Zic3抑制四足动物前指发育。

IF 4 1区 生物学 Q1 ZOOLOGY
Shan-Shan Li, Shi-Bin Bai, Xiao-Fei Sun, Cheng-Hua Yu, Yi-Ning Tang, Zi-Qiu Jia, Xiao-Ping Li, Song-Yang Shang, David M Irwin, Jun Li, Zhe Wang
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引用次数: 0

摘要

五趾肢在四足动物中代表了一种保守的形态特征,对于精细的运动,前趾比后趾更重要。虽然后趾的形成是由Shh和5' Hox基因的分级表达控制的,但前指发育的调控机制,特别是I趾(DI)的调控机制仍然不明确。本研究发现Zic3在包括人类在内的代表性四足动物的肢芽中具有前向表达模式,对骨骼发育具有抑制作用。Zic3在发育早期在肢芽前区高表达,在发育后期出现物种特异性分化。Zic3的过表达显著延缓了软骨形成和骨化,导致骨缩短但不丢失。此外,RNA测序表明,Zic3下调了与骨骼发育相关的关键基因,包括Cytl1、Sox9、Ihh、Ptch1、Runx2和Wnt16。这些发现表明,Zic3作为一种保守的前骨骼成熟抑制剂,有助于四足动物肢体发育的分子不对称。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Zic3 represses anterior digit development in tetrapods.

Pentadactyl limbs represent a conserved morphological feature among tetrapods, with anterior digits considered more important than posterior digits for refined movement. While posterior digit formation is governed by graded expression of the Shh and 5' Hox genes, the regulatory mechanisms underlying anterior digit development, especially digit I (DI), remain poorly defined. This study identified an anterior expression pattern of Zic3 in the limb buds of representative tetrapods, including humans, which exerted an inhibitory effect on skeletal development. Zic3 was highly expressed in the anterior region of limb buds at early developmental stages, with species-specific divergence emerging during later development. Overexpression of Zic3 significantly delayed chondrogenesis and ossification, leading to bone shortening but not loss. Furthermore, RNA sequencing demonstrated that Zic3 down-regulated key genes associated with skeletal development, including Cytl1, Sox9, Ihh, Ptch1, Runx2, and Wnt16. These findings demonstrate that Zic3 acts as a conserved inhibitor of anterior skeletal maturation and contributes to the molecular asymmetry of tetrapod limb development.

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来源期刊
Zoological Research
Zoological Research Medicine-General Medicine
CiteScore
7.60
自引率
10.20%
发文量
1937
审稿时长
8 weeks
期刊介绍: Established in 1980, Zoological Research (ZR) is a bimonthly publication produced by Kunming Institute of Zoology, the Chinese Academy of Sciences, and the China Zoological Society. It publishes peer-reviewed original research article/review/report/note/letter to the editor/editorial in English on Primates and Animal Models, Conservation and Utilization of Animal Resources, and Animal Diversity and Evolution.
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