persimilis Phytoseiulus(蜱螨目:蜱螨科)对病原菌phytoseiuli粉螨的免疫应答。

IF 1.8 2区 农林科学 Q2 ENTOMOLOGY
Yu Chen, Endong Wang, Xuenong Xu, Bo Zhang
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引用次数: 0

摘要

节肢动物的天然免疫系统是抵御病原体感染的重要防御系统。掠食性螨作为一种高度商品化的天敌,在规模化饲养过程中受到病原菌感染的威胁。然而,关于掠食性螨对病原体感染的免疫机制研究较少。为了探究persimilis是否对病原体具有天然的免疫应答,我们选择了典型的革兰氏阳性细菌Acaricomes phytoseiuli来研究致病对persimilis适应性的影响以及潜在的免疫相关基因。结果表明,紫花假单胞菌感染后,紫花假单胞菌的存活率、繁殖力和捕食率均显著降低。利用荧光原位杂交技术鉴定了persimilis的卵巢和肠道中富集的病原菌。为了进一步研究persimilis的天然免疫防御,我们分析了从转录组中选择的12个免疫基因的表达。结果表明,tok、HPB、DUOX和Peroxidase 4个基因的表达水平在病原菌感染1天后上调。而其他基因的表达在第1天和第3天均无显著差异。本研究对植物螨的天然免疫机制进行了初步探索,为植物螨进一步抗病奠定了理论基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The immune response of Phytoseiulus persimilis (Acari: Phytoseiidae) to the bacterial pathogen Acaricomes phytoseiuli.

The natural immune system of arthropods is an important defense against pathogen infection. Predatory mites, as a highly commercialized group of natural enemies, suffer the threat of pathogen infection during mass rearing processes. However, there is a lack of research on the immune mechanisms of predatory mites in response to pathogen infection. To explore whether the Phytoseiulus persimilis possesses a natural immune response to pathogen, we selected the typical Gram-positive bacterium Acaricomes phytoseiuli to examine the pathogenic impact on P. persimilis fitness and the potential immune-related genes. The results showed that the survival rate, fecundity and predation of P. persimilis were significantly reduced after infection with A. phytoseiuli. Furthermore, fluorescent in situ hybridization was used to identify the pathogen enriched in the ovary and intestinal tract of P. persimilis. To further investigate the natural immune defense of P. persimilis, we analyzed the expression of twelve immune genes selected from the transcriptome. The results showed that the expression levels of four genes, tok, HPB, DUOX, and Peroxidase, were upregulated after one day of pathogen infection. While the expression of other genes showed no significant differences in either the 1st day or 3rd day. Our study provide preliminary exploration of natural immune mechanism of phytoseiid mites, laying a theoretical foundation for further their disease resistance.

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来源期刊
CiteScore
3.90
自引率
9.10%
发文量
81
审稿时长
4-8 weeks
期刊介绍: Experimental and Applied Acarology publishes peer-reviewed original papers describing advances in basic and applied research on mites and ticks. Coverage encompasses all Acari, including those of environmental, agricultural, medical and veterinary importance, and all the ways in which they interact with other organisms (plants, arthropods and other animals). The subject matter draws upon a wide variety of disciplines, including evolutionary biology, ecology, epidemiology, physiology, biochemistry, toxicology, immunology, genetics, molecular biology and pest management sciences.
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