Nayara Maria Gil Mazzante, Fernanda Zuliani, Rogerio Antonio de Oliveira, Júlia Soares Bodaneze, Giovanna Farina Panebianco, Natália Freitas de Souza, Fernando Carmona Dinau, Paola Alejandra Montenegro Cuellar, Nadia Yumi Yamamo Dos Santos, Ana Beatriz de Souza da Silva, Fernanda de Freitas Alves Vieira, Natália Camargo Faraldo, Gabriela Abreu Botelho, Fernanda Barthelson Carvalho de Moura, Noeme Sousa Rocha
{"title":"不同死亡诱导程度wistar大鼠脑死亡诊断。","authors":"Nayara Maria Gil Mazzante, Fernanda Zuliani, Rogerio Antonio de Oliveira, Júlia Soares Bodaneze, Giovanna Farina Panebianco, Natália Freitas de Souza, Fernando Carmona Dinau, Paola Alejandra Montenegro Cuellar, Nadia Yumi Yamamo Dos Santos, Ana Beatriz de Souza da Silva, Fernanda de Freitas Alves Vieira, Natália Camargo Faraldo, Gabriela Abreu Botelho, Fernanda Barthelson Carvalho de Moura, Noeme Sousa Rocha","doi":"10.12688/f1000research.157233.2","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate hematologic, biochemical, and gasometric parameters in Wistar rats to better understand brain death parameters and reduce early misdiagnoses.</p><p><strong>Methods: </strong>Fifteen adult male Wistar rats (Rattus norvergicus; HanUnib: WH) were randomly distributed into three groups of five animals: the control group (G0) with evaluation performed before brain death, and two groups (G1 and G2) with brain death induced at different times: immediately after induction (G1) and one hour after induction (G2). Venous and arterial blood samples were taken to perform complete blood count, biochemical, and blood gas assays. Samples were taken at specific times based on the group each rat belonged to.</p><p><strong>Results: </strong>Statistically significant mean values were observed (P<0,05) for segmented cells (G1>G2 and G0>G2), monocytes (G2>G1 and G0>G1), creatinine (G2>G0), aspartate aminotransferase (G1>G0), potassium (G2>G0), and bicarbonate (G0>G1).</p><p><strong>Discussion: </strong>Furthermore, brain death showed a unique response in each organism, complicating its precise determination even more.</p>","PeriodicalId":12260,"journal":{"name":"F1000Research","volume":"13 ","pages":"1490"},"PeriodicalIF":0.0000,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082075/pdf/","citationCount":"0","resultStr":"{\"title\":\"Diagnosis of brain death in wistar rats at different levels of death induction.\",\"authors\":\"Nayara Maria Gil Mazzante, Fernanda Zuliani, Rogerio Antonio de Oliveira, Júlia Soares Bodaneze, Giovanna Farina Panebianco, Natália Freitas de Souza, Fernando Carmona Dinau, Paola Alejandra Montenegro Cuellar, Nadia Yumi Yamamo Dos Santos, Ana Beatriz de Souza da Silva, Fernanda de Freitas Alves Vieira, Natália Camargo Faraldo, Gabriela Abreu Botelho, Fernanda Barthelson Carvalho de Moura, Noeme Sousa Rocha\",\"doi\":\"10.12688/f1000research.157233.2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>This study aimed to evaluate hematologic, biochemical, and gasometric parameters in Wistar rats to better understand brain death parameters and reduce early misdiagnoses.</p><p><strong>Methods: </strong>Fifteen adult male Wistar rats (Rattus norvergicus; HanUnib: WH) were randomly distributed into three groups of five animals: the control group (G0) with evaluation performed before brain death, and two groups (G1 and G2) with brain death induced at different times: immediately after induction (G1) and one hour after induction (G2). Venous and arterial blood samples were taken to perform complete blood count, biochemical, and blood gas assays. Samples were taken at specific times based on the group each rat belonged to.</p><p><strong>Results: </strong>Statistically significant mean values were observed (P<0,05) for segmented cells (G1>G2 and G0>G2), monocytes (G2>G1 and G0>G1), creatinine (G2>G0), aspartate aminotransferase (G1>G0), potassium (G2>G0), and bicarbonate (G0>G1).</p><p><strong>Discussion: </strong>Furthermore, brain death showed a unique response in each organism, complicating its precise determination even more.</p>\",\"PeriodicalId\":12260,\"journal\":{\"name\":\"F1000Research\",\"volume\":\"13 \",\"pages\":\"1490\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-05-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082075/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"F1000Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.12688/f1000research.157233.2\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"Pharmacology, Toxicology and Pharmaceutics\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"F1000Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12688/f1000research.157233.2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
Diagnosis of brain death in wistar rats at different levels of death induction.
Objective: This study aimed to evaluate hematologic, biochemical, and gasometric parameters in Wistar rats to better understand brain death parameters and reduce early misdiagnoses.
Methods: Fifteen adult male Wistar rats (Rattus norvergicus; HanUnib: WH) were randomly distributed into three groups of five animals: the control group (G0) with evaluation performed before brain death, and two groups (G1 and G2) with brain death induced at different times: immediately after induction (G1) and one hour after induction (G2). Venous and arterial blood samples were taken to perform complete blood count, biochemical, and blood gas assays. Samples were taken at specific times based on the group each rat belonged to.
Results: Statistically significant mean values were observed (P<0,05) for segmented cells (G1>G2 and G0>G2), monocytes (G2>G1 and G0>G1), creatinine (G2>G0), aspartate aminotransferase (G1>G0), potassium (G2>G0), and bicarbonate (G0>G1).
Discussion: Furthermore, brain death showed a unique response in each organism, complicating its precise determination even more.
F1000ResearchPharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
5.00
自引率
0.00%
发文量
1646
审稿时长
1 weeks
期刊介绍：
F1000Research publishes articles and other research outputs reporting basic scientific, scholarly, translational and clinical research across the physical and life sciences, engineering, medicine, social sciences and humanities. F1000Research is a scholarly publication platform set up for the scientific, scholarly and medical research community; each article has at least one author who is a qualified researcher, scholar or clinician actively working in their speciality and who has made a key contribution to the article. Articles must be original (not duplications). All research is suitable irrespective of the perceived level of interest or novelty; we welcome confirmatory and negative results, as well as null studies. F1000Research publishes different type of research, including clinical trials, systematic reviews, software tools, method articles, and many others. Reviews and Opinion articles providing a balanced and comprehensive overview of the latest discoveries in a particular field, or presenting a personal perspective on recent developments, are also welcome. See the full list of article types we accept for more information.
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