原生黏液中二硫键还原诱导的宏观异质性。

IF 4.7 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2025-06-16 Epub Date: 2025-05-19 DOI:10.1021/acsabm.4c01758
Giorgia Franzino, Fabiana Tescione, Domenico Larobina
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引用次数: 0

摘要

二硫键还原剂长期以来被用作黏液高分泌物的治疗药物(黏液解药)。已知二硫化物桥的断裂会导致交联程度的降低,使粘液更具流动性。除了粘弹性的下降,二硫化物的断裂也会在微观和中尺度上影响黏液的结构。尽管有这些知识,但在宏观尺度上对粘液的重组知之甚少。本文探讨还原剂三(2-羧基乙基)磷酸盐酸盐(TCEP)对天然猪胃粘液结构的影响。将黏液暴露于浓度不断增加的TCEP后,我们测量了黏液的宏观流变特性及其微观动力学。结果表明,TCEP的宏观异质性增加,我们将其归因于黏液相分离的趋势。此外,我们通过测量悬浮大分子的大小来检查不同还原黏液的可溶性组分。结果表明,随着TCEP浓度的增加,晶粒尺寸基本保持不变。我们的贡献可能是重要的,以描述二硫化还原剂对粘液结构的影响,从而对粘液纤毛和咳嗽清除。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Macroheterogeneities Induced by Disulfide Bond Reduction in Native Mucus.

Disulfide bond reducing agents have long been used as therapeutic drugs (mucolytics) for mucus hypersecretions. Breakage of disulfide bridges is known to cause a reduction in the degree of cross-linking, making the mucus more fluid. In addition to the drop in viscoelastic properties, the disulfide breakage is also known to affect the structure of the mucus on both micro- and mesoscale. Despite this knowledge, little is known about the reorganization of the mucus at the macroscopic scale. This contribution explores the effect of the reducing agent, tris(2-carboxyethyl)phosphine hydrochloride (TCEP), on the structure of native porcine gastric mucus. After exposing the mucus to increasing concentrations of TCEP, we measure the macroscopic rheological properties along with its microscopic dynamics. The results obtained show an increase in macroscopic heterogeneity with TCEP, which we attribute to the mucus tendency to phase separate. Furthermore, we examine the soluble fractions of the different reduced mucuses by measuring the size of the suspendable macromolecules. The results indicate that the size remains approximately constant with an increasing TCEP concentration. Our contribution may be important to describe the effects of a disulfide reducing agent on the mucus structure and, consequently, on mucociliary and cough clearance.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
期刊介绍: ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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