共生肠道生物膜在衰老前期的致病性。

IF 7.8 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Guillaume Le Cosquer, Melissa Pannier, Elodie Meunier, Julie Thevenin, Elise Pyhourquet, Sophie Guyonnet, Bruno Vellas, Yohan Santin, Bruno Guiard, Angelo Parini, Louis Buscail, Barbara Bournet, Damien Guillemet, Celine Deraison, Nathalie Vergnolle, Jean-Paul Motta
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引用次数: 0

摘要

不健康衰老的病理生理机制,特别是从健壮到虚弱的转变,仍然知之甚少。尽管对微生物组进行了广泛的分类学研究,但早期脆弱肠道细菌在其自然群落-宿主粘膜组织环境中的行为仍未被探索。使用来自INSPIRE-T衰老人类队列的粪便样本,我们使用多微生物生物膜模型表征了脆弱阶段的肠道微生物群表型。结果显示,与健壮的生物膜相比,前膜衍生的生物膜表现出明显的分类和物理改变,增强了扩散,增加了上皮毒力。多参数分析将生物膜特征与临床特征联系起来,表明它们具有作为衰老状态指标的潜力。在体外,富含多酚的葡萄渣提取物部分逆转了脆弱前生物膜的改变,减少了促炎的脆弱前生物膜反应。来自高龄前小鼠的微生物群诱导抗生素治疗接受者的结肠损伤,建立了虚弱前微生物群-炎症的因果关系。总体而言,研究结果确定了新的体弱前微生物组特征,建立了因果炎症联系,并支持针对体弱前人群的微生物群的老年保护干预措施。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pathogenicity of commensal gut biofilm in prefrail aging.

Pathophysiological mechanisms of unhealthy aging, particularly the transition from robustness to frailty, remain poorly understood. Despite extensive microbiome research on taxonomy, the behavior of early prefrail gut bacteria in their natural community-host mucosal tissue context remains unexplored. Using fecal samples from the INSPIRE-T aging human cohort, we characterized gut microbiota phenotype during prefrailty stages using a polymicrobial biofilm model. Results revealed that prefrail-derived biofilms exhibited distinct taxonomic and physical alterations, enhanced dispersal, and increased epithelial virulence compared to robust counterparts. Multiparametric analyses linked biofilm characteristics to clinical traits, suggesting their potential as aging status indicators. Polyphenol-rich grape pomace extract partially reversed prefrail biofilm alterations and reduced proinflammatory prefrail biofilm responses in vitro. Microbiota from prefrail-aged mice induced colon damage in antibiotic-treated recipients, establishing a prefrail microbiome-inflammation causality. Overall, the findings identified novel prefrail microbiome characteristics, established causal inflammatory links, and supported microbiota-targeted geroprotective interventions for the prefrail populations.

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来源期刊
npj Biofilms and Microbiomes
npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology
CiteScore
12.10
自引率
3.30%
发文量
91
审稿时长
9 weeks
期刊介绍: npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.
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