Edmilson F de Oliveira-Filho, Simon Franz Müller, Ianei O Carneiro, Otávio V de Carvalho, Alejandro Alfaro-Alarcón, Sebastian Brünink, Fagner D'ambroso Fernandes, Murilo H Anzolini Cassiano, Celia Pedroso, Felix Lehmann, Wendy K Jo, Andrés Moreira-Soto, Carlos Brites, Eduardo M Netto, Luiz E Ristow, Rita de Cassia Carvalho Maia, Fernanda S Flores Vogel, Nadia R de Almeida, Elisabeth Müller, Carlos R Franke, Jan Felix Drexler
{"title":"家猫乙型肝炎病毒的古老起源和全球传播。","authors":"Edmilson F de Oliveira-Filho, Simon Franz Müller, Ianei O Carneiro, Otávio V de Carvalho, Alejandro Alfaro-Alarcón, Sebastian Brünink, Fagner D'ambroso Fernandes, Murilo H Anzolini Cassiano, Celia Pedroso, Felix Lehmann, Wendy K Jo, Andrés Moreira-Soto, Carlos Brites, Eduardo M Netto, Luiz E Ristow, Rita de Cassia Carvalho Maia, Fernanda S Flores Vogel, Nadia R de Almeida, Elisabeth Müller, Carlos R Franke, Jan Felix Drexler","doi":"10.1093/ve/veaf025","DOIUrl":null,"url":null,"abstract":"<p><p>Mammalian hepadnaviruses have likely been evolving alongside their hosts for millions of years. Domestic cat HBV (DCHBV) has been detected in cats from several countries, but its genealogy, epidemiology, and host range remain unclear. Besides DCHBV, the only hepadnavirus identified among carnivores is the ringtail HBV (RtHBV). Because there is a gap in the felid fossil record of approximately 5-7 million years between the late Oligocene and the early Miocene, carnivore-derived viruses might help to shed light on Felidae evolution. Here, we screened 2260 sera and 154 paraffin-embedded liver samples from cats and 2123 sera from dogs sampled in Europe and South and Central America between 2018 and 2020 by PCR for DCHBV. We identified DCHBV genotype A (GtA) in 0.6% (7/1,195; 95% CI, 0.2-1.2) of cats sampled in Germany, France, Croatia, and Bulgaria and a genetically divergent DCHBV genotype B (GtB; 10.8% genomic sequence distance) in 0.2% of cats (2/1,065; 95% CI, 0.0-0.7) from Brazil. The detection rates of the two genotypes did not differ significantly (Fisher, <i>P</i> = .19). Viral loads ranged from 4 × 10<sup>1</sup>-6 × 10<sup>6</sup> for DCHBV GtA to 5-7 × 10<sup>3</sup> for DCHBV GtB DNA copies per milliliter of serum. None of the cat livers or dog sera tested positive by PCR. Immunoglobulin G against the DCHBV core antigen (anti-DCHBc) was detected in 8/504 cat sera (1.6%; 95% CI, 0.7-3.1), without significant variation between countries (χ<sup>2</sup>, <i>P</i> = .17), and in none of 180 dog sera by indirect immunofluorescence assay (IFA). Neither IFA (Fisher, <i>P</i> = .11; <i>n</i> = 311) nor PCR (Fisher, <i>P</i> = .63; <i>n</i> = 699) positivity was significantly associated with increased liver enzymes in cats, respectively. Coevolutionary reconciliations of virus and host phylogenies and Bayesian hypothesis testing suggested evolutionary origins of DCHBV during the Miocene, ∼8-17 million years ago (mya) from ancestral carnivores, consistent with long-term evolution. The long-term association of DCHBV with felines aids in elucidating orthohepadnaviral infection patterns and felid genealogy.</p>","PeriodicalId":56026,"journal":{"name":"Virus Evolution","volume":"11 1","pages":"veaf025"},"PeriodicalIF":5.5000,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091157/pdf/","citationCount":"0","resultStr":"{\"title\":\"Ancient origins and global spread of domestic cat hepatitis B virus.\",\"authors\":\"Edmilson F de Oliveira-Filho, Simon Franz Müller, Ianei O Carneiro, Otávio V de Carvalho, Alejandro Alfaro-Alarcón, Sebastian Brünink, Fagner D'ambroso Fernandes, Murilo H Anzolini Cassiano, Celia Pedroso, Felix Lehmann, Wendy K Jo, Andrés Moreira-Soto, Carlos Brites, Eduardo M Netto, Luiz E Ristow, Rita de Cassia Carvalho Maia, Fernanda S Flores Vogel, Nadia R de Almeida, Elisabeth Müller, Carlos R Franke, Jan Felix Drexler\",\"doi\":\"10.1093/ve/veaf025\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Mammalian hepadnaviruses have likely been evolving alongside their hosts for millions of years. Domestic cat HBV (DCHBV) has been detected in cats from several countries, but its genealogy, epidemiology, and host range remain unclear. Besides DCHBV, the only hepadnavirus identified among carnivores is the ringtail HBV (RtHBV). Because there is a gap in the felid fossil record of approximately 5-7 million years between the late Oligocene and the early Miocene, carnivore-derived viruses might help to shed light on Felidae evolution. Here, we screened 2260 sera and 154 paraffin-embedded liver samples from cats and 2123 sera from dogs sampled in Europe and South and Central America between 2018 and 2020 by PCR for DCHBV. We identified DCHBV genotype A (GtA) in 0.6% (7/1,195; 95% CI, 0.2-1.2) of cats sampled in Germany, France, Croatia, and Bulgaria and a genetically divergent DCHBV genotype B (GtB; 10.8% genomic sequence distance) in 0.2% of cats (2/1,065; 95% CI, 0.0-0.7) from Brazil. The detection rates of the two genotypes did not differ significantly (Fisher, <i>P</i> = .19). Viral loads ranged from 4 × 10<sup>1</sup>-6 × 10<sup>6</sup> for DCHBV GtA to 5-7 × 10<sup>3</sup> for DCHBV GtB DNA copies per milliliter of serum. None of the cat livers or dog sera tested positive by PCR. Immunoglobulin G against the DCHBV core antigen (anti-DCHBc) was detected in 8/504 cat sera (1.6%; 95% CI, 0.7-3.1), without significant variation between countries (χ<sup>2</sup>, <i>P</i> = .17), and in none of 180 dog sera by indirect immunofluorescence assay (IFA). Neither IFA (Fisher, <i>P</i> = .11; <i>n</i> = 311) nor PCR (Fisher, <i>P</i> = .63; <i>n</i> = 699) positivity was significantly associated with increased liver enzymes in cats, respectively. Coevolutionary reconciliations of virus and host phylogenies and Bayesian hypothesis testing suggested evolutionary origins of DCHBV during the Miocene, ∼8-17 million years ago (mya) from ancestral carnivores, consistent with long-term evolution. The long-term association of DCHBV with felines aids in elucidating orthohepadnaviral infection patterns and felid genealogy.</p>\",\"PeriodicalId\":56026,\"journal\":{\"name\":\"Virus Evolution\",\"volume\":\"11 1\",\"pages\":\"veaf025\"},\"PeriodicalIF\":5.5000,\"publicationDate\":\"2025-04-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091157/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Virus Evolution\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1093/ve/veaf025\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"VIROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Virus Evolution","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1093/ve/veaf025","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"VIROLOGY","Score":null,"Total":0}
Ancient origins and global spread of domestic cat hepatitis B virus.
Mammalian hepadnaviruses have likely been evolving alongside their hosts for millions of years. Domestic cat HBV (DCHBV) has been detected in cats from several countries, but its genealogy, epidemiology, and host range remain unclear. Besides DCHBV, the only hepadnavirus identified among carnivores is the ringtail HBV (RtHBV). Because there is a gap in the felid fossil record of approximately 5-7 million years between the late Oligocene and the early Miocene, carnivore-derived viruses might help to shed light on Felidae evolution. Here, we screened 2260 sera and 154 paraffin-embedded liver samples from cats and 2123 sera from dogs sampled in Europe and South and Central America between 2018 and 2020 by PCR for DCHBV. We identified DCHBV genotype A (GtA) in 0.6% (7/1,195; 95% CI, 0.2-1.2) of cats sampled in Germany, France, Croatia, and Bulgaria and a genetically divergent DCHBV genotype B (GtB; 10.8% genomic sequence distance) in 0.2% of cats (2/1,065; 95% CI, 0.0-0.7) from Brazil. The detection rates of the two genotypes did not differ significantly (Fisher, P = .19). Viral loads ranged from 4 × 101-6 × 106 for DCHBV GtA to 5-7 × 103 for DCHBV GtB DNA copies per milliliter of serum. None of the cat livers or dog sera tested positive by PCR. Immunoglobulin G against the DCHBV core antigen (anti-DCHBc) was detected in 8/504 cat sera (1.6%; 95% CI, 0.7-3.1), without significant variation between countries (χ2, P = .17), and in none of 180 dog sera by indirect immunofluorescence assay (IFA). Neither IFA (Fisher, P = .11; n = 311) nor PCR (Fisher, P = .63; n = 699) positivity was significantly associated with increased liver enzymes in cats, respectively. Coevolutionary reconciliations of virus and host phylogenies and Bayesian hypothesis testing suggested evolutionary origins of DCHBV during the Miocene, ∼8-17 million years ago (mya) from ancestral carnivores, consistent with long-term evolution. The long-term association of DCHBV with felines aids in elucidating orthohepadnaviral infection patterns and felid genealogy.
期刊介绍：
Virus Evolution is a new Open Access journal focusing on the long-term evolution of viruses, viruses as a model system for studying evolutionary processes, viral molecular epidemiology and environmental virology.
The aim of the journal is to provide a forum for original research papers, reviews, commentaries and a venue for in-depth discussion on the topics relevant to virus evolution.
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