Jiantao Zeng, Dan Yang, Ke Nie, Dingbin Liu, Huahong Ye, Tao Dai
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Screening of β-Thalassemia Carriers based on Matrix Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry.
Background: The traditional analytical methods for β-thalassemia carriers are time-consuming and costly. This study takes advantage of matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to directly analyze human whole blood samples and established a preliminary method for screening β-thalassemia carriers.
Methods: A sample of 3 µL whole blood was diluted 200-fold with distilled water, mixed with sinapic acid, and subjected to MALDI-TOF MS analysis. The efficacy of the method for the screening of β-thalassemia carriers was assessed using the β-thalassemia gene results as the standard.
Results: β-thalassemia gene results showed 109 β-thalassemia carriers and 110 normal individuals out of 219 samples. We developed a δ-globin peak slope index that can simply and effectively distinguish β-thalassemia carriers from healthy individuals, the best cutoff value for the slope index was -0.819.
Conclusions: MALDI-TOF MS has the potential to be a valuable tool for β-thalassemia carriers screening.
期刊介绍：
Clinical Laboratory is an international fully peer-reviewed journal covering all aspects of laboratory medicine and transfusion medicine. In addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies. The journal publishes original articles, review articles, posters, short reports, case studies and letters to the editor dealing with 1) the scientific background, implementation and diagnostic significance of laboratory methods employed in hospitals, blood banks and physicians'' offices and with 2) scientific, administrative and clinical aspects of transfusion medicine and 3) in addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies.
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