旋转转录因子NtWHY1通过直接靶向烟草NtCBTS正向调节烟草类胶膜二萜的生物合成。

IF 3.6 2区 生物学 Q1 PLANT SCIENCES
Niu Zhai, Xiao Wei, Qingxia Zheng, Hui Zhang, Yalong Xu, Guoyun Xu, Leyu Zhang, Pingping Liu, Lifeng Jin, Jianfeng Zhang, Huina Zhou
{"title":"旋转转录因子NtWHY1通过直接靶向烟草NtCBTS正向调节烟草类胶膜二萜的生物合成。","authors":"Niu Zhai, Xiao Wei, Qingxia Zheng, Hui Zhang, Yalong Xu, Guoyun Xu, Leyu Zhang, Pingping Liu, Lifeng Jin, Jianfeng Zhang, Huina Zhou","doi":"10.1111/ppl.70280","DOIUrl":null,"url":null,"abstract":"<p><p>Cembranoid diterpenoids, as crucial secondary metabolites in tobacco, play significant physiological roles and exhibit notable biological activities, while the transcriptional regulators governing their biosynthesis remain largely unexplored. A whirly transcription factor NtWHY1 is screened out by DNA pull down using the promoter of NtCBTS (cembratrien-ol synthase), a known key gene in the pathway of cembranoid diterpenoid biosynthesis. Further experiments revealed that NtWHY1 encodes a protein with dual localization in chloroplasts and the nucleus, and it is highly transcriptionally active in tobacco's glandular trichomes and leaves. The expression level of NtWHY1 is positively correlated with the expression level of NtCBTS, as well as the products of α-cembrenediol (α-CBD) and β-cembrenediol (β-CBD), two main cembranoid diterpenoids in tobacco. We also proved that NtWHY1 can directly bind to the promoter region of NtCBTS, with evidence from chromatin immunoprecipitation (ChIP), dual-Luciferase (Dual-LUC) and electrophoretic mobility shift (EMSA) assays. Furthermore, ChIP assays have revealed that NtWHY1 silencing is correlated with reduced H3K9 acetylation and increased H3K27 methylation levels within the promoter region of NtCBTS. Collectively, our results elucidate a novel regulatory role of NtWHY1 in the biosynthesis of cembranoid diterpenoids, thereby advancing our understanding of plant secondary metabolism.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70280"},"PeriodicalIF":3.6000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Whirly Transcription Factor NtWHY1 Positively Regulates the Biosynthesis of Cembranoid Diterpenoids by Directly Targeting NtCBTS in Tobacco.\",\"authors\":\"Niu Zhai, Xiao Wei, Qingxia Zheng, Hui Zhang, Yalong Xu, Guoyun Xu, Leyu Zhang, Pingping Liu, Lifeng Jin, Jianfeng Zhang, Huina Zhou\",\"doi\":\"10.1111/ppl.70280\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Cembranoid diterpenoids, as crucial secondary metabolites in tobacco, play significant physiological roles and exhibit notable biological activities, while the transcriptional regulators governing their biosynthesis remain largely unexplored. A whirly transcription factor NtWHY1 is screened out by DNA pull down using the promoter of NtCBTS (cembratrien-ol synthase), a known key gene in the pathway of cembranoid diterpenoid biosynthesis. Further experiments revealed that NtWHY1 encodes a protein with dual localization in chloroplasts and the nucleus, and it is highly transcriptionally active in tobacco's glandular trichomes and leaves. The expression level of NtWHY1 is positively correlated with the expression level of NtCBTS, as well as the products of α-cembrenediol (α-CBD) and β-cembrenediol (β-CBD), two main cembranoid diterpenoids in tobacco. We also proved that NtWHY1 can directly bind to the promoter region of NtCBTS, with evidence from chromatin immunoprecipitation (ChIP), dual-Luciferase (Dual-LUC) and electrophoretic mobility shift (EMSA) assays. Furthermore, ChIP assays have revealed that NtWHY1 silencing is correlated with reduced H3K9 acetylation and increased H3K27 methylation levels within the promoter region of NtCBTS. Collectively, our results elucidate a novel regulatory role of NtWHY1 in the biosynthesis of cembranoid diterpenoids, thereby advancing our understanding of plant secondary metabolism.</p>\",\"PeriodicalId\":20164,\"journal\":{\"name\":\"Physiologia plantarum\",\"volume\":\"177 3\",\"pages\":\"e70280\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2025-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Physiologia plantarum\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1111/ppl.70280\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiologia plantarum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/ppl.70280","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

类Cembranoid二萜是烟草中重要的次生代谢产物,具有重要的生理作用和显著的生物活性,但调控其生物合成的转录调控因子仍未得到充分的研究。螺旋转录因子NtWHY1是通过DNA下拉,利用NtCBTS (embratrien-ol synthase)启动子筛选出来的,NtCBTS (embratrien-ol synthase)是已知的类膜二萜生物合成途径的关键基因。进一步的实验表明,NtWHY1编码一种在叶绿体和细胞核中具有双定位的蛋白,在烟草腺毛和叶片中具有高度的转录活性。NtWHY1的表达水平与NtCBTS以及烟草中两种主要的类膜二萜α-烯二醇(α-CBD)和β-烯二醇(β-CBD)的产物表达水平呈正相关。我们还通过染色质免疫沉淀(ChIP)、双荧光素酶(Dual-LUC)和电泳迁移转移(EMSA)实验证明了NtWHY1可以直接结合NtCBTS的启动子区域。此外,ChIP分析显示,NtWHY1沉默与NtCBTS启动子区域H3K9乙酰化降低和H3K27甲基化水平升高相关。总之,我们的研究结果阐明了NtWHY1在类膜二萜生物合成中的新调控作用,从而促进了我们对植物次生代谢的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Whirly Transcription Factor NtWHY1 Positively Regulates the Biosynthesis of Cembranoid Diterpenoids by Directly Targeting NtCBTS in Tobacco.

Cembranoid diterpenoids, as crucial secondary metabolites in tobacco, play significant physiological roles and exhibit notable biological activities, while the transcriptional regulators governing their biosynthesis remain largely unexplored. A whirly transcription factor NtWHY1 is screened out by DNA pull down using the promoter of NtCBTS (cembratrien-ol synthase), a known key gene in the pathway of cembranoid diterpenoid biosynthesis. Further experiments revealed that NtWHY1 encodes a protein with dual localization in chloroplasts and the nucleus, and it is highly transcriptionally active in tobacco's glandular trichomes and leaves. The expression level of NtWHY1 is positively correlated with the expression level of NtCBTS, as well as the products of α-cembrenediol (α-CBD) and β-cembrenediol (β-CBD), two main cembranoid diterpenoids in tobacco. We also proved that NtWHY1 can directly bind to the promoter region of NtCBTS, with evidence from chromatin immunoprecipitation (ChIP), dual-Luciferase (Dual-LUC) and electrophoretic mobility shift (EMSA) assays. Furthermore, ChIP assays have revealed that NtWHY1 silencing is correlated with reduced H3K9 acetylation and increased H3K27 methylation levels within the promoter region of NtCBTS. Collectively, our results elucidate a novel regulatory role of NtWHY1 in the biosynthesis of cembranoid diterpenoids, thereby advancing our understanding of plant secondary metabolism.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信