Andrew C Markel, Cameron Hoerig, Allan K N de Alencar, Kenneth F Swan, Alexander D Gleed, Lili Shi, Gabriella Pridjian, Jonathan Mamou, Carolyn L Bayer
{"title":"子痫前期大鼠模型胎盘组织微观结构的定量超声表征。","authors":"Andrew C Markel, Cameron Hoerig, Allan K N de Alencar, Kenneth F Swan, Alexander D Gleed, Lili Shi, Gabriella Pridjian, Jonathan Mamou, Carolyn L Bayer","doi":"10.1016/j.ultrasmedbio.2025.04.017","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Quantitative ultrasound (QUS) methods were applied to detect changes in placental microstructure in the reduced uterine perfusion pressure (RUPP) model of preeclampsia in rats. Preeclampsia is a life-threatening pregnancy disorder associated with abnormal placental development that is inadequately treated and managed.</p><p><strong>Methods: </strong>Nine timed-pregnant Sprague-Dawley rats were used in this study. On gestational day 14 (GD 14), five rats received RUPP surgery to induce preeclampsia. US radiofrequency data were acquired for QUS analysis on GD18. On GD19, animals were sacrificed and dissected to acquire placental tissue samples. The cell nuclear diameter in each anatomical layer of the placenta was measured to compare with regional effective scatterer diameter (ESD) values.</p><p><strong>Results: </strong>ESD measurements obtained using in vivo QUS imaging correlated well (R<sup>2</sup> = 0.58, p = 3.8e-6) with cell nucleus diameter measurements from microscopy images. RUPP placentas had significantly smaller junctional zones compared to control placentas (p = 0.013). The average ESD in RUPP placentas was 1.0 µm smaller compared to control placentas (p = 0.040). This decrease in ESD in RUPP placentas is consistent with the decreased size of the junctional zone, which, in comparison to the labyrinth zone and chorionic plate, has larger cell nuclei (p = 3.3e-21 and p = 9.5e-27, respectively) and larger ESD (p = 5.6e-4 and p = 4.5e-8, respectively).</p><p><strong>Conclusion: </strong>These results demonstrate the potential of QUS as a non-invasive tool for detecting critical changes in placental microstructure, improving maternal and fetal outcomes by enabling earlier diagnosis and more timely therapeutic interventions in preeclampsia.</p>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":" ","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Quantitative Ultrasound-Based Characterization of Placental Tissue Microstructure in a Rat Model of Preeclampsia.\",\"authors\":\"Andrew C Markel, Cameron Hoerig, Allan K N de Alencar, Kenneth F Swan, Alexander D Gleed, Lili Shi, Gabriella Pridjian, Jonathan Mamou, Carolyn L Bayer\",\"doi\":\"10.1016/j.ultrasmedbio.2025.04.017\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Quantitative ultrasound (QUS) methods were applied to detect changes in placental microstructure in the reduced uterine perfusion pressure (RUPP) model of preeclampsia in rats. Preeclampsia is a life-threatening pregnancy disorder associated with abnormal placental development that is inadequately treated and managed.</p><p><strong>Methods: </strong>Nine timed-pregnant Sprague-Dawley rats were used in this study. On gestational day 14 (GD 14), five rats received RUPP surgery to induce preeclampsia. US radiofrequency data were acquired for QUS analysis on GD18. On GD19, animals were sacrificed and dissected to acquire placental tissue samples. The cell nuclear diameter in each anatomical layer of the placenta was measured to compare with regional effective scatterer diameter (ESD) values.</p><p><strong>Results: </strong>ESD measurements obtained using in vivo QUS imaging correlated well (R<sup>2</sup> = 0.58, p = 3.8e-6) with cell nucleus diameter measurements from microscopy images. RUPP placentas had significantly smaller junctional zones compared to control placentas (p = 0.013). The average ESD in RUPP placentas was 1.0 µm smaller compared to control placentas (p = 0.040). This decrease in ESD in RUPP placentas is consistent with the decreased size of the junctional zone, which, in comparison to the labyrinth zone and chorionic plate, has larger cell nuclei (p = 3.3e-21 and p = 9.5e-27, respectively) and larger ESD (p = 5.6e-4 and p = 4.5e-8, respectively).</p><p><strong>Conclusion: </strong>These results demonstrate the potential of QUS as a non-invasive tool for detecting critical changes in placental microstructure, improving maternal and fetal outcomes by enabling earlier diagnosis and more timely therapeutic interventions in preeclampsia.</p>\",\"PeriodicalId\":49399,\"journal\":{\"name\":\"Ultrasound in Medicine and Biology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-05-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ultrasound in Medicine and Biology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.ultrasmedbio.2025.04.017\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ACOUSTICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ultrasound in Medicine and Biology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.ultrasmedbio.2025.04.017","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ACOUSTICS","Score":null,"Total":0}
Quantitative Ultrasound-Based Characterization of Placental Tissue Microstructure in a Rat Model of Preeclampsia.
Objective: Quantitative ultrasound (QUS) methods were applied to detect changes in placental microstructure in the reduced uterine perfusion pressure (RUPP) model of preeclampsia in rats. Preeclampsia is a life-threatening pregnancy disorder associated with abnormal placental development that is inadequately treated and managed.
Methods: Nine timed-pregnant Sprague-Dawley rats were used in this study. On gestational day 14 (GD 14), five rats received RUPP surgery to induce preeclampsia. US radiofrequency data were acquired for QUS analysis on GD18. On GD19, animals were sacrificed and dissected to acquire placental tissue samples. The cell nuclear diameter in each anatomical layer of the placenta was measured to compare with regional effective scatterer diameter (ESD) values.
Results: ESD measurements obtained using in vivo QUS imaging correlated well (R2 = 0.58, p = 3.8e-6) with cell nucleus diameter measurements from microscopy images. RUPP placentas had significantly smaller junctional zones compared to control placentas (p = 0.013). The average ESD in RUPP placentas was 1.0 µm smaller compared to control placentas (p = 0.040). This decrease in ESD in RUPP placentas is consistent with the decreased size of the junctional zone, which, in comparison to the labyrinth zone and chorionic plate, has larger cell nuclei (p = 3.3e-21 and p = 9.5e-27, respectively) and larger ESD (p = 5.6e-4 and p = 4.5e-8, respectively).
Conclusion: These results demonstrate the potential of QUS as a non-invasive tool for detecting critical changes in placental microstructure, improving maternal and fetal outcomes by enabling earlier diagnosis and more timely therapeutic interventions in preeclampsia.
期刊介绍：
Ultrasound in Medicine and Biology is the official journal of the World Federation for Ultrasound in Medicine and Biology. The journal publishes original contributions that demonstrate a novel application of an existing ultrasound technology in clinical diagnostic, interventional and therapeutic applications, new and improved clinical techniques, the physics, engineering and technology of ultrasound in medicine and biology, and the interactions between ultrasound and biological systems, including bioeffects. Papers that simply utilize standard diagnostic ultrasound as a measuring tool will be considered out of scope. Extended critical reviews of subjects of contemporary interest in the field are also published, in addition to occasional editorial articles, clinical and technical notes, book reviews, letters to the editor and a calendar of forthcoming meetings. It is the aim of the journal fully to meet the information and publication requirements of the clinicians, scientists, engineers and other professionals who constitute the biomedical ultrasonic community.
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