LINC01158在乳腺癌中的临床意义及通过调节miR-711抑制乳腺癌细胞的增殖转移。

IF 2.5 3区 生物学
Xiaohe Lan, Zibai Guo, Linmei Lin, Wanqi Lin, Yi Zheng, Yabing Liu
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引用次数: 0

摘要

目的:乳腺癌(BC)由于转移和复发,预后较差。LINC01158在乳腺癌中异常表达。因此,我们研究了LINC01158在BC中的调节机制和预后价值。方法:121例BC患者入组。RT-qPCR分析LINC01158和miR-711水平。采用多因素Cox回归分析BC不良预后的独立预测因素。采用Kaplan-Meier曲线分析BC患者的5年生存率。DLR检测证实了LINC01158与miR-711靶标结合之间的关系。CCK-8检测细胞的增殖能力。用Transwell分析细胞迁移和侵袭能力。结果:在BC组织和细胞系中,LINC01158表达降低,miR-711水平升高。LINC01158的低表达导致BC患者的总生存期缩短。LINC01158结合miR-711靶点,与miR-711水平呈负相关。过表达LINC01158可降低miR-711水平,减少BC细胞的增殖、迁移和侵袭。此外,Cox回归结果显示,LINC01158是BC的独立预后因素。结论:LINC01158可能是BC的预后指标。升高LINC01158的表达水平可以降低miR-711的表达,从而抑制细胞的增殖、迁移和侵袭行为,具有延缓BC进展的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Clinical significance of LINC01158 in breast cancer and inhibition of proliferation and metastasis of breast cancer cells by regulating miR-711.

Objective: Breast cancer (BC) has a poor prognosis due to metastasis and recurrence. LINC01158 is aberrantly expressed in breast cancer. Therefore, we investigated the regulatory mechanism and prognostic value of LINC01158 in BC.

Methods: 121 patients with BC were enrolled. LINC01158 and miR-711 levels were analyzed by RT-qPCR. Independent predictors of poor BC prognosis were analyzed by multifactorial Cox regression. Kaplan-Meier curves were used to analyze the 5-year survival rate of BC patients. DLR assay verified the relationship between LINC01158 and miR-711 target binding. CCK-8 was used to detect the proliferative capacity of cells. Transwell was used to analyze cell migration and invasion ability.

Results: In BC tissues and cell lines, LINC01158 expression was reduced and miR-711 levels were elevated. Low expression of LINC01158 resulted in a shortened overall survival of BC patients. LINC01158 binds to the miR-711 target and negatively correlates with the level of miR-711. Overexpression of LINC01158 decreased miR-711 levels and reduced BC cell proliferation, migration and invasion. In addition, Cox regression results showed that LINC01158 was an independent prognostic factor for BC.

Conclusion: LINC01158 may be a prognostic marker for BC. Increasing the expression level of LINC01158 could reduce the expression of miR-711, which could inhibit cell proliferation, migration and invasive behaviors, and has the potential to delay the progression of BC.

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来源期刊
Hereditas
Hereditas Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
3.80
自引率
3.70%
发文量
0
期刊介绍: For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.
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