{"title":"定制微生物接种剂增强荒漠化草原根鞘功能，推动生态系统恢复","authors":"Qiqi Han, Weimeng Liu, Yingjie Jiang, Pengyuan Yang, Zixuan Wang, Ziyuan Yan, Zhuo Zhang, Rui Xu, Hui Tang, Guixia Liu","doi":"10.1007/s11104-025-07524-1","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Aims</h3><p>This study aimed to develop microbial inoculants composed of functional strains isolated from the rhizosheath of <i>Agropyron cristatum</i> (L.) Gaertn., a pioneer species in desertified grasslands, and to evaluate their effects on root exudates, rhizosheath properties, and microbial community composition. The ultimate goal was to enhance rhizosheath functionality and promote plant growth.</p><h3 data-test=\"abstract-sub-heading\">Methods</h3><p>Microbial inoculants were formulated by combining functional strains in various proportions. A short-term greenhouse experiment was conducted to assess changes in root exudates, including sugars, proteins, and organic acids, as well as key rhizosheath soil parameters: seedling height, soil weight, pH, electrical conductivity (EC), organic matter (OM), available phosphorus (AP), and available nitrogen (AN). Metagenomic sequencing was employed to analyze the microbial community composition and diversity in rhizosheath soil.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>Microbial inoculants significantly increased the concentrations of sugars, proteins, and tartaric acid in root exudates, lowered rhizosheath soil pH, and enhanced seedling height, EC, and soil nutrient content (OM, AP, and AN). Metagenomic sequencing revealed that inoculation altered the composition of the rhizosheath microbial community, increasing the relative abundance of bacterial phyla such as Actinobacteria, Proteobacteria, and Firmicutes, as well as the fungal phylum Ascomycota. Root exudate composition, particularly soluble sugars and proteins, was closely associated with microbial community shifts.</p><h3 data-test=\"abstract-sub-heading\">Conclusion</h3><p>Customized microbial inoculants significantly improved rhizosheath properties, reshaped microbial community composition, and promoted plant growth in <i>A. cristatum</i>. These findings provide valuable insights into the application of microbial inoculants for ecological restoration in desertified grasslands by improving soil health and enhancing plant productivity. </p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\n","PeriodicalId":20223,"journal":{"name":"Plant and Soil","volume":"32 1","pages":""},"PeriodicalIF":4.1000,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Customized microbial inoculants enhance rhizosheath functionality and drive ecosystem restoration in desertified grasslands\",\"authors\":\"Qiqi Han, Weimeng Liu, Yingjie Jiang, Pengyuan Yang, Zixuan Wang, Ziyuan Yan, Zhuo Zhang, Rui Xu, Hui Tang, Guixia Liu\",\"doi\":\"10.1007/s11104-025-07524-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Aims</h3><p>This study aimed to develop microbial inoculants composed of functional strains isolated from the rhizosheath of <i>Agropyron cristatum</i> (L.) Gaertn., a pioneer species in desertified grasslands, and to evaluate their effects on root exudates, rhizosheath properties, and microbial community composition. The ultimate goal was to enhance rhizosheath functionality and promote plant growth.</p><h3 data-test=\\\"abstract-sub-heading\\\">Methods</h3><p>Microbial inoculants were formulated by combining functional strains in various proportions. A short-term greenhouse experiment was conducted to assess changes in root exudates, including sugars, proteins, and organic acids, as well as key rhizosheath soil parameters: seedling height, soil weight, pH, electrical conductivity (EC), organic matter (OM), available phosphorus (AP), and available nitrogen (AN). Metagenomic sequencing was employed to analyze the microbial community composition and diversity in rhizosheath soil.</p><h3 data-test=\\\"abstract-sub-heading\\\">Results</h3><p>Microbial inoculants significantly increased the concentrations of sugars, proteins, and tartaric acid in root exudates, lowered rhizosheath soil pH, and enhanced seedling height, EC, and soil nutrient content (OM, AP, and AN). Metagenomic sequencing revealed that inoculation altered the composition of the rhizosheath microbial community, increasing the relative abundance of bacterial phyla such as Actinobacteria, Proteobacteria, and Firmicutes, as well as the fungal phylum Ascomycota. Root exudate composition, particularly soluble sugars and proteins, was closely associated with microbial community shifts.</p><h3 data-test=\\\"abstract-sub-heading\\\">Conclusion</h3><p>Customized microbial inoculants significantly improved rhizosheath properties, reshaped microbial community composition, and promoted plant growth in <i>A. cristatum</i>. These findings provide valuable insights into the application of microbial inoculants for ecological restoration in desertified grasslands by improving soil health and enhancing plant productivity. </p><h3 data-test=\\\"abstract-sub-heading\\\">Graphical Abstract</h3>\\n\",\"PeriodicalId\":20223,\"journal\":{\"name\":\"Plant and Soil\",\"volume\":\"32 1\",\"pages\":\"\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2025-05-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant and Soil\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s11104-025-07524-1\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant and Soil","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11104-025-07524-1","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
Customized microbial inoculants enhance rhizosheath functionality and drive ecosystem restoration in desertified grasslands
Aims
This study aimed to develop microbial inoculants composed of functional strains isolated from the rhizosheath of Agropyron cristatum (L.) Gaertn., a pioneer species in desertified grasslands, and to evaluate their effects on root exudates, rhizosheath properties, and microbial community composition. The ultimate goal was to enhance rhizosheath functionality and promote plant growth.
Methods
Microbial inoculants were formulated by combining functional strains in various proportions. A short-term greenhouse experiment was conducted to assess changes in root exudates, including sugars, proteins, and organic acids, as well as key rhizosheath soil parameters: seedling height, soil weight, pH, electrical conductivity (EC), organic matter (OM), available phosphorus (AP), and available nitrogen (AN). Metagenomic sequencing was employed to analyze the microbial community composition and diversity in rhizosheath soil.
Results
Microbial inoculants significantly increased the concentrations of sugars, proteins, and tartaric acid in root exudates, lowered rhizosheath soil pH, and enhanced seedling height, EC, and soil nutrient content (OM, AP, and AN). Metagenomic sequencing revealed that inoculation altered the composition of the rhizosheath microbial community, increasing the relative abundance of bacterial phyla such as Actinobacteria, Proteobacteria, and Firmicutes, as well as the fungal phylum Ascomycota. Root exudate composition, particularly soluble sugars and proteins, was closely associated with microbial community shifts.
Conclusion
Customized microbial inoculants significantly improved rhizosheath properties, reshaped microbial community composition, and promoted plant growth in A. cristatum. These findings provide valuable insights into the application of microbial inoculants for ecological restoration in desertified grasslands by improving soil health and enhancing plant productivity.
期刊介绍：
Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.
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