{"title":"用于肺组织中石棉分析的冷冻干燥/等离子asher方法的验证。","authors":"Anna Benedetta Somigliana","doi":"10.3389/fchem.2025.1581910","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>The analysis of asbestos fibers in lung tissue is complex due to both the biological matrix and the analyte. Lung tissue preparation techniques for asbestos burden analysis require the removal of organic matter to make the inorganic mineral components visible. The method's validation is challenging due to the analyte's inherent variability. This study outlines a procedure for validating an asbestos fiber analysis method in lung tissue.</p><p><strong>Methods: </strong>At the Electron Microscopy Laboratory of ARPA Lombardia, a method using a plasma asher for freeze-dried lung tissue digestion was developed. The analysis is carried out with a scanning electron microscope equipped with an energy-dispersive x-ray spectrometer. Problems associated with interferences, instrument calibration and resolution, analytical sensitivity, recovery were described in detail. The trueness and precision of the analytical method were evaluated using certified reference materials in accordance with ISO 33403:2024 standard.</p><p><strong>Results and conclusion: </strong>The developed method underwent a rigorous validation process to ensure metrological traceability of the results. Using validated analytical methods with consistent counting rules ensures comparability of data across laboratories while guaranteeing traceable results.</p>","PeriodicalId":12421,"journal":{"name":"Frontiers in Chemistry","volume":"13 ","pages":"1581910"},"PeriodicalIF":3.8000,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081419/pdf/","citationCount":"0","resultStr":"{\"title\":\"Validation of freeze-drier/plasma asher method for analysis of asbestos in the lung tissue.\",\"authors\":\"Anna Benedetta Somigliana\",\"doi\":\"10.3389/fchem.2025.1581910\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>The analysis of asbestos fibers in lung tissue is complex due to both the biological matrix and the analyte. Lung tissue preparation techniques for asbestos burden analysis require the removal of organic matter to make the inorganic mineral components visible. The method's validation is challenging due to the analyte's inherent variability. This study outlines a procedure for validating an asbestos fiber analysis method in lung tissue.</p><p><strong>Methods: </strong>At the Electron Microscopy Laboratory of ARPA Lombardia, a method using a plasma asher for freeze-dried lung tissue digestion was developed. The analysis is carried out with a scanning electron microscope equipped with an energy-dispersive x-ray spectrometer. Problems associated with interferences, instrument calibration and resolution, analytical sensitivity, recovery were described in detail. The trueness and precision of the analytical method were evaluated using certified reference materials in accordance with ISO 33403:2024 standard.</p><p><strong>Results and conclusion: </strong>The developed method underwent a rigorous validation process to ensure metrological traceability of the results. Using validated analytical methods with consistent counting rules ensures comparability of data across laboratories while guaranteeing traceable results.</p>\",\"PeriodicalId\":12421,\"journal\":{\"name\":\"Frontiers in Chemistry\",\"volume\":\"13 \",\"pages\":\"1581910\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2025-05-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081419/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.3389/fchem.2025.1581910\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.3389/fchem.2025.1581910","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Validation of freeze-drier/plasma asher method for analysis of asbestos in the lung tissue.
Introduction: The analysis of asbestos fibers in lung tissue is complex due to both the biological matrix and the analyte. Lung tissue preparation techniques for asbestos burden analysis require the removal of organic matter to make the inorganic mineral components visible. The method's validation is challenging due to the analyte's inherent variability. This study outlines a procedure for validating an asbestos fiber analysis method in lung tissue.
Methods: At the Electron Microscopy Laboratory of ARPA Lombardia, a method using a plasma asher for freeze-dried lung tissue digestion was developed. The analysis is carried out with a scanning electron microscope equipped with an energy-dispersive x-ray spectrometer. Problems associated with interferences, instrument calibration and resolution, analytical sensitivity, recovery were described in detail. The trueness and precision of the analytical method were evaluated using certified reference materials in accordance with ISO 33403:2024 standard.
Results and conclusion: The developed method underwent a rigorous validation process to ensure metrological traceability of the results. Using validated analytical methods with consistent counting rules ensures comparability of data across laboratories while guaranteeing traceable results.
期刊介绍：
Frontiers in Chemistry is a high visiblity and quality journal, publishing rigorously peer-reviewed research across the chemical sciences. Field Chief Editor Steve Suib at the University of Connecticut is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to academics, industry leaders and the public worldwide.
Chemistry is a branch of science that is linked to all other main fields of research. The omnipresence of Chemistry is apparent in our everyday lives from the electronic devices that we all use to communicate, to foods we eat, to our health and well-being, to the different forms of energy that we use. While there are many subtopics and specialties of Chemistry, the fundamental link in all these areas is how atoms, ions, and molecules come together and come apart in what some have come to call the “dance of life”.
All specialty sections of Frontiers in Chemistry are open-access with the goal of publishing outstanding research publications, review articles, commentaries, and ideas about various aspects of Chemistry. The past forms of publication often have specific subdisciplines, most commonly of analytical, inorganic, organic and physical chemistries, but these days those lines and boxes are quite blurry and the silos of those disciplines appear to be eroding. Chemistry is important to both fundamental and applied areas of research and manufacturing, and indeed the outlines of academic versus industrial research are also often artificial. Collaborative research across all specialty areas of Chemistry is highly encouraged and supported as we move forward. These are exciting times and the field of Chemistry is an important and significant contributor to our collective knowledge.
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