大规模人群饮食转变为素食对泰国污水抗生素耐药性和细菌组成的影响

IF 2.6 4区医学 Q3 INFECTIOUS DISEASES

Infection Genetics and Evolution Pub Date : 2025-05-17 DOI:10.1016/j.meegid.2025.105765

Pimlapas Leekitcharoenphon , Valeria Bortolaia , Viktor Karl Wilhelm Tornblom , Achiraya Siriphap , Orasa Suthienkul , Jednipit Borthong , Kaknokrat Chonsin , Frederik Duus Møller , Baptiste Avot , Saria Otani , Frank M. Aarestrup

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引用次数: 0

摘要

抗微生物药物耐药性（AMR）是全球优先面临的挑战之一。本研究通过宏基因组分析，探讨了2019年10月泰国年度素食节期间饮食改变对AMR细菌的影响。这项研究通过收集那空沙湾、素叻他尼和曼谷的城市污水，在节前、节中和节后，调查了从常规饮食到素食的10天转变的影响。此外，对北部城市个人的粪便样本进行了分析。使用散弹枪宏基因组测序，将样品与细菌、AMR基因和碳水化合物活性酶数据库进行比对。结果显示，在节日期间，三个城市的污水样本中AMR基因丰度发生了显著变化，碳水化合物代谢基因增加。细菌种类的组成和多样性也发生了显著变化，尤其是在北部城市。在素食节期间，所有地区的AMR基因的总丰度都有所增加。这项研究强调了泰国人群的素食饮食与抗生素耐药性增加之间的相关性。研究还表明，污水宏基因组分析可以有效评估饮食变化对细菌群落和抗菌素耐药性的影响，为公共卫生战略提供有价值的见解。

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Effect of large-scale population-based dietary change to vegetarianism on antimicrobial resistance and bacterial composition of sewage in Thailand

Antimicrobial resistance (AMR) is on one of the global priority challenges. This study explored the impact of diet alteration on AMR bacteria through metagenomic analysis during the annual vegetarian festival in Thailand in October 2019. The study investigated the effects of a 10-day shift from a regular to a vegetarian diet by collecting urban sewage from Nakhon Sawan, Surat Thani, and Bangkok before, during, and after the festival. Additionally, faecal samples from individuals in the northern city were analyzed. Using shotgun metagenomic sequencing, the samples were mapped against bacterial, AMR genes, and carbohydrate-active enzymes databases.

The results revealed significant changes in AMR gene abundance and increased carbohydrate metabolism genes in sewage samples from all three cities during the festival. There was also a notable shift in the composition and diversity of bacterial species, particularly in the northern city. The total abundance of AMR genes increased during the vegetarian festival across all locations. This study highlights the correlation between a population's vegetarian diet and increased AMR in Thailand. It also demonstrates that metagenomic analysis of sewage can effectively assess the impact of dietary changes on bacterial communities and AMR at a population level, providing valuable insights for public health strategies.

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来源期刊

Infection Genetics and Evolution 医学-传染病学

CiteScore

8.40

自引率

0.00%

发文量

215

审稿时长

82 days

期刊介绍： (aka Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases -- MEEGID) Infectious diseases constitute one of the main challenges to medical science in the coming century. The impressive development of molecular megatechnologies and of bioinformatics have greatly increased our knowledge of the evolution, transmission and pathogenicity of infectious diseases. Research has shown that host susceptibility to many infectious diseases has a genetic basis. Furthermore, much is now known on the molecular epidemiology, evolution and virulence of pathogenic agents, as well as their resistance to drugs, vaccines, and antibiotics. Equally, research on the genetics of disease vectors has greatly improved our understanding of their systematics, has increased our capacity to identify target populations for control or intervention, and has provided detailed information on the mechanisms of insecticide resistance. However, the genetics and evolutionary biology of hosts, pathogens and vectors have tended to develop as three separate fields of research. This artificial compartmentalisation is of concern due to our growing appreciation of the strong co-evolutionary interactions among hosts, pathogens and vectors. Infection, Genetics and Evolution and its companion congress [MEEGID](http://www.meegidconference.com/) (for Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases) are the main forum acting for the cross-fertilization between evolutionary science and biomedical research on infectious diseases. Infection, Genetics and Evolution is the only journal that welcomes articles dealing with the genetics and evolutionary biology of hosts, pathogens and vectors, and coevolution processes among them in relation to infection and disease manifestation. All infectious models enter the scope of the journal, including pathogens of humans, animals and plants, either parasites, fungi, bacteria, viruses or prions. The journal welcomes articles dealing with genetics, population genetics, genomics, postgenomics, gene expression, evolutionary biology, population dynamics, mathematical modeling and bioinformatics. We also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services .