Robert Barber, Taylor S. Cunning, Victoria Gilpin, Martin McBride, Nigel G. Ternan, William J. Snelling, Pagona Papakonstantinou, James Davis
{"title":"石墨烯-普鲁士蓝-壳聚糖复合传感器在约氏乳杆菌DSM 10533培养物中过氧化氢监测的研制","authors":"Robert Barber, Taylor S. Cunning, Victoria Gilpin, Martin McBride, Nigel G. Ternan, William J. Snelling, Pagona Papakonstantinou, James Davis","doi":"10.1007/s10008-025-06260-x","DOIUrl":null,"url":null,"abstract":"<div><p>The design and application of a composite sensor for monitoring bacterial peroxide originating from <i>Lactobacillus johnsonii</i> DSM 10533 is described. The sensor format is based on the co-deposition of Prussian blue and chitosan onto a laser-induced graphene substrate to yield a highly sensitive system capable of monitoring peroxide with bacterial culture over the range 20 µM to 1 mM with a sensitivity of 122 mA/mM and detection limit of 30 mM. Amperometric detection of H<sub>2</sub>O<sub>2</sub> at − 0.036 V vs Ag/AgCl was achieved allowing selectivity in complex media. The miniature nature of the sensor enabled a detection volume of 200 µL and gave a response in 20 s, and the samples required no dilution or reagent addition. The ability to rapidly prototype LIG-PB-CS integrated electrode assemblies provides a facile route for the production of sensors capable of monitoring lactobacilli interactions at small scales and within batch-level fermentation reactors.</p></div>","PeriodicalId":665,"journal":{"name":"Journal of Solid State Electrochemistry","volume":"29 2024","pages":"2351 - 2359"},"PeriodicalIF":2.6000,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10008-025-06260-x.pdf","citationCount":"0","resultStr":"{\"title\":\"Development of a composite graphene-Prussian blue-chitosan sensor for monitoring peroxide within Lactobacillus johnsonii DSM 10533 cultures\",\"authors\":\"Robert Barber, Taylor S. Cunning, Victoria Gilpin, Martin McBride, Nigel G. Ternan, William J. Snelling, Pagona Papakonstantinou, James Davis\",\"doi\":\"10.1007/s10008-025-06260-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The design and application of a composite sensor for monitoring bacterial peroxide originating from <i>Lactobacillus johnsonii</i> DSM 10533 is described. The sensor format is based on the co-deposition of Prussian blue and chitosan onto a laser-induced graphene substrate to yield a highly sensitive system capable of monitoring peroxide with bacterial culture over the range 20 µM to 1 mM with a sensitivity of 122 mA/mM and detection limit of 30 mM. Amperometric detection of H<sub>2</sub>O<sub>2</sub> at − 0.036 V vs Ag/AgCl was achieved allowing selectivity in complex media. The miniature nature of the sensor enabled a detection volume of 200 µL and gave a response in 20 s, and the samples required no dilution or reagent addition. The ability to rapidly prototype LIG-PB-CS integrated electrode assemblies provides a facile route for the production of sensors capable of monitoring lactobacilli interactions at small scales and within batch-level fermentation reactors.</p></div>\",\"PeriodicalId\":665,\"journal\":{\"name\":\"Journal of Solid State Electrochemistry\",\"volume\":\"29 2024\",\"pages\":\"2351 - 2359\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-03-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://link.springer.com/content/pdf/10.1007/s10008-025-06260-x.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Solid State Electrochemistry\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10008-025-06260-x\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ELECTROCHEMISTRY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Solid State Electrochemistry","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10008-025-06260-x","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ELECTROCHEMISTRY","Score":null,"Total":0}
引用次数: 0
摘要
介绍了一种用于监测约氏乳杆菌DSM 10533过氧化细菌的复合传感器的设计和应用。传感器格式是基于在激光诱导的石墨烯衬底上共同沉积的Prussian蓝和壳聚糖，从而产生一个高灵敏度的系统，能够监测细菌培养在20µM至1 mM范围内的过氧化物，灵敏度为122 mA/mM，检测限为30 mM。在- 0.036 V vs Ag/AgCl下实现H2O2的安培检测，允许在复杂介质中进行选择性。传感器的微型特性使其检测体积为200µL，并在20 s内给出响应，并且样品无需稀释或添加试剂。快速原型化ligl - pb - cs集成电极组件的能力为能够在小规模和批量发酵反应器中监测乳酸菌相互作用的传感器的生产提供了便捷的途径。
Development of a composite graphene-Prussian blue-chitosan sensor for monitoring peroxide within Lactobacillus johnsonii DSM 10533 cultures
The design and application of a composite sensor for monitoring bacterial peroxide originating from Lactobacillus johnsonii DSM 10533 is described. The sensor format is based on the co-deposition of Prussian blue and chitosan onto a laser-induced graphene substrate to yield a highly sensitive system capable of monitoring peroxide with bacterial culture over the range 20 µM to 1 mM with a sensitivity of 122 mA/mM and detection limit of 30 mM. Amperometric detection of H2O2 at − 0.036 V vs Ag/AgCl was achieved allowing selectivity in complex media. The miniature nature of the sensor enabled a detection volume of 200 µL and gave a response in 20 s, and the samples required no dilution or reagent addition. The ability to rapidly prototype LIG-PB-CS integrated electrode assemblies provides a facile route for the production of sensors capable of monitoring lactobacilli interactions at small scales and within batch-level fermentation reactors.
期刊介绍：
The Journal of Solid State Electrochemistry is devoted to all aspects of solid-state chemistry and solid-state physics in electrochemistry.
The Journal of Solid State Electrochemistry publishes papers on all aspects of electrochemistry of solid compounds, including experimental and theoretical, basic and applied work. It equally publishes papers on the thermodynamics and kinetics of electrochemical reactions if at least one actively participating phase is solid. Also of interest are articles on the transport of ions and electrons in solids whenever these processes are relevant to electrochemical reactions and on the use of solid-state electrochemical reactions in the analysis of solids and their surfaces.
The journal covers solid-state electrochemistry and focusses on the following fields: mechanisms of solid-state electrochemical reactions, semiconductor electrochemistry, electrochemical batteries, accumulators and fuel cells, electrochemical mineral leaching, galvanic metal plating, electrochemical potential memory devices, solid-state electrochemical sensors, ion and electron transport in solid materials and polymers, electrocatalysis, photoelectrochemistry, corrosion of solid materials, solid-state electroanalysis, electrochemical machining of materials, electrochromism and electrochromic devices, new electrochemical solid-state synthesis.
The Journal of Solid State Electrochemistry makes the professional in research and industry aware of this swift progress and its importance for future developments and success in the above-mentioned fields.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
