Maria Luisa Pardiñas, Rocío Rivera-Egea, Jose Maria de Los Santos, Carmen Vidal, Juan Giles, David Ortega-Jaen, Julia Gil, Angel Martin, Thamara Viloria, Maria Jose de Los Santos
{"title":"基于微流体的选择装置对精子DNA断裂和icsi周期的影响。","authors":"Maria Luisa Pardiñas, Rocío Rivera-Egea, Jose Maria de Los Santos, Carmen Vidal, Juan Giles, David Ortega-Jaen, Julia Gil, Angel Martin, Thamara Viloria, Maria Jose de Los Santos","doi":"10.1016/j.xfss.2025.05.002","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Determine the impact of a microfluidic-based sperm selection device on sperm parameters and embryo variables compared to the conventional swim-up method in sibling oocytes.</p><p><strong>Design: </strong>Prospective observational study.</p><p><strong>Subjects: </strong>A total of 345 oocytes were recruited from 27 couples undergoing intracytoplasmic sperm injection (ICSI), including both own (n=195) and donation (n=150) cycles. None of the patients presented severe male factor.</p><p><strong>Intervention/exposure: </strong>Semen sample was divided into two groups and processed using (1) swim-up or (2) microfluidic sperm selection device (MSSD) ZyMōt. Half of the oocytes were inseminated with swim-up-selected spermatozoa, and the rest with MSSD-selected spermatozoa. Embryo development was followed by time-lapse. SDF was measured using sperm chromatin dispersion test, analyzed with ImageJ. A Mann-Whitney U test was performed for statistical analysis.</p><p><strong>Main outcome measures: </strong>Sperm DNA fragmentation (SDF) levels, sperm parameters, fertilization rates, embryo morphokinetics and the rate of usable blastocysts.</p><p><strong>Results: </strong>SDF was significantly lower in MSSD group compared to swim-up group (20.3% vs. 11%; P=0.004), indicating a better selection. Analyzing separately the oocytes from the patient's own cycles, MSSD showed a significantly higher rate of usable blastocysts per fertilized oocyte (P=0.041). However, this difference was not observed using donated oocytes or when both cycles were combined (P>0.05). Embryos from swim-up group showed a significant delay in time of pronuclei appearance (tPNa) and morula formation (tM) compared to MSSD group, being more marked in donated oocytes (P<0.05). No significant differences were observed in fertilization rate and the remaining morphokinetic times (P>0.05).</p><p><strong>Conclusion: </strong>This study provides valuable information on the use of MSSD for non-invasive sperm selection. When MSSD was used, we have observed a reduction in SDF and an enhancement in the number of usable embryos in our own cycles. These findings could be compatible with a reduced capacity to repair sperm damage due to poorer oocyte quality caused by advanced age.</p>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"IMPACT OF A MICROFLUIDIC-BASED SELECTION DEVICE ON SPERM DNA FRAGMENTATION AND ICSI CYCLES.\",\"authors\":\"Maria Luisa Pardiñas, Rocío Rivera-Egea, Jose Maria de Los Santos, Carmen Vidal, Juan Giles, David Ortega-Jaen, Julia Gil, Angel Martin, Thamara Viloria, Maria Jose de Los Santos\",\"doi\":\"10.1016/j.xfss.2025.05.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Determine the impact of a microfluidic-based sperm selection device on sperm parameters and embryo variables compared to the conventional swim-up method in sibling oocytes.</p><p><strong>Design: </strong>Prospective observational study.</p><p><strong>Subjects: </strong>A total of 345 oocytes were recruited from 27 couples undergoing intracytoplasmic sperm injection (ICSI), including both own (n=195) and donation (n=150) cycles. None of the patients presented severe male factor.</p><p><strong>Intervention/exposure: </strong>Semen sample was divided into two groups and processed using (1) swim-up or (2) microfluidic sperm selection device (MSSD) ZyMōt. Half of the oocytes were inseminated with swim-up-selected spermatozoa, and the rest with MSSD-selected spermatozoa. Embryo development was followed by time-lapse. SDF was measured using sperm chromatin dispersion test, analyzed with ImageJ. A Mann-Whitney U test was performed for statistical analysis.</p><p><strong>Main outcome measures: </strong>Sperm DNA fragmentation (SDF) levels, sperm parameters, fertilization rates, embryo morphokinetics and the rate of usable blastocysts.</p><p><strong>Results: </strong>SDF was significantly lower in MSSD group compared to swim-up group (20.3% vs. 11%; P=0.004), indicating a better selection. Analyzing separately the oocytes from the patient's own cycles, MSSD showed a significantly higher rate of usable blastocysts per fertilized oocyte (P=0.041). However, this difference was not observed using donated oocytes or when both cycles were combined (P>0.05). Embryos from swim-up group showed a significant delay in time of pronuclei appearance (tPNa) and morula formation (tM) compared to MSSD group, being more marked in donated oocytes (P<0.05). No significant differences were observed in fertilization rate and the remaining morphokinetic times (P>0.05).</p><p><strong>Conclusion: </strong>This study provides valuable information on the use of MSSD for non-invasive sperm selection. When MSSD was used, we have observed a reduction in SDF and an enhancement in the number of usable embryos in our own cycles. 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IMPACT OF A MICROFLUIDIC-BASED SELECTION DEVICE ON SPERM DNA FRAGMENTATION AND ICSI CYCLES.
Objective: Determine the impact of a microfluidic-based sperm selection device on sperm parameters and embryo variables compared to the conventional swim-up method in sibling oocytes.
Design: Prospective observational study.
Subjects: A total of 345 oocytes were recruited from 27 couples undergoing intracytoplasmic sperm injection (ICSI), including both own (n=195) and donation (n=150) cycles. None of the patients presented severe male factor.
Intervention/exposure: Semen sample was divided into two groups and processed using (1) swim-up or (2) microfluidic sperm selection device (MSSD) ZyMōt. Half of the oocytes were inseminated with swim-up-selected spermatozoa, and the rest with MSSD-selected spermatozoa. Embryo development was followed by time-lapse. SDF was measured using sperm chromatin dispersion test, analyzed with ImageJ. A Mann-Whitney U test was performed for statistical analysis.
Main outcome measures: Sperm DNA fragmentation (SDF) levels, sperm parameters, fertilization rates, embryo morphokinetics and the rate of usable blastocysts.
Results: SDF was significantly lower in MSSD group compared to swim-up group (20.3% vs. 11%; P=0.004), indicating a better selection. Analyzing separately the oocytes from the patient's own cycles, MSSD showed a significantly higher rate of usable blastocysts per fertilized oocyte (P=0.041). However, this difference was not observed using donated oocytes or when both cycles were combined (P>0.05). Embryos from swim-up group showed a significant delay in time of pronuclei appearance (tPNa) and morula formation (tM) compared to MSSD group, being more marked in donated oocytes (P<0.05). No significant differences were observed in fertilization rate and the remaining morphokinetic times (P>0.05).
Conclusion: This study provides valuable information on the use of MSSD for non-invasive sperm selection. When MSSD was used, we have observed a reduction in SDF and an enhancement in the number of usable embryos in our own cycles. These findings could be compatible with a reduced capacity to repair sperm damage due to poorer oocyte quality caused by advanced age.
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