协同微藻-细菌相互作用增强曝气膜生物膜光反应器处理盐胁迫下水产养殖废水的氮去除：来自宏基因组分析的见解

IF 11.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Water Research Pub Date : 2025-05-22 DOI:10.1016/j.watres.2025.123878

Zhengang Xia , How Yong Ng , Sungwoo Bae

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引用次数: 0

摘要

研究了曝气膜生物膜光反应器（MABPR）处理低碳氮比、高盐度（0.5% ~ 3.2%）水产养殖出水的效果。MABPR将生物膜反应器与微藻-细菌联合体结合在一起，通过利用反扩散生物膜特性、无气泡曝气和增强的微藻生产力，实现了卓越的总无机氮（TIN）去除。与SBBPR和MABR相比，MABPR促进了微藻的增殖（Chl-a/VSS: 8.08-15.04 mg/g）和生物质生产力（1.83 g/m²d）， TIN去除率从0.82±0.04提高到1.22±0.07 g/m²d，在3.2%盐度下TIN去除率达到84.7±1.9%。升高的盐度刺激细胞外聚合物（EPS）的产生，增强生物膜的稳定性和微生物的恢复力。在最高盐度下，MABPR的脱氮效率比对照高出22%-65%。典型硝化-反硝化仍然是主要的脱氮途径，在盐胁迫下，短程硝化-反硝化也起作用。宏基因组分析揭示了微藻与细菌之间的双向适应，通过丰富的氮同化（GS/GOGAT途径）弥补细菌的缺陷。微藻通过氨吸收和溶解有机物释放促进污染物去除，支持反硝化作用。在3.2%盐度下，亚硝化单胞菌和硝化杆菌的丰度分别增加了42.6%和35.8%，而反硝化菌反硝化单胞菌和Hoeflea的丰度分别占59.4%和35.9%。MABPR进一步促进了生长辅助因子（维生素、植物激素）的合成，提高了微藻的生产力和抗逆性。这些协同的微藻-细菌相互作用支持污染物去除，表明MABPR是盐胁迫下水产养殖废水处理和资源回收的稳健、可持续的解决方案。

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Synergistic microalgal-bacterial interactions enhance nitrogen removal in membrane-aerated biofilm photoreactors treating aquaculture wastewater under salt stress: Insights from metagenomic analysis

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Synergistic microalgal-bacterial interactions enhance nitrogen removal in membrane-aerated biofilm photoreactors treating aquaculture wastewater under salt stress: Insights from metagenomic analysis

This study investigates the membrane-aerated biofilm photoreactor (MABPR) for treating aquaculture effluents with low C/N ratio and elevated salinity (0.5%–3.2%). The MABPR integrated biofilm reactors with microalgal-bacterial consortia, achieving superior total inorganic nitrogen (TIN) removal by leveraging counter-diffusional biofilm properties, bubbleless aeration, and enhanced microalgal productivity. The system consistently outperformed conventional reactors, achieving 84.7 ± 1.9% TIN removal at 3.2% salinity with TIN removal flux increasing from 0.82 ± 0.04 to 1.22 ± 0.07 g/m² d. The MABPR promoted microalgal proliferation (Chl-a/VSS: 8.08-15.04 mg/g) and higher biomass productivity (1.83 g/m² d) compared to SBBPR and MABR. Elevated salinity stimulated extracellular polymeric substance (EPS) production, reinforcing biofilm stability and microbial resilience. The MABPR demonstrated 22%–65% higher nitrogen removal efficiency than controls at the highest salinity. Canonical nitrification-denitrification remained the primary nitrogen removal pathway, with short-cut nitrification-denitrification contributing under salt stress. Metagenomic analysis revealed bidirectional adaptation between microalgae and bacteria, with enriched nitrogen assimilation (GS/GOGAT pathway) compensating for bacterial deficits. Microalgae facilitated pollutant removal through ammonia uptake and dissolved organic matter release, supporting denitrification. At 3.2% salinity, Nitrosomonas and Nitrobacter abundance increased by 42.6% and 35.8%, while denitrifiers Denitromonas and Hoeflea dominated, comprising 59.4% and 35.9% of the population. The MABPR further promoted the synthesis of growth cofactors (vitamins, phytohormones), enhancing microalgal productivity and stress resilience. These synergistic microalgal-bacterial interactions supported pollutant removal, showcasing the MABPR as a robust, sustainable solution for aquaculture wastewater treatment and resource recovery under salt stress.

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来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.