Yujie Lian , Yimin Hou , Qiujuan Ma , Guojiang Mao , Shuqi Hou , Yijie Ma , Ning Cui , Ruxue Xia
{"title":"高尔基定位近红外荧光探针识别半胱氨酸","authors":"Yujie Lian , Yimin Hou , Qiujuan Ma , Guojiang Mao , Shuqi Hou , Yijie Ma , Ning Cui , Ruxue Xia","doi":"10.1016/j.jphotochem.2025.116512","DOIUrl":null,"url":null,"abstract":"<div><div>Relevant studies have demonstrated that abnormal concentrations of cysteine (Cys) will directly affect normal biological processes within cells, leading to various diseases. As one of the important intracellular organelles, the Golgi apparatus (GA) serves as the primary site for protein synthesis. Cysteine is involved in protein formation and metabolism and other activities in cells, so its concentration is directly related to the proper function of the GA. Therefore, the development of a highly selective and sensitive method to detect the concentration of cysteine in the Golgi apparatus has important biomedical significance for diagnosing diseases. In this study, a naphthalimide-dicyanoisophorone conjugate was utilized as the fluorescent unit, benzenesulfonamide as the Golgi-localizing group, and acrylate as the recognition moiety to construct a Golgi-targeted near-infrared probe for Cys detection. When Cys was absent, the intramolecular charge transfer (ICT) effect was suppressed, resulting in minimal fluorescence emission from the probe. However, upon the addition of Cys, the ICT process was restored, inducing an enhancement in the NIR fluorescence emitted by the probe. Within the level scope from 0.2 μM to 10 μM, the fluorescence intensity of the probe demonstrated a linear correlation with the level of Cys. Furthermore, the limit of detection was determined as 0.024 μM. The fluorescent probe for Cys demonstrated excellent selectivity, superior sensitivity, rapid response time, and a broad pH working scope. Besides, this probe exhibited minimal cytotoxicity and was successfully applied for the detection of Cys in the GA of live cells. Furthermore, the probe was also effective in detecting cysteine in zebrafish and mice.</div></div>","PeriodicalId":16782,"journal":{"name":"Journal of Photochemistry and Photobiology A-chemistry","volume":"468 ","pages":"Article 116512"},"PeriodicalIF":4.7000,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Golgi-located near-infrared fluorescent probe for recognizing cysteine\",\"authors\":\"Yujie Lian , Yimin Hou , Qiujuan Ma , Guojiang Mao , Shuqi Hou , Yijie Ma , Ning Cui , Ruxue Xia\",\"doi\":\"10.1016/j.jphotochem.2025.116512\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Relevant studies have demonstrated that abnormal concentrations of cysteine (Cys) will directly affect normal biological processes within cells, leading to various diseases. As one of the important intracellular organelles, the Golgi apparatus (GA) serves as the primary site for protein synthesis. Cysteine is involved in protein formation and metabolism and other activities in cells, so its concentration is directly related to the proper function of the GA. Therefore, the development of a highly selective and sensitive method to detect the concentration of cysteine in the Golgi apparatus has important biomedical significance for diagnosing diseases. In this study, a naphthalimide-dicyanoisophorone conjugate was utilized as the fluorescent unit, benzenesulfonamide as the Golgi-localizing group, and acrylate as the recognition moiety to construct a Golgi-targeted near-infrared probe for Cys detection. When Cys was absent, the intramolecular charge transfer (ICT) effect was suppressed, resulting in minimal fluorescence emission from the probe. However, upon the addition of Cys, the ICT process was restored, inducing an enhancement in the NIR fluorescence emitted by the probe. Within the level scope from 0.2 μM to 10 μM, the fluorescence intensity of the probe demonstrated a linear correlation with the level of Cys. Furthermore, the limit of detection was determined as 0.024 μM. The fluorescent probe for Cys demonstrated excellent selectivity, superior sensitivity, rapid response time, and a broad pH working scope. Besides, this probe exhibited minimal cytotoxicity and was successfully applied for the detection of Cys in the GA of live cells. Furthermore, the probe was also effective in detecting cysteine in zebrafish and mice.</div></div>\",\"PeriodicalId\":16782,\"journal\":{\"name\":\"Journal of Photochemistry and Photobiology A-chemistry\",\"volume\":\"468 \",\"pages\":\"Article 116512\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2025-05-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Photochemistry and Photobiology A-chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1010603025002527\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Photochemistry and Photobiology A-chemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1010603025002527","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
Golgi-located near-infrared fluorescent probe for recognizing cysteine
Relevant studies have demonstrated that abnormal concentrations of cysteine (Cys) will directly affect normal biological processes within cells, leading to various diseases. As one of the important intracellular organelles, the Golgi apparatus (GA) serves as the primary site for protein synthesis. Cysteine is involved in protein formation and metabolism and other activities in cells, so its concentration is directly related to the proper function of the GA. Therefore, the development of a highly selective and sensitive method to detect the concentration of cysteine in the Golgi apparatus has important biomedical significance for diagnosing diseases. In this study, a naphthalimide-dicyanoisophorone conjugate was utilized as the fluorescent unit, benzenesulfonamide as the Golgi-localizing group, and acrylate as the recognition moiety to construct a Golgi-targeted near-infrared probe for Cys detection. When Cys was absent, the intramolecular charge transfer (ICT) effect was suppressed, resulting in minimal fluorescence emission from the probe. However, upon the addition of Cys, the ICT process was restored, inducing an enhancement in the NIR fluorescence emitted by the probe. Within the level scope from 0.2 μM to 10 μM, the fluorescence intensity of the probe demonstrated a linear correlation with the level of Cys. Furthermore, the limit of detection was determined as 0.024 μM. The fluorescent probe for Cys demonstrated excellent selectivity, superior sensitivity, rapid response time, and a broad pH working scope. Besides, this probe exhibited minimal cytotoxicity and was successfully applied for the detection of Cys in the GA of live cells. Furthermore, the probe was also effective in detecting cysteine in zebrafish and mice.
期刊介绍:
JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds.
All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor).
The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.