Mirian Saavedra Lopes Ururahy, Erick Silva Barbosa, Rafaela Manente, Ana Paula Ramos, Antônio Eduardo Miller Crotti, Silmara Aparecida Milori Corona
{"title":"磷酸化壳聚糖和碳二亚胺对侵蚀牙本质表面润湿性、表面自由能和表面形貌的影响。","authors":"Mirian Saavedra Lopes Ururahy, Erick Silva Barbosa, Rafaela Manente, Ana Paula Ramos, Antônio Eduardo Miller Crotti, Silmara Aparecida Milori Corona","doi":"10.17219/dmp/170245","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Biomodifiers can reinforce the collagen matrix, improving the biomechanical and biochemical properties of dentin.</p><p><strong>Objectives: </strong>The present study aimed to evaluate how 2.5% phosphorylated chitosan (P-Chi) and 0.5 mol/L carbodiimide (EDC) affect the surface wettability, surface free energy and surface morphology of the eroded dentin.</p><p><strong>Material and methods: </strong>Bovine tooth specimens (N = 144) were randomly divided into 6 groups according to the dentin substrate (sound or eroded) and surface treatment (2.5% P-Chi, 0.5 mol/L EDC or no biomodification (control)). Half of the specimens (n = 72) were submitted to erosive challenge by immersion in 0.3% citric acid (pH 3.2) for 2 h. For the surface wettability analysis (n = 12), the contact angles between the dentin surface and the 3M™ Scotchbond™ Universal adhesive were measured with a goniometer. For the surface free energy analysis (n = 3), the contact angles between the dentin surface and 3 organic solvents of distinct polarities (water, formamide and diiodomethane) were recorded. Surface morphology (n = 3) was analyzed with the use of scanning electron microscopy (SEM). The data was statistically analyzed using the two-way analysis of variance (ANOVA) (α = 0.05).</p><p><strong>Results: </strong>Neither 2.5% P-Chi nor 0.5 mol/L EDC influenced the dentin surface wettability (p > 0.05). Surface free energy decreased in the eroded substrate after biomodification with EDC (p < 0.05). Biomodification with P-Chi demineralized the dentin surface and increased the dentin tubule embouchure.</p><p><strong>Conclusions: </strong>It can be concluded that 2.5% P-Chi and 0.5 mol/L EDC did not impact the surface wettability of the eroded dentin. However, EDC promoted lower surface free energy, while P-Chi altered surface morphology, causing demineralization and the opening of dentin tubules.</p>","PeriodicalId":11191,"journal":{"name":"Dental and Medical Problems","volume":"62 2","pages":"275-283"},"PeriodicalIF":3.9000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of phosphorylated chitosan and carbodiimide on the surface wettability, surface free energy and surface morphology of the eroded dentin.\",\"authors\":\"Mirian Saavedra Lopes Ururahy, Erick Silva Barbosa, Rafaela Manente, Ana Paula Ramos, Antônio Eduardo Miller Crotti, Silmara Aparecida Milori Corona\",\"doi\":\"10.17219/dmp/170245\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Biomodifiers can reinforce the collagen matrix, improving the biomechanical and biochemical properties of dentin.</p><p><strong>Objectives: </strong>The present study aimed to evaluate how 2.5% phosphorylated chitosan (P-Chi) and 0.5 mol/L carbodiimide (EDC) affect the surface wettability, surface free energy and surface morphology of the eroded dentin.</p><p><strong>Material and methods: </strong>Bovine tooth specimens (N = 144) were randomly divided into 6 groups according to the dentin substrate (sound or eroded) and surface treatment (2.5% P-Chi, 0.5 mol/L EDC or no biomodification (control)). Half of the specimens (n = 72) were submitted to erosive challenge by immersion in 0.3% citric acid (pH 3.2) for 2 h. For the surface wettability analysis (n = 12), the contact angles between the dentin surface and the 3M™ Scotchbond™ Universal adhesive were measured with a goniometer. For the surface free energy analysis (n = 3), the contact angles between the dentin surface and 3 organic solvents of distinct polarities (water, formamide and diiodomethane) were recorded. Surface morphology (n = 3) was analyzed with the use of scanning electron microscopy (SEM). The data was statistically analyzed using the two-way analysis of variance (ANOVA) (α = 0.05).</p><p><strong>Results: </strong>Neither 2.5% P-Chi nor 0.5 mol/L EDC influenced the dentin surface wettability (p > 0.05). Surface free energy decreased in the eroded substrate after biomodification with EDC (p < 0.05). Biomodification with P-Chi demineralized the dentin surface and increased the dentin tubule embouchure.</p><p><strong>Conclusions: </strong>It can be concluded that 2.5% P-Chi and 0.5 mol/L EDC did not impact the surface wettability of the eroded dentin. However, EDC promoted lower surface free energy, while P-Chi altered surface morphology, causing demineralization and the opening of dentin tubules.</p>\",\"PeriodicalId\":11191,\"journal\":{\"name\":\"Dental and Medical Problems\",\"volume\":\"62 2\",\"pages\":\"275-283\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2025-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Dental and Medical Problems\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17219/dmp/170245\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Dental and Medical Problems","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17219/dmp/170245","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Effect of phosphorylated chitosan and carbodiimide on the surface wettability, surface free energy and surface morphology of the eroded dentin.
Background: Biomodifiers can reinforce the collagen matrix, improving the biomechanical and biochemical properties of dentin.
Objectives: The present study aimed to evaluate how 2.5% phosphorylated chitosan (P-Chi) and 0.5 mol/L carbodiimide (EDC) affect the surface wettability, surface free energy and surface morphology of the eroded dentin.
Material and methods: Bovine tooth specimens (N = 144) were randomly divided into 6 groups according to the dentin substrate (sound or eroded) and surface treatment (2.5% P-Chi, 0.5 mol/L EDC or no biomodification (control)). Half of the specimens (n = 72) were submitted to erosive challenge by immersion in 0.3% citric acid (pH 3.2) for 2 h. For the surface wettability analysis (n = 12), the contact angles between the dentin surface and the 3M™ Scotchbond™ Universal adhesive were measured with a goniometer. For the surface free energy analysis (n = 3), the contact angles between the dentin surface and 3 organic solvents of distinct polarities (water, formamide and diiodomethane) were recorded. Surface morphology (n = 3) was analyzed with the use of scanning electron microscopy (SEM). The data was statistically analyzed using the two-way analysis of variance (ANOVA) (α = 0.05).
Results: Neither 2.5% P-Chi nor 0.5 mol/L EDC influenced the dentin surface wettability (p > 0.05). Surface free energy decreased in the eroded substrate after biomodification with EDC (p < 0.05). Biomodification with P-Chi demineralized the dentin surface and increased the dentin tubule embouchure.
Conclusions: It can be concluded that 2.5% P-Chi and 0.5 mol/L EDC did not impact the surface wettability of the eroded dentin. However, EDC promoted lower surface free energy, while P-Chi altered surface morphology, causing demineralization and the opening of dentin tubules.