{"title":"生物有机肥通过调节向日葵转录组和根际微生物群落减轻向日葵寄生","authors":"Tengqi Xu, Jiao Xi, Yufan Wang, Yiqiong Zhang, Jihong Ke, Xiaomin Wei, Yanbing Lin","doi":"10.1007/s11104-025-07542-z","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Background and aims</h3><p><i>Orobanche cumana</i> Wallr. is a holoparasitic weed that poses an increasing threat to sunflower cultivation. However, the influence of bio-organic fertilizers on <i>O. cumana</i>, remains inadequately understood. This study investigates the relationship between sunflower microbial community and <i>O. cumana</i> parasitism, as well as the inhibitory mechanism of bio-organic fertilizers on <i>O. cumana</i>.</p><h3 data-test=\"abstract-sub-heading\">Methods</h3><p>Field investigation was conducted to study the response mechanisms of host microbial communities to parasitic plants using amplicon techniques. Pot experiment, the transcriptome was employed to investigate the mechanism by which bio-organic fertilizer inhibits <i>O. cumana</i> parasitism. Field experiments were conducted to explore changes in sunflower yield, quality and rhizosphere microecology.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>The results revealed rhizosphere bacterial community composition shifted notably with escalating parasite number. Bio-organic fertilizers application markedly upregulated the expression of <i>Mn-SOD</i>, <i>GST</i>, <i>XTH9</i>, <i>ACC01</i> and <i>NPR1</i> genes associated with plant resistance, and significantly increased the levels of the fruit ripening-related hormones ACC and tZTR. Application of bio-organic fertilizer remolded the rhizosphere microbial structure and significantly increased seed yield and crude protein content of sunflower. Upon applying bio-organic fertilizers, <i>O. cumana</i> roots exhibited decay-inducing fungal colonization, yet the isolated strain <i>Fusarium solani</i> L5 significantly reducing the number of parasites.</p><h3 data-test=\"abstract-sub-heading\">Conclusions</h3><p>Bio-organic fertilizers demonstrated efficacy in suppressing <i>O. cumana</i> parasitism while enhancing sunflower productivity and quality. The results enhance our understanding of bio-organic fertilizers for controlling parasitic plants, offering insights into overcoming agricultural challenges through rhizosphere microbial communities.</p>","PeriodicalId":20223,"journal":{"name":"Plant and Soil","volume":"131 1","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bio-organic fertilizer mitigates Orobanche cumana parasitism by regulating sunflower transcriptomes and rhizosphere microbial community\",\"authors\":\"Tengqi Xu, Jiao Xi, Yufan Wang, Yiqiong Zhang, Jihong Ke, Xiaomin Wei, Yanbing Lin\",\"doi\":\"10.1007/s11104-025-07542-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Background and aims</h3><p><i>Orobanche cumana</i> Wallr. is a holoparasitic weed that poses an increasing threat to sunflower cultivation. However, the influence of bio-organic fertilizers on <i>O. cumana</i>, remains inadequately understood. This study investigates the relationship between sunflower microbial community and <i>O. cumana</i> parasitism, as well as the inhibitory mechanism of bio-organic fertilizers on <i>O. cumana</i>.</p><h3 data-test=\\\"abstract-sub-heading\\\">Methods</h3><p>Field investigation was conducted to study the response mechanisms of host microbial communities to parasitic plants using amplicon techniques. Pot experiment, the transcriptome was employed to investigate the mechanism by which bio-organic fertilizer inhibits <i>O. cumana</i> parasitism. Field experiments were conducted to explore changes in sunflower yield, quality and rhizosphere microecology.</p><h3 data-test=\\\"abstract-sub-heading\\\">Results</h3><p>The results revealed rhizosphere bacterial community composition shifted notably with escalating parasite number. Bio-organic fertilizers application markedly upregulated the expression of <i>Mn-SOD</i>, <i>GST</i>, <i>XTH9</i>, <i>ACC01</i> and <i>NPR1</i> genes associated with plant resistance, and significantly increased the levels of the fruit ripening-related hormones ACC and tZTR. Application of bio-organic fertilizer remolded the rhizosphere microbial structure and significantly increased seed yield and crude protein content of sunflower. Upon applying bio-organic fertilizers, <i>O. cumana</i> roots exhibited decay-inducing fungal colonization, yet the isolated strain <i>Fusarium solani</i> L5 significantly reducing the number of parasites.</p><h3 data-test=\\\"abstract-sub-heading\\\">Conclusions</h3><p>Bio-organic fertilizers demonstrated efficacy in suppressing <i>O. cumana</i> parasitism while enhancing sunflower productivity and quality. The results enhance our understanding of bio-organic fertilizers for controlling parasitic plants, offering insights into overcoming agricultural challenges through rhizosphere microbial communities.</p>\",\"PeriodicalId\":20223,\"journal\":{\"name\":\"Plant and Soil\",\"volume\":\"131 1\",\"pages\":\"\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2025-05-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant and Soil\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s11104-025-07542-z\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant and Soil","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11104-025-07542-z","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
Bio-organic fertilizer mitigates Orobanche cumana parasitism by regulating sunflower transcriptomes and rhizosphere microbial community
Background and aims
Orobanche cumana Wallr. is a holoparasitic weed that poses an increasing threat to sunflower cultivation. However, the influence of bio-organic fertilizers on O. cumana, remains inadequately understood. This study investigates the relationship between sunflower microbial community and O. cumana parasitism, as well as the inhibitory mechanism of bio-organic fertilizers on O. cumana.
Methods
Field investigation was conducted to study the response mechanisms of host microbial communities to parasitic plants using amplicon techniques. Pot experiment, the transcriptome was employed to investigate the mechanism by which bio-organic fertilizer inhibits O. cumana parasitism. Field experiments were conducted to explore changes in sunflower yield, quality and rhizosphere microecology.
Results
The results revealed rhizosphere bacterial community composition shifted notably with escalating parasite number. Bio-organic fertilizers application markedly upregulated the expression of Mn-SOD, GST, XTH9, ACC01 and NPR1 genes associated with plant resistance, and significantly increased the levels of the fruit ripening-related hormones ACC and tZTR. Application of bio-organic fertilizer remolded the rhizosphere microbial structure and significantly increased seed yield and crude protein content of sunflower. Upon applying bio-organic fertilizers, O. cumana roots exhibited decay-inducing fungal colonization, yet the isolated strain Fusarium solani L5 significantly reducing the number of parasites.
Conclusions
Bio-organic fertilizers demonstrated efficacy in suppressing O. cumana parasitism while enhancing sunflower productivity and quality. The results enhance our understanding of bio-organic fertilizers for controlling parasitic plants, offering insights into overcoming agricultural challenges through rhizosphere microbial communities.
期刊介绍：
Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.
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