MicroRNA-486:非小细胞肺癌诊断和肿瘤免疫微环境特征的双功能生物标志物

IF 2.1 4区 医学 Q3 GENETICS & HEREDITY
Jun Yu, Yi Shen, Yao Xu, Zhengyang Feng, Yuntian Shen, Yaqun Zhu, Jian Huan, Qiliang Peng
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引用次数: 0

摘要

背景:本研究通过综合分析方法评估microRNA-486 (miR-486)作为非小细胞肺癌(NSCLC)潜在生物标志物的临床意义和分子机制。方法:我们对主要生物医学数据库中自成立至2025年4月4日的诊断研究进行了系统检索和荟萃分析,然后进行了全面的生物信息学询问。利用STRING构建蛋白-蛋白相互作用(PPI)网络,鉴定miR-486调控的关键枢纽基因。枢纽基因验证采用TCGA数据集,免疫浸润分析采用TIMER2.0平台。结果:荟萃分析表明,miR-486无论是单独还是联合使用,都可能是检测NSCLC的有效生物标志物。随后,miR-486靶基因的功能富集分析突出了对NSCLC的发生和发展至关重要的重要本体术语和途径。PPI网络揭示了参与与NSCLC发病相关的多种基本途径的关键蛋白和模块。此外,鉴定的枢纽基因在癌组织与正常组织中的差异表达被验证,表明其潜在的诊断效用,而随后的生存分析通过与总生存的显着关联证实了它们的预后价值。值得注意的是,这些中心基因被发现与非小细胞肺癌的免疫浸润水平、免疫微环境评分和免疫相关蛋白显著相关。结论:这项双模式研究确立了miR-486作为非小细胞肺癌的多功能生物标志物,通过肿瘤微环境调节显示出诊断效用和免疫调节潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MicroRNA-486: a dual-function biomarker for diagnosis and tumor immune microenvironment characterization in non-small cell lung cancer.

Background: This investigation evaluates the clinical significance and molecular mechanisms of microRNA-486 (miR-486) as a potential biomarker in non-small cell lung cancer (NSCLC) through an integrative analytical approach.

Methods: We conducted systematic search and meta-analysis of diagnostic studies from major biomedical databases from inception through April 04, 2025, followed by comprehensive bioinformatics interrogation. Protein-protein interaction (PPI) networks were constructed using STRING to identify key hub genes regulated by miR-486. Validation of hub genes employed TCGA datasets, while immune infiltration analysis utilized TIMER2.0 platform.

Results: The meta-analysis indicated that miR-486, both individually and in combination, could be effective biomarkers for NSCLC detection. Afterwards, functional enrichment analyses of miR-486 target genes highlighted significant ontology terms and pathways crucial to the initiation and progression of NSCLC. PPI networks revealed key proteins and modules that participate in multiple essential pathways associated with NSCLC pathogenesis. Furthermore, the identified hub genes were validated for differential expression in cancerous versus normal tissues, suggesting their potential diagnostic utility, while subsequent survival analyses confirmed their prognostic value through significant associations with overall survival. Notably, these hub genes were found to be significantly associated with immune infiltration levels, immune microenvironment scores, and immune-related proteins in NSCLC.

Conclusions: This dual-modality investigation establishes miR-486 as a multi-functional biomarker in NSCLC, demonstrating both diagnostic utility and immunoregulatory potential through tumor microenvironment modulation.

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来源期刊
BMC Medical Genomics
BMC Medical Genomics 医学-遗传学
CiteScore
3.90
自引率
0.00%
发文量
243
审稿时长
3.5 months
期刊介绍: BMC Medical Genomics is an open access journal publishing original peer-reviewed research articles in all aspects of functional genomics, genome structure, genome-scale population genetics, epigenomics, proteomics, systems analysis, and pharmacogenomics in relation to human health and disease.
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