调查美国成年人血铅和镉与吸烟相关的DNA甲基化和死亡率之间的关系

IF 6.2 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Ecotoxicology and Environmental Safety Pub Date : 2025-05-22 DOI:10.1016/j.ecoenv.2025.118360

Yu-Wei Fang , Ching-Way Chen , Ta-Chen Su , Chikang Wang , Chien-Yu Lin

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引用次数: 0

摘要

铅和镉与DNA甲基化（DNAm）的改变和死亡率的增加有关。然而，与吸烟有关的脱氧核糖核酸在这些重金属与死亡率之间的关系中所起的作用仍不清楚。方法和结果我们分析了1999-2002年国家健康与营养调查（NHANES）中2110名50岁及以上参与者的数据，并将其与国家卫生统计中心（NCHS）的死亡率数据联系起来，随访至2019年。我们的研究检查了血铅和镉水平、DNA甲基化预测吸烟包年（DNAmPackYrs）和死亡率结果之间的关系。我们的分析发现，较高的自然对数（Ln）转化的铅和镉水平与Ln - dnampackyrs呈正相关，铅的百分比变化为11.34 % (P = 0.001)，镉的百分比变化为35.57 % （P <； 0.001）。两种重金属含量均高于第50百分位的参与者的DNAmPackYrs最高，P代表趋势<； 0.001。加权Cox回归分析显示，铅-铅和铅- dnampackyrs与所有死亡结果的风险升高有关，而镉-铅特异性预测全因死亡率。观察到铅和镉与全因死亡率之间存在显著的相互作用（相互作用P = 0.036）。此外，发现DNAmPackYrs与两种重金属之间的显著相互作用与全因死亡率有关(相互作用P = 0.001；相互作用P = 0.013)。结论：这项研究从具有全国代表性的美国老年人队列中得出，提供了强有力的证据，证明血铅和镉水平与吸烟相关的脱氧核糖核酸和死亡风险增加有关。此外，分析还揭示了这些金属对吸烟相关的dna的加性影响，以及对全因死亡率的协同影响。此外，吸烟诱导的脱氧核糖核酸可能在重金属暴露与死亡风险之间发挥中介作用。需要进一步的研究来调查这些关联的潜在机制。

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Investigating the associations of blood lead and cadmium with smoking-related DNA methylation and mortality among U.S. adults

Purpose

Lead and cadmium have been linked to alterations in DNA methylation (DNAm) and increased mortality. However, the role of smoking-related DNAm in the link between these heavy metals and mortality remains unclear.

Approach and results

We analyzed data from 2110 participants aged 50 and older from the 1999–2002 National Health and Nutrition Examination Survey (NHANES), linked to mortality data from the National Center for Health Statistics (NCHS) with follow-up through 2019. Our study examined the associations between blood lead and cadmium levels, DNA methylation-predicted pack years of smoking (DNAmPackYrs), and mortality outcomes. Our analysis found that higher natural logarithm (Ln)-transformed lead and cadmium levels were positively associated with ln-DNAmPackYrs, with a percent change of 11.34 % (P = 0.001) for lead and 35.57 % (P < 0.001) for cadmium. Participants with both heavy metals above the 50th percentile had the highest DNAmPackYrs, with P for trend < 0.001. Weighted Cox regression analysis demonstrated both ln-lead and ln-DNAmPackYrs were linked to an elevated risk of all mortality outcomes, while ln-cadmium specifically predicted all-cause mortality. A significant interaction between lead and cadmium in relation to all-cause mortality was observed (P for interaction = 0.036). Additionally, significant interactions between DNAmPackYrs and both heavy metals were found in relation to all-cause mortality (P for interaction = 0.001 for lead; P for interaction = 0.013 for cadmium).

Conclusions

Drawing from a nationally representative cohort of older U.S. adults, this study provides robust evidence linking blood lead and cadmium levels to smoking-related DNAm and increased mortality risk. Moreover, the analysis revealed additive effects of these metals on smoking-related DNAm, as well as synergistic impacts on all-cause mortality. Additionally, smoking-induced DNAm may play a role in mediating the connection between heavy metal exposure and mortality risk. Additional research is required to investigate the underlying mechanisms of these associations.

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来源期刊

Ecotoxicology and Environmental Safety 环境科学-毒理学

CiteScore

12.10

自引率

5.90%

发文量

1234

审稿时长

88 days

期刊介绍： Ecotoxicology and Environmental Safety is a multi-disciplinary journal that focuses on understanding the exposure and effects of environmental contamination on organisms including human health. The scope of the journal covers three main themes. The topics within these themes, indicated below, include (but are not limited to) the following: Ecotoxicology、Environmental Chemistry、Environmental Safety etc.