harpin诱导的烟草超敏反应需要壁相关激酶2。

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES
Jeremy B Held, Judith P Sinn, Brian H Kvitko, Timothy W McNellis
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引用次数: 0

摘要

Harpins是由许多革兰氏阴性植物致病菌分泌的蛋白质,当它们以纯化蛋白质的形式渗透到植物叶片中时,会刺激宿主细胞死亡防御反应——超敏反应(HR)。harpin的这种活性最早在烟草中被发现,并在浸润后12-24 h内表现出强烈而快速的HR活性。HrpN是火疫病病菌淀粉欧文菌的主要基因。我们发现了hrpn诱导的HR在烟草制品中的自然变异,并利用遗传作图和全基因组测序的散装分离分析确定了候选基因。病毒诱导的候选基因壁相关激酶2 (Wall-Associated Kinase 2, WAK2)基因沉默使HR对HrpN和HpaG(一种来自大豆细菌性脓疱病原菌柑橘黄单胞菌的harpin)的反应失效。甘氨酸。WAK2沉默也降低了harpin HrpZ对烟草野火病原菌丁香假单胞菌的毒力活性。烟。WAK2的自然破坏性突变与烟草制品无法装载harpin介导的HR相关。我们认为,预测的受体样激酶WAK2是烟草叶片中harpin蛋白浸润诱导的强HR所必需的,并且可能基于harpin识别介导对细菌病原体的抗性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Harpin-Induced Hypersensitive Reaction in Nicotiana tabacum Requires Wall-Associated Kinase 2.

Harpins are proteins secreted by many gram-negative, plant-pathogenic bacteria that stimulate the hypersensitive reaction (HR), a host cell death defence response, when infiltrated into plant leaves as purified proteins. This activity of harpins was first discovered in Nicotiana tabacum (tobacco), which manifests an especially strong and rapid harpin-activated HR that becomes evident within 12-24 h after infiltration. HrpN is the major harpin of the fire blight pathogen Erwinia amylovora. We discovered natural variation in the HrpN-induced HR among tobacco accessions and identified candidate genes using genetic mapping and bulked-segregant analysis with whole genome sequencing. Virus-induced gene silencing of candidate gene Wall-Associated Kinase 2 (WAK2) abrogated the HR in response to HrpN and HpaG, a harpin from the soybean bacterial pustule pathogen Xanthomonas citri pv. glycines. WAK2 silencing also compromised the avirulence activity of harpin HrpZ in the tobacco wildfire pathogen Pseudomonas syringae pv. tabaci. A natural, disruptive mutation in WAK2 correlated with the inability of tobacco accessions to mount the harpin-mediated HR. We conclude that the predicted receptor-like kinase WAK2 is required for the strong HR induced in tobacco leaves by harpin protein infiltration and can potentially mediate resistance to bacterial pathogens based on harpin recognition.

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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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