S.M. Ibrahim , S.A. Amer , G.E. Elshopakey , E.M. Younis , A.A. Abdel-Warith , A. Osman , A. Gouda , R. Reda , H.A. Mohammed , A.A.A. Abdel-Wareth , J. Lohakare , M.I.M. Darwish
{"title":"豇豆蛋白水解物对肉鸡生长性能、脂质、抗氧化状态、组织形态学以及caspase-3和肿瘤坏死因子-α表达的影响","authors":"S.M. Ibrahim , S.A. Amer , G.E. Elshopakey , E.M. Younis , A.A. Abdel-Warith , A. Osman , A. Gouda , R. Reda , H.A. Mohammed , A.A.A. Abdel-Wareth , J. Lohakare , M.I.M. Darwish","doi":"10.1016/j.livsci.2025.105722","DOIUrl":null,"url":null,"abstract":"<div><div>The impact of cowpea protein hydrolysate (CPH) dietary supplementation was assessed on the growth performance, blood hematology, liver histomorphology, antioxidant status, and inflammatory responses in broiler chickens. Five hundred 3-d-old broiler chickens (88.72 g ± 0.20) were assigned to 5 experimental treatments (10 replicates/treatment), the basal diet supplemented with 5 inclusion rates of CPH: 0, 2, 4, 6, or 8 g/kg. Throughout the experimental periods, broiler chickens fed diets supplemented with CPH showed no change in the growth rate. The 2 g CPH/kg treatment increased the red blood cell count, hemoglobin concentration, packed cell volume percentage, white blood cell count, and lymphocyte count (linear, <em>P</em> < 0.01; quadratic, <em>P</em> < 0.05). Uric acid concentration increased in the CPH-supplemented treatments (linear, <em>P</em> = 0.031). The inclusion of CPH reduced the total cholesterol (Linear, <em>P</em> = 0.03; quadratic, <em>P</em> = 0.02), the low-density lipoprotein concentrations (linear, <em>P</em> = 0.012), and the triglyceride concentrations (linear, <em>P</em> = 0.01). Dietary supplementation of CPH increased the total antioxidant capacity, catalase (linear, <em>P</em> < 0.001), and superoxide dismutase activity (linear, quadratic, <em>P</em> < 0.01). Supplementation of CPH showed a down-regulation of the expression of caspase-3 antibodies in the liver tissues (Linear, <em>P</em> < 0.01). Liver histoarchitecture was normal in all experimental treatments. It can be concluded that dietary cowpea protein hydrolysate can be used as a potential protein supplement in broiler chicken diets to improve antioxidant status and lower the blood lipids without affecting the broiler chickens’ growth rate.</div></div>","PeriodicalId":18152,"journal":{"name":"Livestock Science","volume":"297 ","pages":"Article 105722"},"PeriodicalIF":1.9000,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of cowpea protein hydrolysates on the growth performance, lipid profile, antioxidant status, tissue histomorphology, and expression of caspase-3 and tumor necrosis factor-α in broiler chickens\",\"authors\":\"S.M. Ibrahim , S.A. Amer , G.E. Elshopakey , E.M. Younis , A.A. Abdel-Warith , A. Osman , A. Gouda , R. Reda , H.A. Mohammed , A.A.A. Abdel-Wareth , J. Lohakare , M.I.M. Darwish\",\"doi\":\"10.1016/j.livsci.2025.105722\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The impact of cowpea protein hydrolysate (CPH) dietary supplementation was assessed on the growth performance, blood hematology, liver histomorphology, antioxidant status, and inflammatory responses in broiler chickens. Five hundred 3-d-old broiler chickens (88.72 g ± 0.20) were assigned to 5 experimental treatments (10 replicates/treatment), the basal diet supplemented with 5 inclusion rates of CPH: 0, 2, 4, 6, or 8 g/kg. Throughout the experimental periods, broiler chickens fed diets supplemented with CPH showed no change in the growth rate. The 2 g CPH/kg treatment increased the red blood cell count, hemoglobin concentration, packed cell volume percentage, white blood cell count, and lymphocyte count (linear, <em>P</em> < 0.01; quadratic, <em>P</em> < 0.05). Uric acid concentration increased in the CPH-supplemented treatments (linear, <em>P</em> = 0.031). The inclusion of CPH reduced the total cholesterol (Linear, <em>P</em> = 0.03; quadratic, <em>P</em> = 0.02), the low-density lipoprotein concentrations (linear, <em>P</em> = 0.012), and the triglyceride concentrations (linear, <em>P</em> = 0.01). Dietary supplementation of CPH increased the total antioxidant capacity, catalase (linear, <em>P</em> < 0.001), and superoxide dismutase activity (linear, quadratic, <em>P</em> < 0.01). Supplementation of CPH showed a down-regulation of the expression of caspase-3 antibodies in the liver tissues (Linear, <em>P</em> < 0.01). Liver histoarchitecture was normal in all experimental treatments. It can be concluded that dietary cowpea protein hydrolysate can be used as a potential protein supplement in broiler chicken diets to improve antioxidant status and lower the blood lipids without affecting the broiler chickens’ growth rate.</div></div>\",\"PeriodicalId\":18152,\"journal\":{\"name\":\"Livestock Science\",\"volume\":\"297 \",\"pages\":\"Article 105722\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2025-05-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Livestock Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S187114132500085X\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Livestock Science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S187114132500085X","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Effects of cowpea protein hydrolysates on the growth performance, lipid profile, antioxidant status, tissue histomorphology, and expression of caspase-3 and tumor necrosis factor-α in broiler chickens
The impact of cowpea protein hydrolysate (CPH) dietary supplementation was assessed on the growth performance, blood hematology, liver histomorphology, antioxidant status, and inflammatory responses in broiler chickens. Five hundred 3-d-old broiler chickens (88.72 g ± 0.20) were assigned to 5 experimental treatments (10 replicates/treatment), the basal diet supplemented with 5 inclusion rates of CPH: 0, 2, 4, 6, or 8 g/kg. Throughout the experimental periods, broiler chickens fed diets supplemented with CPH showed no change in the growth rate. The 2 g CPH/kg treatment increased the red blood cell count, hemoglobin concentration, packed cell volume percentage, white blood cell count, and lymphocyte count (linear, P < 0.01; quadratic, P < 0.05). Uric acid concentration increased in the CPH-supplemented treatments (linear, P = 0.031). The inclusion of CPH reduced the total cholesterol (Linear, P = 0.03; quadratic, P = 0.02), the low-density lipoprotein concentrations (linear, P = 0.012), and the triglyceride concentrations (linear, P = 0.01). Dietary supplementation of CPH increased the total antioxidant capacity, catalase (linear, P < 0.001), and superoxide dismutase activity (linear, quadratic, P < 0.01). Supplementation of CPH showed a down-regulation of the expression of caspase-3 antibodies in the liver tissues (Linear, P < 0.01). Liver histoarchitecture was normal in all experimental treatments. It can be concluded that dietary cowpea protein hydrolysate can be used as a potential protein supplement in broiler chicken diets to improve antioxidant status and lower the blood lipids without affecting the broiler chickens’ growth rate.
期刊介绍:
Livestock Science promotes the sound development of the livestock sector by publishing original, peer-reviewed research and review articles covering all aspects of this broad field. The journal welcomes submissions on the avant-garde areas of animal genetics, breeding, growth, reproduction, nutrition, physiology, and behaviour in addition to genetic resources, welfare, ethics, health, management and production systems. The high-quality content of this journal reflects the truly international nature of this broad area of research.