{"title":"利用多路扩展门场效应晶体管生物传感器实现个性化免疫治疗药物监测。","authors":"Trang-Anh Nguyen-Le, Christin Neuber, Isli Cela, Željko Janićijević, Liliana Rodrigues Loureiro, Lydia Hoffmann, Anja Feldmann, Michael Bachmann, Larysa Baraban","doi":"10.1002/smsc.202400515","DOIUrl":null,"url":null,"abstract":"<p><p>The selection and optimization of therapies for cancer patients urgently need personalization. Portable point-of-care electronic biosensors emerge as a groundbreaking solution contributing to better decision-making in precision oncology. In this study, the innovative use of extended-gate field-effect-transistor (EG-FET) biosensors is showcased for monitoring the concentration and pharmacokinetics of immunotherapeutic drugs in vivo. Complementary positron emission tomography and radioactivity biodistribution studies in mice validate the EG-FET measurements. Herein, a novel indirect assay format is also introduced for detecting target modules (TMs) in an adapter chimeric antigen receptor T-cell therapy model, effectively addressing the current limitations of potentiometric measurements. In pharmacokinetic evaluations, the EG-FET biosensor performance aligns with standard radioactivity measurements, revealing the distinct lifespans of small-sized single-chain-fragment-variable-derived TMs (15 min) and larger IgG4-derived TMs (14 h). Advantageously, the EG-FET sensors exhibit exceptional sensitivity and fulfill the requirements for immunotherapeutic drug monitoring without complex radioactive labeling, which is indispensable. In these promising findings, the exploration of next-generation electronic biosensors as therapeutic monitoring tools is advocated for. With their cost, size, and response time advantages, these biosensors hold immense potential for advancing personalized oncology, transcending the conventional diagnostic roles typically highlighted in the literature.</p>","PeriodicalId":29791,"journal":{"name":"Small Science","volume":"5 5","pages":"2400515"},"PeriodicalIF":8.3000,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12087767/pdf/","citationCount":"0","resultStr":"{\"title\":\"Toward Personalized Immunotherapeutic Drug Monitoring with Multiplexed Extended-Gate Field-Effect-Transistor Biosensors.\",\"authors\":\"Trang-Anh Nguyen-Le, Christin Neuber, Isli Cela, Željko Janićijević, Liliana Rodrigues Loureiro, Lydia Hoffmann, Anja Feldmann, Michael Bachmann, Larysa Baraban\",\"doi\":\"10.1002/smsc.202400515\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The selection and optimization of therapies for cancer patients urgently need personalization. Portable point-of-care electronic biosensors emerge as a groundbreaking solution contributing to better decision-making in precision oncology. In this study, the innovative use of extended-gate field-effect-transistor (EG-FET) biosensors is showcased for monitoring the concentration and pharmacokinetics of immunotherapeutic drugs in vivo. Complementary positron emission tomography and radioactivity biodistribution studies in mice validate the EG-FET measurements. Herein, a novel indirect assay format is also introduced for detecting target modules (TMs) in an adapter chimeric antigen receptor T-cell therapy model, effectively addressing the current limitations of potentiometric measurements. In pharmacokinetic evaluations, the EG-FET biosensor performance aligns with standard radioactivity measurements, revealing the distinct lifespans of small-sized single-chain-fragment-variable-derived TMs (15 min) and larger IgG4-derived TMs (14 h). Advantageously, the EG-FET sensors exhibit exceptional sensitivity and fulfill the requirements for immunotherapeutic drug monitoring without complex radioactive labeling, which is indispensable. In these promising findings, the exploration of next-generation electronic biosensors as therapeutic monitoring tools is advocated for. With their cost, size, and response time advantages, these biosensors hold immense potential for advancing personalized oncology, transcending the conventional diagnostic roles typically highlighted in the literature.</p>\",\"PeriodicalId\":29791,\"journal\":{\"name\":\"Small Science\",\"volume\":\"5 5\",\"pages\":\"2400515\"},\"PeriodicalIF\":8.3000,\"publicationDate\":\"2025-02-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12087767/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Small Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/smsc.202400515\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/5/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"MATERIALS SCIENCE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Small Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/smsc.202400515","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
Toward Personalized Immunotherapeutic Drug Monitoring with Multiplexed Extended-Gate Field-Effect-Transistor Biosensors.
The selection and optimization of therapies for cancer patients urgently need personalization. Portable point-of-care electronic biosensors emerge as a groundbreaking solution contributing to better decision-making in precision oncology. In this study, the innovative use of extended-gate field-effect-transistor (EG-FET) biosensors is showcased for monitoring the concentration and pharmacokinetics of immunotherapeutic drugs in vivo. Complementary positron emission tomography and radioactivity biodistribution studies in mice validate the EG-FET measurements. Herein, a novel indirect assay format is also introduced for detecting target modules (TMs) in an adapter chimeric antigen receptor T-cell therapy model, effectively addressing the current limitations of potentiometric measurements. In pharmacokinetic evaluations, the EG-FET biosensor performance aligns with standard radioactivity measurements, revealing the distinct lifespans of small-sized single-chain-fragment-variable-derived TMs (15 min) and larger IgG4-derived TMs (14 h). Advantageously, the EG-FET sensors exhibit exceptional sensitivity and fulfill the requirements for immunotherapeutic drug monitoring without complex radioactive labeling, which is indispensable. In these promising findings, the exploration of next-generation electronic biosensors as therapeutic monitoring tools is advocated for. With their cost, size, and response time advantages, these biosensors hold immense potential for advancing personalized oncology, transcending the conventional diagnostic roles typically highlighted in the literature.
期刊介绍:
Small Science is a premium multidisciplinary open access journal dedicated to publishing impactful research from all areas of nanoscience and nanotechnology. It features interdisciplinary original research and focused review articles on relevant topics. The journal covers design, characterization, mechanism, technology, and application of micro-/nanoscale structures and systems in various fields including physics, chemistry, materials science, engineering, environmental science, life science, biology, and medicine. It welcomes innovative interdisciplinary research and its readership includes professionals from academia and industry in fields such as chemistry, physics, materials science, biology, engineering, and environmental and analytical science. Small Science is indexed and abstracted in CAS, DOAJ, Clarivate Analytics, ProQuest Central, Publicly Available Content Database, Science Database, SCOPUS, and Web of Science.