased serum malondialdehyde (MDA), tumor necrosis factor (TNF-α) and interleukin-1 beta (IL-1β) While decreased superoxide dismutase (SOD), glutathione peroxidase (GPX) and total antioxidant status (TAS) levels (P < 0.05). Quercetin significantly decreased serum MDA, TNF-α and IL-1β while increased SOD, GPX and TAS levels (P < 0.05). These results suggested, Quercetin has protective effect against CPZ-induced MS in mice.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
3.20
自引率
0.00%
发文量
0
审稿时长
62 days
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信