表达苏云金芽孢杆菌单蛋白和锥体蛋白的棉花对棉铃虫、斜纹夜蛾和果纹夜蛾的致死和亚致死效应

Sharna Holman, Paul Grundy, Helen Spafford, Michael Furlong
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引用次数: 0

摘要

鳞翅目害虫对苏云金芽孢杆菌(Bt)蛋白的敏感性不同。虽然Bollgard 3棉花(BG3)在澳大利亚能有效控制主要害虫棉铃虫Helicoverpa armigera (h bner),但其对其他害虫如斜纹夜蛾(Fabricius)和frugiperda (j.e. Smith)的有效性尚不清楚。本实验以非转基因棉花品种(CC)和3个转基因棉花品种(表达Cry1Ac的Bollgard (BG1)、表达Cry1Ac和Cry2Ab的Bollgard II (BG2)和表达Cry1Ac、Cry2Ab和Vip3A的Bollgard 3 (BG3))的叶片为食,对棉蚜、litura和frugiperda幼虫的存活和发育进行了研究。与CC和BG1相比,Bt锥体棉对各品种的生存发育均有较大的负面影响。幼虫取食BG2或BG3时,成虫比例明显低于BG1。斜纹夜蛾和果纹夜蛾在BG3上的羽化率明显低于BG2和BG1。本研究表明,与以前的Bt棉产品相比,BG3对更广泛的鳞翅目害虫具有更强的防治效果。尽管在实验室中有药效,但据报道斜纹夜蛾幼虫在BG3田间存活,这表明其他因素正在影响田间药效。随着BG3在澳大利亚北部热带地区的生产扩大,保持S. litura和S. frugiperda对BG3蛋白的敏感性至关重要。本研究表明,需要进一步研究次生害虫的田间生存和抗性管理策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Lethal and sublethal effects of cotton expressing single and pyramided proteins of Bacillus thuringiensis (Bt) on Helicoverpa armigera (Lepidoptera: Noctuidae), Spodoptera litura (Lepidoptera: Noctuidae), and Spodoptera frugiperda (Lepidoptera: Noctuidae).

The susceptibility to proteins from Bacillus thuringiensis (Bt) can vary among lepidopteran pest species. While Bollgard 3 cotton (BG3) effectively controls the primary pest Helicoverpa armigera (Hübner) in Australia, its effectiveness against other pests, such as Spodoptera litura (Fabricius) and Spodoptera frugiperda (J. E. Smith), is unknown. This laboratory study assessed the survival and development of H. armigera, S. litura, and S. frugiperda larvae when fed foliage from a non-transgenic cotton variety (CC) and 3 transgenic cotton varieties: Bollgard (BG1) expressing Cry1Ac, Bollgard II (BG2) expressing Cry1Ac and Cry2Ab, and Bollgard 3 (BG3) expressing Cry1Ac, Cry2Ab, and Vip3A. Pyramided Bt cotton had greater negative effects on survival and development of all species compared with CC or BG1. The proportion of H. armigera that eclosed as adults was very low when larvae fed on BG2 or BG3 compared with BG1. Eclosion rates of S. litura and S. frugiperda on BG3 were much lower compared with BG2 and BG1. This study demonstrates that BG3 has greater efficacy against a wider lepidopteran pest complex compared with previous Bt cotton products. Despite efficacy in the laboratory, S. litura larvae are reported to be surviving in BG3 fields, suggesting other factors are influencing field efficacy. As BG3 production expands across tropical northern Australia, preserving the susceptibility of S. litura and S. frugiperda to BG3 proteins is crucial. This study identifies the need for further research on field survival and resistance management strategies for secondary pest species.

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