uORF-HsfA1a-WOX11模块控制水稻冠根发育。

IF 8.3 1区 生物学 Q1 PLANT SCIENCES
New Phytologist Pub Date : 2025-05-21 DOI:10.1111/nph.70214
Ting Zhang,Yimeng Xiang,Miaomiao Ye,Meng Yuan,Guoyong Xu,Dao-Xiu Zhou,Yu Zhao
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引用次数: 0

摘要

OsWOX11是水稻冠根发育的关键决定因子。然而,OsWOX11的过表达或下调都会导致多效性发育缺陷,包括侏儒症和产量降低。因此,为了平衡地下根系和空气器官的发育,有必要确保OsWOX11的最佳表达水平。OsHsfA1a通过直接结合其启动子内的热应激元件样元件激活OsWOX11表达。遗传证据表明,OsHsfA1a过表达或敲除转基因植株对OsHsfA1a转基因植株的冠根生长有表型影响。此外,在OsHsfA1a RNAi背景下,OsWOX11的表达增加可以部分补充缺陷的冠根表型。在OsHsfA1a的5'-未翻译区发现了一个uORF (uORFHsfA1a)。瞬时表达分析结合核糖体分析显示,uORFHsfA1a降低了OsHsfA1a mRNA的翻译效率。此外,HsfA1aP:uORFHsfA1a- hsfa1a - gfp植株表现出野生型冠根表型,而uORFHsfA1a基因敲除转基因植株表现出与OsWOX11过表达植株相似的冠根表型。这些发现表明,uORFHsfA1a通过抑制OsHsfA1a的翻译来微调冠根的发育,从而间接调节OsWOX11的转录水平。我们的研究展示了一种新的uORFHsfA1a-HsfA1a-WOX11调控模块,该模块协调转录和翻译控制以维持最佳的OsWOX11表达。这种机制保证了根和芽发育之间的平衡。重要的是,针对uORFHsfA1a调控元件提供了在不影响农艺性状的情况下构建强健根系结构的新策略,从而解决了谷物作物改良中的关键挑战。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The uORF-HsfA1a-WOX11 module controls crown root development in rice.
OsWOX11 is a key essential determinant of crown root development in rice. However, either overexpression or downregulation of OsWOX11 results in pleiotropic developmental defects, including dwarfism and reduced yield. Therefore, it is necessary to ensure an optimal level of OsWOX11 expression for balancing the subterranean root system and aerial organ development. OsHsfA1a activates OsWOX11 expression by directly binding to heat stress element-like elements within its promoter. Genetic evidence demonstrated that OsHsfA1a overexpressing or knockout transgenic plants phenocopied the crown root growth in OsWOX11 transgenic plants. Additionally, increased expression of OsWOX11 in OsHsfA1a RNAi background could partially complement the defective crown root phenotypes. A uORF (uORFHsfA1a) was identified within the 5'-untranslated region of OsHsfA1a. Transient expression assays coupled with ribosome profiling revealed that uORFHsfA1a attenuated the translation efficiency of OsHsfA1a mRNA. Furthermore, HsfA1aP:uORFHsfA1a-HsfA1a-GFP plants exhibited wild-type crown root phenotypes, whereas uORFHsfA1a knockout transgenic plants displayed similar crown root phenotypes to OsWOX11 overexpressing plants. These findings suggest that uORFHsfA1a fine-tunes the crown root development by repressing OsHsfA1a translation, thereby indirectly modulating OsWOX11 transcript levels. Our study demonstrated a novel uORFHsfA1a-HsfA1a-WOX11 regulatory module that coordinated transcriptional and translational control to maintain optimal OsWOX11 expression. This mechanism ensures the trade-off between root and shoot development. Importantly, targeting uORFHsfA1a regulatory elements provided a new strategy for engineering robust root system architecture without compromising agronomic traits, thereby addressing a critical challenge in cereal crop improvement.
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来源期刊
New Phytologist
New Phytologist 生物-植物科学
自引率
5.30%
发文量
728
期刊介绍: New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.
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