黏胶红霉菌AUMC13567在优化条件下对孔雀石绿染料毒性的生物修复

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Maysa M Ali, Somaya Nassar, Nivien Allam Nafady, Eman Mostafa Mohamed
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引用次数: 0

摘要

人类城市化和工业快速发展的影响不断升级,导致全球对合成纺织染料的需求不断增长。孔雀石绿(MG)染料是一种危险的彩色化合物,主要用作纺织染料,对所应用的基材具有很强的亲和力。稀释后,它可以作为养鱼场真菌和细菌疾病的抗菌剂。然而,纺织印染废料的处理构成了重大的环境挑战,污染土壤、地表水,并对人类健康和水生生物产生不利影响。即使在营养缺乏或外界胁迫的条件下,粘毛霉也表现出强劲的生长和抵抗能力。本研究主要对酵母菌粘胶红酵母AUMC13567降解孔雀石绿染料的能力进行优化。结果表明,在含3%葡萄糖、0.5%蛋白胨、0.3%麦芽提取物和0.3%酵母提取物的蒸馏水培养基上,在37˚C、150 rpm、有氧条件下培养12 h,染色浓度为50 mg/L时,mucilaginosa脱色率达到100%。气相色谱-质谱(GC/MS)分析证实了酵母降解有毒MG染料复合物为简单无害代谢物的功效。傅里叶变换红外(FTIR)分析表明,MG染料降解后形成新的化合物。建议进一步的研究,以确定和表征特定的酶或代谢途径涉及的毛胶霉AUMC13567降解MG染料。对三种人类细胞系(包括结直肠癌、头颈癌和健康皮肤成纤维细胞)进行的细胞毒性试验表明,脱色MG溶液产生的产品安全,对测试的人类细胞系没有细胞毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bioremediation of malachite green dye toxicity under optimized conditions by Rhodotorula mucilaginosa AUMC13567.

The escalating impacts of human urbanization and rapid industrial development have resulted in a growing global demand for synthetic textile dyes. Malachite green (MG) dye is a hazardous chromatic compound predominantly utilized as a textile dye, exhibiting a strong affinity for the substrate to which it is applied. When diluted, it serves as antimicrobial agent for fungal and bacterial diseases in fish farms. However, the disposal of textile dyeing wastes pose significant environmental challenges, contaminating soil, surface water, and adversely affecting human health and aquatic life. R. mucilaginosa exhibits robust growth and resistance capabilities even under conditions of nutrient deficiency or external stress. This study focuses on optimizing the malachite green dye degradation ability of the yeast strain Rhodotorula mucilaginosa AUMC13567. The results reveal that the optimal conditions for maximum decolorization which was 100% at 50 mg/L dye concentration involve cultivating R. mucilaginosa on medium comprising distilled water with 3% glucose, 0.5% peptone, 0.3% malt extract, and 0.3% yeast extract under aerobic conditions at 37˚C for 12 h at 150 rpm. Gas Chromatography-Mass Spectrometry (GC/MS) analysis confirms the yeast's efficacy in degrading toxic MG dye complex compounds into simple harmless metabolites. Fourier Transform Infrared (FTIR) analyses show formation of novel compounds post the degradation of MG dye. Further research is advised to identify and characterize the specific enzymes or metabolic pathways implicated in MG dye degradation by R. mucilaginosa AUMC13567. Cytotoxicity tests carried out on three human cell lines, including colorectal cancer, head and neck cancer, and healthy skin fibroblast, demonstrating that decolorized MG solution produces safe products devoid of cytotoxicity on tested human cell lines.

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来源期刊
BMC Biotechnology
BMC Biotechnology 工程技术-生物工程与应用微生物
CiteScore
6.60
自引率
0.00%
发文量
34
审稿时长
2 months
期刊介绍: BMC Biotechnology is an open access, peer-reviewed journal that considers articles on the manipulation of biological macromolecules or organisms for use in experimental procedures, cellular and tissue engineering or in the pharmaceutical, agricultural biotechnology and allied industries.
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