Shan-Shan Li, Shi-Bin Bai, Xiao-Fei Sun, Cheng-Hua Yu, Yi-Ning Tang, Zi-Qiu Jia, Xiao-Ping Li, Song-Yang Shang, David M Irwin, Jun Li, Zhe Wang
{"title":"Zic3抑制四足动物前指发育。","authors":"Shan-Shan Li, Shi-Bin Bai, Xiao-Fei Sun, Cheng-Hua Yu, Yi-Ning Tang, Zi-Qiu Jia, Xiao-Ping Li, Song-Yang Shang, David M Irwin, Jun Li, Zhe Wang","doi":"10.24272/j.issn.2095-8137.2024.360","DOIUrl":null,"url":null,"abstract":"<p><p>Pentadactyl limbs represent a conserved morphological feature among tetrapods, with anterior digits considered more important than posterior digits for refined movement. While posterior digit formation is governed by graded expression of the <i>Shh</i> and 5' <i>Hox</i> genes, the regulatory mechanisms underlying anterior digit development, especially digit I (DI), remain poorly defined. This study identified an anterior expression pattern of <i>Zic3</i> in the limb buds of representative tetrapods, including humans, which exerted an inhibitory effect on skeletal development. <i>Zic3</i> was highly expressed in the anterior region of limb buds at early developmental stages, with species-specific divergence emerging during later development. Overexpression of <i>Zic3</i> significantly delayed chondrogenesis and ossification, leading to bone shortening but not loss. Furthermore, RNA sequencing demonstrated that <i>Zic3</i> down-regulated key genes associated with skeletal development, including <i>Cytl1</i>, <i>Sox9, Ihh</i>, <i>Ptch1</i>, <i>Runx2</i>, and <i>Wnt16</i>. These findings demonstrate that <i>Zic3</i> acts as a conserved inhibitor of anterior skeletal maturation and contributes to the molecular asymmetry of tetrapod limb development.</p>","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"46 3","pages":"684-694"},"PeriodicalIF":4.0000,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Zic3</i> represses anterior digit development in tetrapods.\",\"authors\":\"Shan-Shan Li, Shi-Bin Bai, Xiao-Fei Sun, Cheng-Hua Yu, Yi-Ning Tang, Zi-Qiu Jia, Xiao-Ping Li, Song-Yang Shang, David M Irwin, Jun Li, Zhe Wang\",\"doi\":\"10.24272/j.issn.2095-8137.2024.360\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Pentadactyl limbs represent a conserved morphological feature among tetrapods, with anterior digits considered more important than posterior digits for refined movement. While posterior digit formation is governed by graded expression of the <i>Shh</i> and 5' <i>Hox</i> genes, the regulatory mechanisms underlying anterior digit development, especially digit I (DI), remain poorly defined. This study identified an anterior expression pattern of <i>Zic3</i> in the limb buds of representative tetrapods, including humans, which exerted an inhibitory effect on skeletal development. <i>Zic3</i> was highly expressed in the anterior region of limb buds at early developmental stages, with species-specific divergence emerging during later development. Overexpression of <i>Zic3</i> significantly delayed chondrogenesis and ossification, leading to bone shortening but not loss. Furthermore, RNA sequencing demonstrated that <i>Zic3</i> down-regulated key genes associated with skeletal development, including <i>Cytl1</i>, <i>Sox9, Ihh</i>, <i>Ptch1</i>, <i>Runx2</i>, and <i>Wnt16</i>. These findings demonstrate that <i>Zic3</i> acts as a conserved inhibitor of anterior skeletal maturation and contributes to the molecular asymmetry of tetrapod limb development.</p>\",\"PeriodicalId\":48636,\"journal\":{\"name\":\"Zoological Research\",\"volume\":\"46 3\",\"pages\":\"684-694\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2025-05-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zoological Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.24272/j.issn.2095-8137.2024.360\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zoological Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.24272/j.issn.2095-8137.2024.360","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ZOOLOGY","Score":null,"Total":0}
Zic3 represses anterior digit development in tetrapods.
Pentadactyl limbs represent a conserved morphological feature among tetrapods, with anterior digits considered more important than posterior digits for refined movement. While posterior digit formation is governed by graded expression of the Shh and 5' Hox genes, the regulatory mechanisms underlying anterior digit development, especially digit I (DI), remain poorly defined. This study identified an anterior expression pattern of Zic3 in the limb buds of representative tetrapods, including humans, which exerted an inhibitory effect on skeletal development. Zic3 was highly expressed in the anterior region of limb buds at early developmental stages, with species-specific divergence emerging during later development. Overexpression of Zic3 significantly delayed chondrogenesis and ossification, leading to bone shortening but not loss. Furthermore, RNA sequencing demonstrated that Zic3 down-regulated key genes associated with skeletal development, including Cytl1, Sox9, Ihh, Ptch1, Runx2, and Wnt16. These findings demonstrate that Zic3 acts as a conserved inhibitor of anterior skeletal maturation and contributes to the molecular asymmetry of tetrapod limb development.
期刊介绍:
Established in 1980, Zoological Research (ZR) is a bimonthly publication produced by Kunming Institute of Zoology, the Chinese Academy of Sciences, and the China Zoological Society. It publishes peer-reviewed original research article/review/report/note/letter to the editor/editorial in English on Primates and Animal Models, Conservation and Utilization of Animal Resources, and Animal Diversity and Evolution.