用磷光终身成像定量视网膜氧合和代谢。

IF 3 2区医学 Q1 OPHTHALMOLOGY

Experimental eye research Pub Date : 2025-05-15 DOI:10.1016/j.exer.2025.110422

Mahnaz Shahidi

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引用次数: 0

摘要

视网膜是一个高度代谢活跃的组织，需要足够的氧气和其他代谢物来产生细胞生存和视觉功能所需的能量。视网膜缺氧与几种常见的视网膜疾病有关，并与视觉威胁病理的发展有关。由于缺氧水平决定了细胞存活或死亡的激活过程，因此了解视网膜氧化是必不可少的。本文综述了利用磷光终身成像技术对啮齿动物视网膜血管和组织氧张力的深度分辨定量测量。此外，从氧张力测量中定量得出视网膜氧代谢生物标志物，并显示在受到挑战的生理和病理条件下发生改变。应用磷光终身成像技术可以提高对视网膜缺血病理生理学的认识，识别生理生物标志物，以监测人类视网膜疾病动物模型的进展和评估治疗干预措施。

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Quantifying retinal oxygenation and metabolism by phosphorescence lifetime imaging

The retina is a highly metabolically active tissue, requiring adequate availability of oxygen and other metabolites to generate energy for cellular survival and visual function. Retinal hypoxia has been implicated in several common retinal diseases and associated with the development of vision-threatening pathologies. Since the level of hypoxia determines processes that are activated for either cell survival or death, knowledge of retinal oxygenation is essential. This article reviews depth-resolved quantitative measurements of retinal vascular and tissue oxygen tension in rodents using the technique of phosphorescence lifetime imaging. Furthermore, retinal oxygen metabolic biomarkers were quantitatively derived from oxygen tension measurements and shown to be altered under challenged physiological and pathological conditions. Application of phosphorescence lifetime imaging can be useful for advancing knowledge of retinal ischemia pathophysiology and identifying physiological biomarkers to monitor progression and evaluate therapeutic interventions in animal models of human retinal diseases.

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来源期刊

Experimental eye research 医学-眼科学

CiteScore

6.80

自引率

5.90%

发文量

323

审稿时长

66 days

期刊介绍： The primary goal of Experimental Eye Research is to publish original research papers on all aspects of experimental biology of the eye and ocular tissues that seek to define the mechanisms of normal function and/or disease. Studies of ocular tissues that encompass the disciplines of cell biology, developmental biology, genetics, molecular biology, physiology, biochemistry, biophysics, immunology or microbiology are most welcomed. Manuscripts that are purely clinical or in a surgical area of ophthalmology are not appropriate for submission to Experimental Eye Research and if received will be returned without review.