美墨边境墨西卡利社区获得性尿路感染临床分离株PMQR和ESBL基因共存

IF 3 4区医学 Q2 INFECTIOUS DISEASES

Brazilian Journal of Infectious Diseases Pub Date : 2025-05-23 DOI:10.1016/j.bjid.2025.104554

Dolores A. Marquez-Salazar , Ricardo Delgadillo-Valles , Gerson N. Hernandez-Acevedo , Edwin Barrios-Villa , Raquel Muñiz-Salazar , Gilberto Lopez-Valencia , Paulina Haro , Enrique Trasviña-Muñoz , Rafael Martinez-Miranda , Jonathan Arauz-Cabrera

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引用次数: 0

摘要

大肠杆菌是一种机会致病菌，也是社区获得性尿路感染（ca - uti）的主要原因。大肠杆菌可携带多种遗传耐药决定因素，如广谱β-内酰胺酶（ESBL）和质粒介导的喹诺酮耐药（PMQR）基因，使β-内酰胺类和喹诺酮类药物治疗uti的经验复杂化。本研究的目的是对墨西哥墨西卡利CA-UTI大肠杆菌分离株的ESBL和PMQR基因进行特征分析。用MALDI-TOF法鉴定菌株，用Vitek 2法测定菌株产esbl和药敏。采用PCR检测ESBL和PMQR，并进行系统分型。遗传多样性采用肠杆菌重复基因间一致聚合酶链反应（ERIC-PCR）测定。从ca - uti中分离出89株大肠杆菌。所有患者均对环丙沙星、头孢曲松和头孢噻肟耐药，而对碳青霉烯类和头孢他啶/阿维巴坦敏感。所有分离株均检测出ESBL基因阳性，其中以blaCTX−M（98.9%）最为普遍。5株PMQR基因检测呈阴性；剩下的85.3%显示aac（6′）-lb-cr。95.5%的人发现ESBL和PMQR基因共存。多倍体发育组以B2组居多（74.2%）。该研究提供了有价值的区域数据，强调了由于导致CA-UTI的大肠杆菌中ESBL和PMQR基因的高流行率导致的公共卫生问题，这些基因与多药耐药性有关。发现了遗传多样性，表明群落中存在多种耐药菌株来源。这些发现强调需要进行监测和控制，以限制耐药大肠杆菌在当地和全球的传播。数据摘要作者确认在文章中或通过补充数据文件提供了所有支持数据、代码和协议。

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Coexistence of PMQR and ESBL genes among clinical Escherichia coli isolates from community-acquired UTI in Mexicali, on the US-Mexico border

Escherichia coli is an opportunistic pathogen and a leading cause of Community-Acquired Urinary Tract Infections (CA-UTIs). E. coli can harbor multiple genetic resistant determinants, such as Extended Spectrum Beta-lactamases (ESBL) and Plasmid-Mediated Quinolone Resistance (PMQR) genes, complicating the empirical treatment of UTIs with β-lactams and quinolones. The aim of his study was to characterize ESBL and PMQR genes among E. coli isolates from CA-UTI in Mexicali, Mexico Isolates were collected from January to December 2023. Identification was performed by MALDI-TOF, and ESBL-producing determination and antimicrobial susceptibility by Vitek 2. Detection of ESBL and PMQR, and phylotyping were performed by PCR. Genetic diversity was determined by Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR). Eighty-nine E. coli isolates were collected from CA-UTIs. All exhibited resistance to ciprofloxacin, ceftriaxone, and cefotaxime, while being susceptible to carbapenems and ceftazidime/avibactam. All isolates tested positive for an ESBL gene, with bla_CTX−_M (98.9 %) being the most prevalent. Five isolates tested negative for PMQR genes; the remaining showed aac(6′)-lb-cr present in 85.3 %. Coexistence between ESBL and PMQR genes was noted in 95.5 %. Most prevalent plylogenetic group was group B2 (74.2 %). This study provides valuable regional data, highlighting a public health problem due to the high prevalence of ESBL and PMQR genes in E. coli responsible for CA-UTI, which are linked to multidrug resistance. Genetic diversity was found, suggesting multiple sources of resistant strains in the community. These findings underline the need for surveillance and control to limit the spread of resistant E. coli, locally and globally.

Data summary

The authors confirm all supporting data, code, and protocols have been provided within the article or through supplementary data files.

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来源期刊

Brazilian Journal of Infectious Diseases 医学-传染病学

CiteScore

5.50

自引率

0.00%

发文量

925

审稿时长

41 days

期刊介绍： The Brazilian Journal of Infectious Diseases is the official publication of the Brazilian Society of Infectious Diseases (SBI). It aims to publish relevant articles in the broadest sense on all aspects of microbiology, infectious diseases and immune response to infectious agents. The BJID is a bimonthly publication and one of the most influential journals in its field in Brazil and Latin America with a high impact factor, since its inception it has garnered a growing share of the publishing market.