结直肠癌浸润性生物膜中富含核梭杆菌。

IF 9.2 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jessica Queen, Zam Cing, Hana Minsky, Asmita Nandi, Taylor Southward, Jacqueline Ferri, Madison McMann, Thevambiga Iyadorai, Jamuna Vadivelu, April Roslani, Mun Fai Loke, Jane Wanyiri, James R White, Julia L Drewes, Cynthia L Sears
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引用次数: 0

摘要

核梭杆菌是一种口腔细菌,已知在结直肠肿瘤中定植,它被认为在癌症进展中起重要作用。最近的测序和表型研究揭示了核仁梭菌在亚种水平上的重要差异,但这些差异是否影响整体肿瘤生态,以及肿瘤发生本身,仍然知之甚少。在这项研究中,我们试图通过结合分子、空间和微生物学分析来表征结直肠癌患者肿瘤微生物组中的梭杆菌。与配对的正常组织相比,我们评估了肿瘤中核仁梭菌的相对丰度,并将丰度与临床和病理特征相关联。我们证明了核梭菌和最近发现的亚种动物进化枝2 (Fna C2)在具有生物膜的结肠肿瘤中的显著富集,突出了复杂的群落伙伴关系在这种重要生物发病机制中的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fusobacterium nucleatum is enriched in invasive biofilms in colorectal cancer.

Fusobacterium nucleatum is an oral bacterium known to colonize colorectal tumors, where it is thought to play an important role in cancer progression. Recent advances in sequencing and phenotyping of F. nucleatum have revealed important differences at the subspecies level, but whether these differences impact the overall tumor ecology, and tumorigenesis itself, remain poorly understood. In this study, we sought to characterize Fusobacteria in the tumor microbiome of a cohort of individuals with CRC through a combination of molecular, spatial, and microbiologic analyses. We assessed for relative abundance of F. nucleatum in tumors compared to paired normal tissue, and correlated abundance with clinical and pathological features. We demonstrate striking enrichment of F. nucleatum and the recently discovered subspecies animalis clade 2 (Fna C2) specifically in colon tumors that have biofilms, highlighting the importance of complex community partnerships in the pathogenesis of this important organism.

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来源期刊
npj Biofilms and Microbiomes
npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology
CiteScore
12.10
自引率
3.30%
发文量
91
审稿时长
9 weeks
期刊介绍: npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.
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