牛源性高致病性H5N1 B3.13病毒在猪中的致病性和传播性。

IF 7.5 2区 医学 Q1 IMMUNOLOGY
Emerging Microbes & Infections Pub Date : 2025-12-01 Epub Date: 2025-06-16 DOI:10.1080/22221751.2025.2509742
Taeyong Kwon, Jessie D Trujillo, Mariano Carossino, Heather M Machkovech, Konner Cool, Eu Lim Lyoo, Gagandeep Singh, Sujan Kafle, Shanmugasundaram Elango, Govindsamy Vediyappan, Wanting Wei, Nicholas Minor, Franco S Matias-Ferreyra, Igor Morozov, Natasha N Gaudreault, Udeni B R Balasuriya, Lisa E Hensley, Diego G Diel, Wenjun Ma, Thomas C Friedrich, Juergen A Richt
{"title":"牛源性高致病性H5N1 B3.13病毒在猪中的致病性和传播性。","authors":"Taeyong Kwon, Jessie D Trujillo, Mariano Carossino, Heather M Machkovech, Konner Cool, Eu Lim Lyoo, Gagandeep Singh, Sujan Kafle, Shanmugasundaram Elango, Govindsamy Vediyappan, Wanting Wei, Nicholas Minor, Franco S Matias-Ferreyra, Igor Morozov, Natasha N Gaudreault, Udeni B R Balasuriya, Lisa E Hensley, Diego G Diel, Wenjun Ma, Thomas C Friedrich, Juergen A Richt","doi":"10.1080/22221751.2025.2509742","DOIUrl":null,"url":null,"abstract":"<p><p>Since the first emergence of highly pathogenic avian influenza (HPAI) H5N1 viruses in dairy cattle, the virus has continued to spread, reaching 17 states and at least 1000 dairy herds in the United States. Subsequently, spillovers of the virus from dairy cattle to humans have been reported. Pigs are an important host in influenza ecology because they serve as a mixing vessel in which novel reassortant viruses with pandemic potential can be generated. Here, we show that oro-respiratory infection of pigs resulted in productive replication of a bovine-derived HPAI H5N1 B3.13 virus. Infectious virus and viral antigen were mainly identified in the lower respiratory tract of principal infected pigs, and sero-conversion was observed in most of the principal pigs at later time points, suggesting replication of the bovine-derived HPAI H5N1 B3.13 virus in pigs. In one animal, we detected the emergence of a mutation in the hemagglutinin (HA) previously associated with increased affinity for \"mammalian-type\" α2,6-linked sialic acid receptors, but this mutation did not reach majority consensus levels. Sentinel contact pigs remained sero-negative throughout the study, indicating lack of transmission. These results support that pigs are susceptible to a bovine-derived HPAI H5N1 B3.13 virus, but this virus did not replicate as robustly in pigs as swine-adapted influenza viruses.</p>","PeriodicalId":11602,"journal":{"name":"Emerging Microbes & Infections","volume":" ","pages":"2509742"},"PeriodicalIF":7.5000,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12172082/pdf/","citationCount":"0","resultStr":"{\"title\":\"Pathogenicity and transmissibility of bovine-derived HPAI H5N1 B3.13 virus in pigs.\",\"authors\":\"Taeyong Kwon, Jessie D Trujillo, Mariano Carossino, Heather M Machkovech, Konner Cool, Eu Lim Lyoo, Gagandeep Singh, Sujan Kafle, Shanmugasundaram Elango, Govindsamy Vediyappan, Wanting Wei, Nicholas Minor, Franco S Matias-Ferreyra, Igor Morozov, Natasha N Gaudreault, Udeni B R Balasuriya, Lisa E Hensley, Diego G Diel, Wenjun Ma, Thomas C Friedrich, Juergen A Richt\",\"doi\":\"10.1080/22221751.2025.2509742\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Since the first emergence of highly pathogenic avian influenza (HPAI) H5N1 viruses in dairy cattle, the virus has continued to spread, reaching 17 states and at least 1000 dairy herds in the United States. Subsequently, spillovers of the virus from dairy cattle to humans have been reported. Pigs are an important host in influenza ecology because they serve as a mixing vessel in which novel reassortant viruses with pandemic potential can be generated. Here, we show that oro-respiratory infection of pigs resulted in productive replication of a bovine-derived HPAI H5N1 B3.13 virus. Infectious virus and viral antigen were mainly identified in the lower respiratory tract of principal infected pigs, and sero-conversion was observed in most of the principal pigs at later time points, suggesting replication of the bovine-derived HPAI H5N1 B3.13 virus in pigs. In one animal, we detected the emergence of a mutation in the hemagglutinin (HA) previously associated with increased affinity for \\\"mammalian-type\\\" α2,6-linked sialic acid receptors, but this mutation did not reach majority consensus levels. Sentinel contact pigs remained sero-negative throughout the study, indicating lack of transmission. These results support that pigs are susceptible to a bovine-derived HPAI H5N1 B3.13 virus, but this virus did not replicate as robustly in pigs as swine-adapted influenza viruses.</p>\",\"PeriodicalId\":11602,\"journal\":{\"name\":\"Emerging Microbes & Infections\",\"volume\":\" \",\"pages\":\"2509742\"},\"PeriodicalIF\":7.5000,\"publicationDate\":\"2025-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12172082/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Emerging Microbes & Infections\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/22221751.2025.2509742\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/6/16 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Emerging Microbes & Infections","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/22221751.2025.2509742","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/16 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

自首次在奶牛中出现高致病性禽流感H5N1病毒以来,该病毒继续传播,在美国至少波及17个州和至少950个奶牛群。随后,报告了该病毒从奶牛向人类的溢出。猪是流感生态学中一个重要的储存库,因为它们可以作为一个混合容器,在其中可以产生具有大流行潜力的新型重组病毒。本研究表明,猪的口呼吸道感染导致牛源性高致病性H5N1 B3.13病毒的高效复制。感染病毒主要在主要感染猪的下呼吸道中发现,并且在较晚的时间点在大多数主要猪中观察到血清转化,提示牛源性高致病性H5N1 B3.13病毒在猪中复制有限。在一只动物中,我们检测到血凝素(HA)突变的出现,该突变先前与“哺乳动物型”α2,6-链唾液酸受体的亲和力增加有关,但该突变未达到多数共识水平。在整个研究过程中,哨点接触猪的血清呈阴性,表明没有传播。这些结果支持猪对牛源性高致病性H5N1 B3.13病毒易感,但该病毒在猪体内的复制不像水貂源性高致病性H5N1和猪适应型流感病毒那样强劲。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Pathogenicity and transmissibility of bovine-derived HPAI H5N1 B3.13 virus in pigs.

Pathogenicity and transmissibility of bovine-derived HPAI H5N1 B3.13 virus in pigs.

Pathogenicity and transmissibility of bovine-derived HPAI H5N1 B3.13 virus in pigs.

Pathogenicity and transmissibility of bovine-derived HPAI H5N1 B3.13 virus in pigs.

Since the first emergence of highly pathogenic avian influenza (HPAI) H5N1 viruses in dairy cattle, the virus has continued to spread, reaching 17 states and at least 1000 dairy herds in the United States. Subsequently, spillovers of the virus from dairy cattle to humans have been reported. Pigs are an important host in influenza ecology because they serve as a mixing vessel in which novel reassortant viruses with pandemic potential can be generated. Here, we show that oro-respiratory infection of pigs resulted in productive replication of a bovine-derived HPAI H5N1 B3.13 virus. Infectious virus and viral antigen were mainly identified in the lower respiratory tract of principal infected pigs, and sero-conversion was observed in most of the principal pigs at later time points, suggesting replication of the bovine-derived HPAI H5N1 B3.13 virus in pigs. In one animal, we detected the emergence of a mutation in the hemagglutinin (HA) previously associated with increased affinity for "mammalian-type" α2,6-linked sialic acid receptors, but this mutation did not reach majority consensus levels. Sentinel contact pigs remained sero-negative throughout the study, indicating lack of transmission. These results support that pigs are susceptible to a bovine-derived HPAI H5N1 B3.13 virus, but this virus did not replicate as robustly in pigs as swine-adapted influenza viruses.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Emerging Microbes & Infections
Emerging Microbes & Infections IMMUNOLOGY-MICROBIOLOGY
CiteScore
26.20
自引率
2.30%
发文量
276
审稿时长
20 weeks
期刊介绍: Emerging Microbes & Infections is a peer-reviewed, open-access journal dedicated to publishing research at the intersection of emerging immunology and microbiology viruses. The journal's mission is to share information on microbes and infections, particularly those gaining significance in both biological and clinical realms due to increased pathogenic frequency. Emerging Microbes & Infections is committed to bridging the scientific gap between developed and developing countries. This journal addresses topics of critical biological and clinical importance, including but not limited to: - Epidemic surveillance - Clinical manifestations - Diagnosis and management - Cellular and molecular pathogenesis - Innate and acquired immune responses between emerging microbes and their hosts - Drug discovery - Vaccine development research Emerging Microbes & Infections invites submissions of original research articles, review articles, letters, and commentaries, fostering a platform for the dissemination of impactful research in the field.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信