从综合小农户生鲜农场分离的ESKAPE-E多重耐药病原菌的发生及基因型特征

IF 2.1 4区农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of food protection Pub Date : 2025-05-16 DOI:10.1016/j.jfp.2025.100543

Sheldon Viviers , Loandi Richter-Mouton , Jonathan Featherston , Lise Korsten

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引用次数: 0

摘要

抗微生物药物耐药性（AMR）是全球十大公共卫生威胁之一，产生广谱β-内酰胺酶（ESBL）的肠杆菌和铜绿假单胞菌成为多重耐药（MDR）重点病原体。本研究旨在评估从南非小农户生鲜农场的水-土壤-植物关系中分离出的产ESBL/ ampc的肠杆菌和铜绿假单胞菌的存在和特征（表型和基因型AMR谱）。从6个小农农场收集了灌溉水（n=44）、土壤（n=85）和新鲜农产品（n=95）样本。使用选择性富集、显色培养基分离潜在的MDR细菌，并使用MALDI-TOF ms进行鉴定，通过双盘扩散评估对13种不同抗生素的耐药性，同时使用全基因组测序（Illumina MiSeq）对20个选定的菌株进行基因典型表征。在17%（38/224）的样品中检测到产生ESBL/ ampc的分离株，主要来自新鲜农产品（54%），其次是土壤（31%）和水（15%）。从3种基质中鉴定出的优势肠杆菌种包括肠杆菌（26%）、克雷伯氏菌（26%）、沙雷氏菌（17%）和大肠杆菌（9%）。表型上，47%和43%的分离株分别被归类为ESBL和ampc生产者，对β -内酰胺类、氨基糖苷类和甘环素类具有抗性。值得注意的是，WGS分析表明，在来自环境的分离株中存在临床显著的AMR和毒力基因，特别是那些与移动遗传元件相关的基因。该研究的结果增加了对小农农业领域AMR流行情况的有限的国家和国际知识，需要引入适当的干预策略和未来非正式供应链的风险评估。

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Occurrence and Genotypic Characterization of Selected Multidrug-resistant ESKAPE-E Pathogens Isolated from Integrated Smallholder Fresh Produce Farms

Antimicrobial resistance (AMR) ranks among the top ten global public health threats, with extended-spectrum β-lactamase (ESBL)-producing Enterobacterales and Pseudomonas aeruginosa emerging as multidrug-resistant (MDR) priority pathogens. This study aimed to evaluate the presence and characteristics (phenotypic and genotypic AMR profiles) of ESBL/AmpC-producing Enterobacterales and P. aeruginosa isolates obtained from the water-soil-plant nexus on smallholder fresh produce farms in South Africa. Samples of irrigation water (n = 44), soil (n = 85), and fresh produce (n = 95) were collected from six smallholder farms. Potential MDR bacteria were isolated using selective enrichment, chromogenic media, and identified with MALDI-TOF MS. Antimicrobial resistance was assessed via double-disk diffusion against 13 different antibiotic classes, while 20 selected isolates were genotypically characterized using whole-genome sequencing (WGS) (Illumina MiSeq). ESBL/AmpC-producing isolates were detected in 17% of samples (38/224), primarily from fresh produce (54%), followed by soil (31%) and water (15%). The dominant Enterobacterales species identified from all three matrices included Enterobacter spp. (26%), Klebsiella spp. (26%), Serratia spp. (17%), and Escherichia coli (9%). Phenotypically, 47% and 43% of the isolates were classified as ESBL and AmpC-producers, respectively, with resistance to beta-lactams, aminoglycosides, and glycylcyclines. Notably, WGS analysis indicated the presence of clinically significant AMR and virulence genes among isolates from the environment, particularly those associated with mobile genetic elements. The results from the study add to the limited national and international knowledge of AMR prevalence in the smallholder agricultural landscape, needed for the introduction of appropriate intervention strategies and future risk assessment of the informal supply chain.

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来源期刊

Journal of food protection 工程技术-生物工程与应用微生物

CiteScore

4.20

自引率

5.00%

发文量

296

审稿时长

2.5 months

期刊介绍： The Journal of Food Protection® (JFP) is an international, monthly scientific journal in the English language published by the International Association for Food Protection (IAFP). JFP publishes research and review articles on all aspects of food protection and safety. Major emphases of JFP are placed on studies dealing with: Tracking, detecting (including traditional, molecular, and real-time), inactivating, and controlling food-related hazards, including microorganisms (including antibiotic resistance), microbial (mycotoxins, seafood toxins) and non-microbial toxins (heavy metals, pesticides, veterinary drug residues, migrants from food packaging, and processing contaminants), allergens and pests (insects, rodents) in human food, pet food and animal feed throughout the food chain; Microbiological food quality and traditional/novel methods to assay microbiological food quality; Prevention of food-related hazards and food spoilage through food preservatives and thermal/non-thermal processes, including process validation; Food fermentations and food-related probiotics; Safe food handling practices during pre-harvest, harvest, post-harvest, distribution and consumption, including food safety education for retailers, foodservice, and consumers; Risk assessments for food-related hazards; Economic impact of food-related hazards, foodborne illness, food loss, food spoilage, and adulterated foods; Food fraud, food authentication, food defense, and foodborne disease outbreak investigations.