利用多路扩展门场效应晶体管生物传感器实现个性化免疫治疗药物监测。

IF 8.3 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY
Small Science Pub Date : 2025-02-03 eCollection Date: 2025-05-01 DOI:10.1002/smsc.202400515
Trang-Anh Nguyen-Le, Christin Neuber, Isli Cela, Željko Janićijević, Liliana Rodrigues Loureiro, Lydia Hoffmann, Anja Feldmann, Michael Bachmann, Larysa Baraban
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引用次数: 0

摘要

癌症患者治疗方案的选择和优化迫切需要个性化。便携式医疗点电子生物传感器作为一种开创性的解决方案出现,有助于在精确肿瘤学中做出更好的决策。在这项研究中,展示了扩展门场效应晶体管(EG-FET)生物传感器的创新应用,用于监测体内免疫治疗药物的浓度和药代动力学。互补正电子发射断层扫描和小鼠放射性生物分布研究验证了eeg - fet测量结果。本文还引入了一种新的间接检测格式,用于检测适配器嵌合抗原受体t细胞治疗模型中的靶模块(TMs),有效地解决了当前电位测量的局限性。在药代动力学评估中,eeg - fet生物传感器的性能与标准放射性测量结果一致,揭示了小尺寸单链片段变量衍生的TMs(15分钟)和大尺寸igg4衍生的TMs(14小时)的不同寿命。有利的是,eeg - fet传感器表现出卓越的灵敏度,满足免疫治疗药物监测的要求,而无需复杂的放射性标记,这是必不可少的。在这些有希望的发现中,提倡探索下一代电子生物传感器作为治疗监测工具。凭借其成本、尺寸和响应时间的优势,这些生物传感器在推进个性化肿瘤学方面具有巨大的潜力,超越了文献中通常强调的传统诊断角色。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Toward Personalized Immunotherapeutic Drug Monitoring with Multiplexed Extended-Gate Field-Effect-Transistor Biosensors.

The selection and optimization of therapies for cancer patients urgently need personalization. Portable point-of-care electronic biosensors emerge as a groundbreaking solution contributing to better decision-making in precision oncology. In this study, the innovative use of extended-gate field-effect-transistor (EG-FET) biosensors is showcased for monitoring the concentration and pharmacokinetics of immunotherapeutic drugs in vivo. Complementary positron emission tomography and radioactivity biodistribution studies in mice validate the EG-FET measurements. Herein, a novel indirect assay format is also introduced for detecting target modules (TMs) in an adapter chimeric antigen receptor T-cell therapy model, effectively addressing the current limitations of potentiometric measurements. In pharmacokinetic evaluations, the EG-FET biosensor performance aligns with standard radioactivity measurements, revealing the distinct lifespans of small-sized single-chain-fragment-variable-derived TMs (15 min) and larger IgG4-derived TMs (14 h). Advantageously, the EG-FET sensors exhibit exceptional sensitivity and fulfill the requirements for immunotherapeutic drug monitoring without complex radioactive labeling, which is indispensable. In these promising findings, the exploration of next-generation electronic biosensors as therapeutic monitoring tools is advocated for. With their cost, size, and response time advantages, these biosensors hold immense potential for advancing personalized oncology, transcending the conventional diagnostic roles typically highlighted in the literature.

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来源期刊
CiteScore
14.00
自引率
2.40%
发文量
0
期刊介绍: Small Science is a premium multidisciplinary open access journal dedicated to publishing impactful research from all areas of nanoscience and nanotechnology. It features interdisciplinary original research and focused review articles on relevant topics. The journal covers design, characterization, mechanism, technology, and application of micro-/nanoscale structures and systems in various fields including physics, chemistry, materials science, engineering, environmental science, life science, biology, and medicine. It welcomes innovative interdisciplinary research and its readership includes professionals from academia and industry in fields such as chemistry, physics, materials science, biology, engineering, and environmental and analytical science. Small Science is indexed and abstracted in CAS, DOAJ, Clarivate Analytics, ProQuest Central, Publicly Available Content Database, Science Database, SCOPUS, and Web of Science.
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