Clavibacter michiganensis Reframed: The Story of How the Genomics Era Made a New Face for an Old Enemy.

Objective: Bacterial wilt and canker of tomato caused by the gram-positive corynebacterial species Clavibacter michiganensis is an economically important disease threatening the tomato industry in both open-air and greenhouse productions around the world. The disease occurs in many countries, with a particular importance in regions characterised by high temperature and water scarcity. Management of bacterial canker has been a major problem since its original description in 1909. This is due in part to the seedborne nature of the pathogen, allowing the bacterium to be transmitted over long distances via infected seeds, as well as a lack of effective treatment to clean seeds. Detection of the pathogen from seeds is difficult due to high competition on culture media with diverse members of the seed-associated microbiota. Identification of the pathogen can also be difficult owing to the presence of different colony variants on culture media. In this review, we provide a historical perspective and an updated overview on the aetiology, epidemiology and management strategies of the bacterial canker disease. We also gathered recent molecular findings in the pathogenicity mechanisms and bioecology of C. michiganensis to boost management of the bacterial canker disease in the 21^st century tomato industry.

Taxonomy: Class: Actinobacteria; Order: Micrococcales; Family: Microbacteriaceae; Genus: Clavibacter; Species: Clavibacter michiganensis.

Disease symptoms: Interveinal leaf chlorosis leading to necrotic areas. Canker on stems and lateral branches of the plant. Discolouration of vascular and pith tissues to dark yellow or brown. Small and early ripened fruits or discolouration of the placenta from white to yellow in the interior part of the ripening fruits.

Host range: Tomato (Solanum lycopersicum) is the main host of the pathogen while natural infection has also been reported on eggplant, pepper and wild nightshade plants.

Synonyms (historical/non-preferred scientific names): Aplanobacter michiganensis; Pseudomonas michiganense; Pseudomonas michiganensis; Bacterium michiganense; Phytomonas michiganensis; Mycobacterium michiganense; Erwinia michiganensis (=michiganense); Corynebacterium michiganense; Corynebacterium michiganense pv. michiganense; Corynebacterium michiganense subsp. michiganense; Clavibacter michiganensis subsp. michiganensis.

Microbiological properties: The bacterium produces domed, round and shiny mucoid colonies on general culture media. Colonies are usually yellow-pigmented, while pink-pigmented strains are occasionally observed. Cells are gram-positive, aerobic, non-motile, non-spore-producing curved rods (coryneform).

Distribution: Present in all continents.

Phytosanitary categorization: EPPO A2 List no. 50, EU 2019/2072 RNQP Annex IV. See EPPO (https://gd.eppo.int/taxon/CORBMI/categorization) and CABI (https://www.cabidigitallibrary.org/doi/10.1079/cabicompendium.15338) databases for further country-specific categorisations. EPPO code: CORBMI.