干旱通过下调NAC转录因子ICS1的表达来抑制植物水杨酸对草食性昆虫的防御

IF 6.8 Q1 PLANT SCIENCES

Plant Stress Pub Date : 2025-05-12 DOI:10.1016/j.stress.2025.100887

Chan Zhao , Wen-Hao Han , Yu-Die Xiong, Shun-Xia Ji, Hui Du, Yu-Jie Chi, Na Chen, He Wu, Shu-Sheng Liu, Xiao-Wei Wang

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引用次数: 0

摘要

许多草食性昆虫在干旱胁迫的寄主植物上表现出增强的性能和种群动态，这是由于植物生理诱导的变化。然而，干旱如何调节植物防御草食性昆虫的潜在机制在很大程度上仍然未知。本研究发现，全球害虫白蝇在干旱胁迫烟草上的成活率和繁殖力显著高于干旱胁迫烟草。干旱胁迫不影响茉莉酸（jasmonic acid， JA）的积累，但通过抑制水杨酸（salicylic acid， SA）的生物合成，显著降低了水杨酸（salicylic acid， SA）的含量。我们进一步证明，干旱诱导的脱落酸（ABA）抑制了异氯酸酯合成酶1 （ICS1）的表达，ICS1是SA合成的关键酶。ABA的积累诱导脱落酸响应性NAC结构域蛋白19 （ANAC019）的表达，ANAC019直接结合NtICS1的启动子并负向调控其表达。最后，我们发现干旱和ABA可以激活脱落酸响应元件结合因子2 （ABF2）转录因子上调NtANAC019的表达。我们的研究阐明了非生物胁迫与植物-食草动物相互作用之间的交叉对话的重要性，并揭示了导致食草动物适应干旱胁迫植物改变的机制。

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Drought suppresses plant salicylic acid defence against herbivorous insects by down-regulating the expression of ICS1 via NAC transcription factor

Many herbivorous insects exhibit enhanced performance and population dynamics on drought-stressed host plants due to induced changes in plant physiology. However, the underlying mechanisms of how drought regulates plant defense against herbivorous insects are still largely unknown. In this study, we found that the survival rate and fecundity of whiteflies, notorious global pests, were significantly higher on drought-stressed tobacco. Drought stress did not affect jasmonic acid (JA) accumulation, whereas it significantly decreased the salicylic acid (SA) content by suppressing its biosynthesis. We further demonstrated that drought-induced abscisic acid (ABA) inhibited the expression of isochorismate synthase 1 (ICS1), a key enzyme in the synthesis of SA. The accumulation of ABA induced the expression of abscisic acid responsive NAC domain containing protein 19 (ANAC019), which directly bound to the promoter of NtICS1 and negatively regulated its expression. Finally, we revealed that drought and ABA could activate abscisic acid responsive elements-binding factor 2 (ABF2) transcription factor to up-regulate the expression of NtANAC019. Our study illustrates the significance of cross-talk between abiotic stress and plant-herbivore interactions and reveals the mechanisms leading to altered herbivore fitness on drought-stressed plants.

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.