埃塞俄比亚亚的斯亚贝巴市来自商业家禽养殖场和零售商店的生鸡蛋中沙门氏菌的流行率、风险因素和抗菌药物敏感性概况以及接触者

IF 2.1 4区农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of food protection Pub Date : 2025-05-16 DOI:10.1016/j.jfp.2025.100544

Paulos Adane , Tekalign Tadesse , Haile Alemayehu , Tadesse Eguale

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引用次数: 0

摘要

尽管亚的斯亚贝巴有广泛的家禽养殖场，但关于养殖场和零售商店的沙门氏菌禽蛋状况以及分离株的抗微生物敏感性的信息有限。本研究旨在调查埃塞俄比亚亚的斯亚贝巴与家禽接触的人的生鸡蛋和粪便样本中沙门氏菌的流行情况、相关危险因素和抗菌敏感性。横断面研究于2022年11月至2023年7月进行。采用多阶段抽样技术，从55个农场和44家零售商店与家禽接触的人身上收集了392个鸡蛋样本（每个样本2个鸡蛋）和48个粪便样本。采用标准细菌学方法分离和鉴定沙门氏菌，并通过PCR对推定分离株进行验证。采用Kirby-Bauer纸片扩散法测定分离菌株对12种抗菌药物的敏感性。沙门氏菌蛋的总感染率为7.1%(28/392)，蛋壳的感染率最高，为21.4%（21/196）。从养殖场采集的鸡蛋中分离出的沙门氏菌最多，为8.2%（16/196）。在与家禽接触的人的粪便样本中未检出沙门氏菌。没有清洗脏鸡蛋的农场，处理鸡蛋前洗手习惯不佳的农场，或者没有将破裂的鸡蛋分开的农场与沙门氏菌阳性显著相关

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Prevalence, Risk Factors and Antimicrobial Susceptibility Profile of Salmonella in Raw Chicken Eggs Sourced from Commercial Poultry Farms and Retail Shops and In-contact Humans in Addis Ababa, Ethiopia

Despite the wide availability of poultry farms in Addis Ababa, limited information is available on the status of Salmonella in poultry eggs from both farms and retail stores, as well as the antimicrobial susceptibility of isolates. This study aimed to investigate the prevalence, associated risk factors, and antimicrobial susceptibility of Salmonella in raw chicken eggs and stool samples from humans in contact with poultry in Addis Ababa, Ethiopia. A cross-sectional study was conducted from November 2022 to July 2023. A multistage sampling technique was used to collect 392 pooled egg samples (two eggs per sample) and 48 stool samples from humans in contact with poultry, across 55 farms and 44 retail shops. Standard bacteriological methods were employed to isolate and identify Salmonella, and presumptive isolates were confirmed by PCR. The susceptibility of the isolates to 12 antimicrobials was assessed using the Kirby-Bauer disk diffusion method. The overall prevalence of Salmonella in eggs was 7.1% (28/392), with the highest prevalence observed in egg shells 21.4% (21/196). Most Salmonella isolates were recovered from eggs sampled at farms 8.2% (16/196). No Salmonella was detected in stool samples from humans in contact with poultry. Farms that did not clean dirty eggs, those with poor handwashing practices before handling eggs, or that did not separate cracked eggs were significantly associated with Salmonella positivity (p < 0.05). Among the 28 Salmonella isolates, 53.6% (15/28) and 46.6% (13/28) were resistant to streptomycin and tetracycline, respectively, and multidrug resistance to 3 or more antimicrobials was recorded in 8 (28.6%) of Salmonella isolates. The presence of antimicrobial-resistant Salmonella in chicken eggs is indicative of a significant public health concern. Raising public awareness about antimicrobial-resistant Salmonella and implementing appropriate food safety measures are essential to reduce the risk of zoonotic salmonellosis.

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来源期刊

Journal of food protection 工程技术-生物工程与应用微生物

CiteScore

4.20

自引率

5.00%

发文量

296

审稿时长

2.5 months

期刊介绍： The Journal of Food Protection® (JFP) is an international, monthly scientific journal in the English language published by the International Association for Food Protection (IAFP). JFP publishes research and review articles on all aspects of food protection and safety. Major emphases of JFP are placed on studies dealing with: Tracking, detecting (including traditional, molecular, and real-time), inactivating, and controlling food-related hazards, including microorganisms (including antibiotic resistance), microbial (mycotoxins, seafood toxins) and non-microbial toxins (heavy metals, pesticides, veterinary drug residues, migrants from food packaging, and processing contaminants), allergens and pests (insects, rodents) in human food, pet food and animal feed throughout the food chain; Microbiological food quality and traditional/novel methods to assay microbiological food quality; Prevention of food-related hazards and food spoilage through food preservatives and thermal/non-thermal processes, including process validation; Food fermentations and food-related probiotics; Safe food handling practices during pre-harvest, harvest, post-harvest, distribution and consumption, including food safety education for retailers, foodservice, and consumers; Risk assessments for food-related hazards; Economic impact of food-related hazards, foodborne illness, food loss, food spoilage, and adulterated foods; Food fraud, food authentication, food defense, and foodborne disease outbreak investigations.