Ellia Kartini Mujar, Saleema Matusin, Nur Syahirah Yahya, Muhammad Safwan Ahamad Bustamam, Annie Christianus, Khozirah Shaari, Nur Kartinee Kassim, Faridah Abas, Jen Kit Tan, Intan Safinar Ismail
{"title":"利用LCMS/MS-based黏液和血清代谢组学研究杂交石斑鱼(Epinephelus fuscoguttatus X Epinephelus lanceolatus)对温度波动胁迫的生理响应","authors":"Ellia Kartini Mujar, Saleema Matusin, Nur Syahirah Yahya, Muhammad Safwan Ahamad Bustamam, Annie Christianus, Khozirah Shaari, Nur Kartinee Kassim, Faridah Abas, Jen Kit Tan, Intan Safinar Ismail","doi":"10.1007/s10695-025-01510-1","DOIUrl":null,"url":null,"abstract":"<p><p>Aquaculture, which involves the cultivation of aquatic animals and plants, is recognised to be an effective and vital resource-efficient food production system for supplying affordable protein to humans. Hybrid grouper (Epinephelus fuscoguttatus x Epinephelus lanceolatus) is a marine carnivorous teleost that has a high economic importance in the marine aquaculture industry particularly in countries in the Asian region. However, the vulnerability of juvenile hybrid grouper to temperature fluctuations stemming from the acceleration of climate change is regarded as one of significant threats to their market supply and a major concern for the aquaculture industry. In the present study, LCMS/MS-based mucus and sera metabolomics were used to investigate the physiological responses of juvenile hybrid grouper via their behavioural response towards temperature fluctuation stress that could be translated as indicators for either resilience or susceptibility to the stressors. Susceptible fish exhibited pronounced behavioural responses, including cessation of feeding and erratic swimming. In contrast, resilient fish displayed relatively stable behaviour, comparable to control fish, with reduced feeding as a primary deviation. A total of 36 mucus metabolites (VIP > 1) were profiled in OPLSDA in juvenile hybrid grouper subjected to temperature fluctuation stress. Among these, 20 and 16 metabolites were significantly altered (Student's t-test, p < 0.05) in susceptible (MSG) and resilient (MRG) fish in comparison to the control (CG), respectively. Although OPLSDA did not reveal a clear separation between the two groups, 4 metabolites namely glycerophosphocholine, N-acetylgalactosamine, N-acetylglucosamine, and pantothenic acid differed significantly between them. In sera, 54 metabolites (VIP > 1) were profiled, with 38 and 26 significantly altered in the susceptible (SSG) and resilient (SRG) groups, respectively. Comparative analysis between significant and resilient groups resulted in the nomination of hypoxanthine, guanosine, guanine, methionine, DL-malic acid, and glucose as potential biomarkers for susceptibility in juvenile hybrid grouper. Pathway analysis of these metabolite markers revealed that temperature stress mainly affected purine metabolism, TCA cycle, cysteine and methionine metabolism, and pyruvate metabolism. Overall, the findings of this study provide insights into the physiological regulation of juvenile hybrid grouper that are susceptible to temperature changes through their behavioural response, as well as a framework for future research.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"99"},"PeriodicalIF":2.5000,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Physiological responses of hybrid grouper (Epinephelus fuscoguttatus X Epinephelus lanceolatus) to temperature fluctuation stress revealed by LCMS/MS-based mucus and sera metabolomics.\",\"authors\":\"Ellia Kartini Mujar, Saleema Matusin, Nur Syahirah Yahya, Muhammad Safwan Ahamad Bustamam, Annie Christianus, Khozirah Shaari, Nur Kartinee Kassim, Faridah Abas, Jen Kit Tan, Intan Safinar Ismail\",\"doi\":\"10.1007/s10695-025-01510-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Aquaculture, which involves the cultivation of aquatic animals and plants, is recognised to be an effective and vital resource-efficient food production system for supplying affordable protein to humans. 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In contrast, resilient fish displayed relatively stable behaviour, comparable to control fish, with reduced feeding as a primary deviation. A total of 36 mucus metabolites (VIP > 1) were profiled in OPLSDA in juvenile hybrid grouper subjected to temperature fluctuation stress. Among these, 20 and 16 metabolites were significantly altered (Student's t-test, p < 0.05) in susceptible (MSG) and resilient (MRG) fish in comparison to the control (CG), respectively. Although OPLSDA did not reveal a clear separation between the two groups, 4 metabolites namely glycerophosphocholine, N-acetylgalactosamine, N-acetylglucosamine, and pantothenic acid differed significantly between them. In sera, 54 metabolites (VIP > 1) were profiled, with 38 and 26 significantly altered in the susceptible (SSG) and resilient (SRG) groups, respectively. Comparative analysis between significant and resilient groups resulted in the nomination of hypoxanthine, guanosine, guanine, methionine, DL-malic acid, and glucose as potential biomarkers for susceptibility in juvenile hybrid grouper. Pathway analysis of these metabolite markers revealed that temperature stress mainly affected purine metabolism, TCA cycle, cysteine and methionine metabolism, and pyruvate metabolism. 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引用次数: 0
摘要
水产养殖涉及水生动植物的养殖,被认为是一种有效和重要的资源节约型粮食生产系统,可为人类提供负担得起的蛋白质。杂交石斑鱼(Epinephelus fuscoguttatus x Epinephelus lanceolatus)是一种海洋肉食性硬骨鱼,在海洋水产养殖业中具有很高的经济价值,特别是在亚洲地区的国家。然而,杂交石斑鱼幼鱼对气候变化加速引起的温度波动的脆弱性被认为是对其市场供应的重大威胁之一,也是水产养殖业关注的主要问题。在本研究中,利用LCMS/MS-based黏液和血清代谢组学研究杂交石斑鱼幼鱼对温度波动胁迫的生理反应,通过对温度波动胁迫的行为反应,可以转化为对应激源的恢复力或易感性的指标。易感鱼表现出明显的行为反应,包括停止进食和不稳定的游泳。相比之下,弹性鱼表现出相对稳定的行为,与对照鱼相当,摄食减少是主要偏差。在温度波动胁迫下,杂交石斑鱼幼鱼的OPLSDA中共检测到36种黏液代谢物(VIP >1)。其中,20和16种代谢物显著改变(学生t检验,p = 1),易感组(SSG)和弹性组(SRG)分别有38和26种代谢物显著改变。通过对显著组和弹性组的比较分析,提出了次黄嘌呤、鸟苷、鸟嘌呤、蛋氨酸、dl -苹果酸和葡萄糖作为杂交石斑鱼幼鱼易感性的潜在生物标志物。这些代谢标志物的通路分析表明,温度胁迫主要影响了嘌呤代谢、TCA循环、半胱氨酸和蛋氨酸代谢以及丙酮酸代谢。总的来说,本研究的发现提供了对杂交石斑鱼幼鱼易受温度变化影响的生理调节的见解,并为未来的研究提供了框架。
Physiological responses of hybrid grouper (Epinephelus fuscoguttatus X Epinephelus lanceolatus) to temperature fluctuation stress revealed by LCMS/MS-based mucus and sera metabolomics.
Aquaculture, which involves the cultivation of aquatic animals and plants, is recognised to be an effective and vital resource-efficient food production system for supplying affordable protein to humans. Hybrid grouper (Epinephelus fuscoguttatus x Epinephelus lanceolatus) is a marine carnivorous teleost that has a high economic importance in the marine aquaculture industry particularly in countries in the Asian region. However, the vulnerability of juvenile hybrid grouper to temperature fluctuations stemming from the acceleration of climate change is regarded as one of significant threats to their market supply and a major concern for the aquaculture industry. In the present study, LCMS/MS-based mucus and sera metabolomics were used to investigate the physiological responses of juvenile hybrid grouper via their behavioural response towards temperature fluctuation stress that could be translated as indicators for either resilience or susceptibility to the stressors. Susceptible fish exhibited pronounced behavioural responses, including cessation of feeding and erratic swimming. In contrast, resilient fish displayed relatively stable behaviour, comparable to control fish, with reduced feeding as a primary deviation. A total of 36 mucus metabolites (VIP > 1) were profiled in OPLSDA in juvenile hybrid grouper subjected to temperature fluctuation stress. Among these, 20 and 16 metabolites were significantly altered (Student's t-test, p < 0.05) in susceptible (MSG) and resilient (MRG) fish in comparison to the control (CG), respectively. Although OPLSDA did not reveal a clear separation between the two groups, 4 metabolites namely glycerophosphocholine, N-acetylgalactosamine, N-acetylglucosamine, and pantothenic acid differed significantly between them. In sera, 54 metabolites (VIP > 1) were profiled, with 38 and 26 significantly altered in the susceptible (SSG) and resilient (SRG) groups, respectively. Comparative analysis between significant and resilient groups resulted in the nomination of hypoxanthine, guanosine, guanine, methionine, DL-malic acid, and glucose as potential biomarkers for susceptibility in juvenile hybrid grouper. Pathway analysis of these metabolite markers revealed that temperature stress mainly affected purine metabolism, TCA cycle, cysteine and methionine metabolism, and pyruvate metabolism. Overall, the findings of this study provide insights into the physiological regulation of juvenile hybrid grouper that are susceptible to temperature changes through their behavioural response, as well as a framework for future research.
期刊介绍:
Fish Physiology and Biochemistry is an international journal publishing original research papers in all aspects of the physiology and biochemistry of fishes. Coverage includes experimental work in such topics as biochemistry of organisms, organs, tissues and cells; structure of organs, tissues, cells and organelles related to their function; nutritional, osmotic, ionic, respiratory and excretory homeostasis; nerve and muscle physiology; endocrinology; reproductive physiology; energetics; biochemical and physiological effects of toxicants; molecular biology and biotechnology and more.