日本健康成人粪便中粪杆菌的种水平定量研究。

IF 2
Masahiro Hirasaki, Ren Kadowaki, Adeline Ang, Gaku Harata, Kenji Miyazawa, Shintaro Maeno, Miguel Gueimonde, Akihito Endo
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引用次数: 0

摘要

prausnitzii粪杆菌被认为是健康成人肠道微生物群中的主要微生物之一。此外,由于其有益代谢产物和在各种疾病患者中的数量减少,该微生物已被认为是人类健康的关键肠道微生物之一。然而,随着近年来Faecalibacterium属和F. prausnitzii属分类的修订,该物种的种群分布和健康益处变得不清楚。本研究对88例日本健康成人粪便中Faecalibacterium 9个种水平分类类群(以下简称种)的种群进行了种水平的定量分析。qPCR结合基于rpoa的种特异性引物分析结果显示,台湾粪杆菌在粪杆菌属中检出率(流行率)和拷贝数最高,其次为长粪杆菌、duncaniae粪杆菌和prausnitzii粪杆菌,其余5种均偶见。duncaniae种群在不同年龄组间差异显著,40岁和50岁个体高于20岁个体(P分别为0.047和0.002)。本研究表明,在本研究的健康日本成人中,prausnitzii不是主要的粪杆菌种类。未来的研究将阐明Faecalibacterium spp的优势健康益处。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Species-level quantification of <i>Faecalibacterium</i> spp. in faeces of healthy Japanese adults.

Species-level quantification of <i>Faecalibacterium</i> spp. in faeces of healthy Japanese adults.

Species-level quantification of <i>Faecalibacterium</i> spp. in faeces of healthy Japanese adults.

Species-level quantification of Faecalibacterium spp. in faeces of healthy Japanese adults.

Faecalibacterium prausnitzii has been considered one of the predominant microbes in the gut microbiota of healthy human adults. Moreover, due to its beneficial metabolites and its reduced population in patients with various disorders, this organism has been regarded as one of the key gut microbes in human health. However, following recent revisions in the taxonomy of the genus Faecalibacterium and F. prausnitzii, the reported population distribution and health benefits of this species have become unclear. In the present study, the population of nine species-level taxonomic groups (hereafter referred to as species) within Faecalibacterium was quantified at the species level in the faeces of healthy Japanese adults (n=88). qPCR, combined with rpoA-based species-specific primers, showed that Faecalibacterium taiwanense had the highest detection rate (prevalence) and copy number among Faecalibacterium spp., followed by Faecalibacterium longum, Faecalibacterium duncaniae and F. prausnitzii, while the remaining five species were detected only occasionally. The population of F. duncaniae varied significantly between age groups, being higher in individuals in their 40s and 50s compared to those in their 20s (P=0.047 and 0.002, respectively). The present study indicates that F. prausnitzii is not the predominant Faecalibacterium species in the healthy Japanese adults included in the present study. Future studies will shed light on the health benefits of the dominant Faecalibacterium spp.

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