{"title":"旋转转录因子NtWHY1通过直接靶向烟草NtCBTS正向调节烟草类胶膜二萜的生物合成。","authors":"Niu Zhai, Xiao Wei, Qingxia Zheng, Hui Zhang, Yalong Xu, Guoyun Xu, Leyu Zhang, Pingping Liu, Lifeng Jin, Jianfeng Zhang, Huina Zhou","doi":"10.1111/ppl.70280","DOIUrl":null,"url":null,"abstract":"<p><p>Cembranoid diterpenoids, as crucial secondary metabolites in tobacco, play significant physiological roles and exhibit notable biological activities, while the transcriptional regulators governing their biosynthesis remain largely unexplored. A whirly transcription factor NtWHY1 is screened out by DNA pull down using the promoter of NtCBTS (cembratrien-ol synthase), a known key gene in the pathway of cembranoid diterpenoid biosynthesis. Further experiments revealed that NtWHY1 encodes a protein with dual localization in chloroplasts and the nucleus, and it is highly transcriptionally active in tobacco's glandular trichomes and leaves. The expression level of NtWHY1 is positively correlated with the expression level of NtCBTS, as well as the products of α-cembrenediol (α-CBD) and β-cembrenediol (β-CBD), two main cembranoid diterpenoids in tobacco. We also proved that NtWHY1 can directly bind to the promoter region of NtCBTS, with evidence from chromatin immunoprecipitation (ChIP), dual-Luciferase (Dual-LUC) and electrophoretic mobility shift (EMSA) assays. Furthermore, ChIP assays have revealed that NtWHY1 silencing is correlated with reduced H3K9 acetylation and increased H3K27 methylation levels within the promoter region of NtCBTS. Collectively, our results elucidate a novel regulatory role of NtWHY1 in the biosynthesis of cembranoid diterpenoids, thereby advancing our understanding of plant secondary metabolism.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70280"},"PeriodicalIF":3.6000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Whirly Transcription Factor NtWHY1 Positively Regulates the Biosynthesis of Cembranoid Diterpenoids by Directly Targeting NtCBTS in Tobacco.\",\"authors\":\"Niu Zhai, Xiao Wei, Qingxia Zheng, Hui Zhang, Yalong Xu, Guoyun Xu, Leyu Zhang, Pingping Liu, Lifeng Jin, Jianfeng Zhang, Huina Zhou\",\"doi\":\"10.1111/ppl.70280\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Cembranoid diterpenoids, as crucial secondary metabolites in tobacco, play significant physiological roles and exhibit notable biological activities, while the transcriptional regulators governing their biosynthesis remain largely unexplored. A whirly transcription factor NtWHY1 is screened out by DNA pull down using the promoter of NtCBTS (cembratrien-ol synthase), a known key gene in the pathway of cembranoid diterpenoid biosynthesis. Further experiments revealed that NtWHY1 encodes a protein with dual localization in chloroplasts and the nucleus, and it is highly transcriptionally active in tobacco's glandular trichomes and leaves. The expression level of NtWHY1 is positively correlated with the expression level of NtCBTS, as well as the products of α-cembrenediol (α-CBD) and β-cembrenediol (β-CBD), two main cembranoid diterpenoids in tobacco. We also proved that NtWHY1 can directly bind to the promoter region of NtCBTS, with evidence from chromatin immunoprecipitation (ChIP), dual-Luciferase (Dual-LUC) and electrophoretic mobility shift (EMSA) assays. Furthermore, ChIP assays have revealed that NtWHY1 silencing is correlated with reduced H3K9 acetylation and increased H3K27 methylation levels within the promoter region of NtCBTS. Collectively, our results elucidate a novel regulatory role of NtWHY1 in the biosynthesis of cembranoid diterpenoids, thereby advancing our understanding of plant secondary metabolism.</p>\",\"PeriodicalId\":20164,\"journal\":{\"name\":\"Physiologia plantarum\",\"volume\":\"177 3\",\"pages\":\"e70280\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2025-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Physiologia plantarum\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1111/ppl.70280\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiologia plantarum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/ppl.70280","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Whirly Transcription Factor NtWHY1 Positively Regulates the Biosynthesis of Cembranoid Diterpenoids by Directly Targeting NtCBTS in Tobacco.
Cembranoid diterpenoids, as crucial secondary metabolites in tobacco, play significant physiological roles and exhibit notable biological activities, while the transcriptional regulators governing their biosynthesis remain largely unexplored. A whirly transcription factor NtWHY1 is screened out by DNA pull down using the promoter of NtCBTS (cembratrien-ol synthase), a known key gene in the pathway of cembranoid diterpenoid biosynthesis. Further experiments revealed that NtWHY1 encodes a protein with dual localization in chloroplasts and the nucleus, and it is highly transcriptionally active in tobacco's glandular trichomes and leaves. The expression level of NtWHY1 is positively correlated with the expression level of NtCBTS, as well as the products of α-cembrenediol (α-CBD) and β-cembrenediol (β-CBD), two main cembranoid diterpenoids in tobacco. We also proved that NtWHY1 can directly bind to the promoter region of NtCBTS, with evidence from chromatin immunoprecipitation (ChIP), dual-Luciferase (Dual-LUC) and electrophoretic mobility shift (EMSA) assays. Furthermore, ChIP assays have revealed that NtWHY1 silencing is correlated with reduced H3K9 acetylation and increased H3K27 methylation levels within the promoter region of NtCBTS. Collectively, our results elucidate a novel regulatory role of NtWHY1 in the biosynthesis of cembranoid diterpenoids, thereby advancing our understanding of plant secondary metabolism.
期刊介绍:
Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.