巴西卫生机构使用者口服液丙型肝炎自检的可接受性和可用性：一项685名参与者的横断面研究

IF 3 4区医学 Q2 INFECTIOUS DISEASES

Brazilian Journal of Infectious Diseases Pub Date : 2025-05-23 DOI:10.1016/j.bjid.2025.104544

Hugo Perazzo , Cristiane Villela-Nogueira , Maria K. Gomes , Andre Daher , Cristiane Siqueira-do-Valle , Ketiuce Zukeram , Ana Cristina G. Ferreira , Karen Cristine Tonini , Elton Carlos de Almeida , Sandra W. Cardoso , Beatriz Grinsztejn , Valdilea G. Veloso

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引用次数: 0

摘要

HCV自检（HCV Self-Testing， HCVST）可用于HCV检测。我们的目的是评估巴西初级保健系统卫生机构用户口服液HCVST的可接受性/可用性和重新阅读/重新测试协议。材料和方法：本横断面研究邀请了2022年7月4日至2022年9月30日期间使用初级保健系统（PCS）的18-79岁连续人群。专业使用的OraQuick®HCV快速抗体测试被用作HCVST原型。根据循序渐进的视频和书面/图片说明进行口腔液HCVST。可用性通过观察到的错误和记录的医疗工作者（HCW）的帮助需求来评估。HCVST后，由HCW使用相同的检测试剂盒进行第二次HCV检测。评估复读和复测的一致性（Cohen’s kappa, κ）。评估测试后参与者的观点。结果685名参与者中，女性占74.5%；年龄中位数为52岁[IQR 39-61]岁，其中52.5%受教育年限≤10年)。观察到的主要错误[%(95%CI)]是不正确的样品采集[32.8%(29.4-36.5)]和错误地将测试装置放置在试管中[15.0%(12.6-17.9)]。总共有35.6% （95% CI 32.1-39.3）的参与者在HCVST的至少一个步骤中需要帮助。复读和复测协议分别为95.2% （κ = 0.56）和99.7% (κ = 0.67；N = 626，排除无效试验)。在HCVST后，93%的人感到安全，99%的人愿意再次进行测试，99%的人会推荐HCVST。大多数参与者认为HCVST体验简单（73%）或非常简单（24%）。结论口服液HCVST在巴西的PCS用户中是可行的，并且被广泛接受。HCVST可作为扩大HCV测试的替代方案。

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Acceptability and usability of oral fluid HCV self-testing among health-facility users from Brazil: a cross-sectional study of 685 participants

Introduction and objectives

HCV Self-Testing (HCVST) can be used to uptake HCV testing. We aimed to evaluate the acceptability/usability and re-reading/re-testing agreement of oral fluid HCVST among health-facility users in the Primary Care Systemin Brazil.

Materials and methods

Consecutive people aged 18‒79 years using the Primary Care System (PCS) from 04-July-2022 to 30-September-2022 were invited for this cross-sectional study. The professional use OraQuick® HCV Rapid Antibody Test was used as a HCVST prototype. Oral fluid HCVST was performed relying on a step-by-step video and written/pictorial instructions. Usability was assessed by observed errors and documented need of assistance by a Healthcare Worker (HCW). After HCVST, a second HCV test was performed by the HCW using the same test-kit. Re-reading and re-testing concordances were evaluated (Cohen’s kappa, κ). Post-testing participant’s perspectives were assessed.

Results

685 participants (74.5% female; median age = 52 [IQR 39‒61] years, 52.5% with schooling ≤ 10 years) were included. Major observed errors [%(95%CI)] were incorrect sample collection [32.8% (29.4‒36.5)] and wrong placing the test device in the tube [15.0% (12.6‒17.9)]. A total of 35.6% (95% CI 32.1‒39.3) of participants needed assistance in at least one step of HCVST. Re-reading and re-testing agreements were 95.2% (κ = 0.56) and 99.7% (κ = 0.67; n = 626 excluding invalid tests), respectively. After HCVST, 93% felt safe, 99% would be willing to test again, and 99% would recommend HCVST. Most participants rated the HCVST experience as easy (73%) or very easy (24%).

Conclusion

Oral-fluid HCVST was feasible and well-accepted among users of the PCS in Brazil. HCVST can be an alternative to scale-up HCV testing.

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来源期刊

Brazilian Journal of Infectious Diseases 医学-传染病学

CiteScore

5.50

自引率

0.00%

发文量

925

审稿时长

41 days

期刊介绍： The Brazilian Journal of Infectious Diseases is the official publication of the Brazilian Society of Infectious Diseases (SBI). It aims to publish relevant articles in the broadest sense on all aspects of microbiology, infectious diseases and immune response to infectious agents. The BJID is a bimonthly publication and one of the most influential journals in its field in Brazil and Latin America with a high impact factor, since its inception it has garnered a growing share of the publishing market.