活化杏鲍菇多糖:伽马辐射增强抗糖尿病和抗氧化潜能。

IF 5.1 3区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Eman H F Abd El-Zaher, Ehab M Tousson, Azza A Mostafa, Enas M El-Gaar, Galal Yahya, Yehia A-G Mahmoud
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引用次数: 0

摘要

从杏鲍菇中提取的多糖具有多种生物活性,包括抗氧化、降糖、抗炎和免疫调节作用。在本研究中,我们从双生子实体中提取多糖,分别在50和100 kGy的剂量下照射,剂量率为5 kGy/h。与50 kGy辐照和未辐照样品相比,100 kGy辐照后的多糖表面形貌显示出大量的孔隙和较小的片状结构。所有样品的1H和13C NMR谱表明,辐照和未辐照多糖均表现为α-和β-构型,并清晰地观察到C1-C5对应的信号。HPLC分析结果表明,多糖的主要成分为葡萄糖(75.23%)、半乳糖(4.96%)、葡萄糖醛酸(1.38%)、核糖(0.94%)、鼠李糖(2.35%)和甘露糖(3.87%)。各多糖均表现出抗氧化活性,且抗氧化活性随浓度的增加而增强。未辐照多糖和辐照多糖均表现出抗糖尿病作用,显著降低血糖水平,恢复胰岛素水平,且辐照多糖具有优势。此外,与糖尿病对照组相比,它们显著提高了治疗大鼠的体重,略微降低了MDA水平,并显著提高了过氧化氢酶活性。多糖对糖尿病大鼠胰腺和肝脏的组织病理学检查进一步证实了多糖的抗糖尿病作用。这表明,辐照通过降低多糖的分子量,提高其生物利用度和调节葡萄糖代谢的功效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Revitalizing Pleurotus eryngii polysaccharides: gamma irradiation boosts antidiabetic and antioxidant potential.

Polysaccharides derived from Pleurotus eryngii possess various bioactive properties, including antioxidant, antidiabetic, anti-inflammatory, and immunomodulatory effects. In this study, polysaccharides were extracted from P. eryngii fruiting bodies and exposed to gamma irradiation at doses of 50 and 100 kGy, with a dose rate of 5 kGy/h. The surface morphology of the polysaccharide irradiated at 100 kGy exhibited numerous pores and a smaller flake structure compared to those irradiated at 50 kGy and the non-irradiated sample. 1H and 13C NMR spectra of all samples indicated that both irradiated and non-irradiated polysaccharides exhibited α- and β-configurations, with signals corresponding to C1-C5 clearly observed. HPLC analysis of the polysaccharides revealed that glucose (75.23%), galactose (4.96%), glucuronic acid (1.38%), ribose (0.94%), rhamnose (2.35%), and mannose (3.87%) are the main components. All polysaccharides demonstrated antioxidant activity, which increased with concentration. Both non-irradiated and irradiated polysaccharides exhibited antidiabetic effects, significantly reducing blood glucose levels, and restoring insulin level with superiority of irradiated polysaccharides. Additionally, they significantly elevated body weight, slightly reduced MDA levels, and markedly enhanced catalase activity in treated rats compared to diabetic controls. The antidiabetic effects of the polysaccharides were further confirmed by histopathological examination of the pancreas and liver sections from polysaccharide-treated diabetic rats. This suggests that irradiation, by reducing the molecular weight of polysaccharides, enhances their bioavailability and efficacy in modulating glucose metabolism.

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来源期刊
Bioresources and Bioprocessing
Bioresources and Bioprocessing BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
8.70%
发文量
118
审稿时长
13 weeks
期刊介绍: Bioresources and Bioprocessing (BIOB) is a peer-reviewed open access journal published under the brand SpringerOpen. BIOB aims at providing an international academic platform for exchanging views on and promoting research to support bioresource development, processing and utilization in a sustainable manner. As an application-oriented research journal, BIOB covers not only the application and management of bioresource technology but also the design and development of bioprocesses that will lead to new and sustainable production processes. BIOB publishes original and review articles on most topics relating to bioresource and bioprocess engineering, including: -Biochemical and microbiological engineering -Biocatalysis and biotransformation -Biosynthesis and metabolic engineering -Bioprocess and biosystems engineering -Bioenergy and biorefinery -Cell culture and biomedical engineering -Food, agricultural and marine biotechnology -Bioseparation and biopurification engineering -Bioremediation and environmental biotechnology
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