{"title":"原生黏液中二硫键还原诱导的宏观异质性。","authors":"Giorgia Franzino, Fabiana Tescione, Domenico Larobina","doi":"10.1021/acsabm.4c01758","DOIUrl":null,"url":null,"abstract":"<p><p>Disulfide bond reducing agents have long been used as therapeutic drugs (mucolytics) for mucus hypersecretions. Breakage of disulfide bridges is known to cause a reduction in the degree of cross-linking, making the mucus more fluid. In addition to the drop in viscoelastic properties, the disulfide breakage is also known to affect the structure of the mucus on both micro- and mesoscale. Despite this knowledge, little is known about the reorganization of the mucus at the macroscopic scale. This contribution explores the effect of the reducing agent, tris(2-carboxyethyl)phosphine hydrochloride (TCEP), on the structure of native porcine gastric mucus. After exposing the mucus to increasing concentrations of TCEP, we measure the macroscopic rheological properties along with its microscopic dynamics. The results obtained show an increase in macroscopic heterogeneity with TCEP, which we attribute to the mucus tendency to phase separate. Furthermore, we examine the soluble fractions of the different reduced mucuses by measuring the size of the suspendable macromolecules. The results indicate that the size remains approximately constant with an increasing TCEP concentration. Our contribution may be important to describe the effects of a disulfide reducing agent on the mucus structure and, consequently, on mucociliary and cough clearance.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"4647-4656"},"PeriodicalIF":4.7000,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12175130/pdf/","citationCount":"0","resultStr":"{\"title\":\"Macroheterogeneities Induced by Disulfide Bond Reduction in Native Mucus.\",\"authors\":\"Giorgia Franzino, Fabiana Tescione, Domenico Larobina\",\"doi\":\"10.1021/acsabm.4c01758\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Disulfide bond reducing agents have long been used as therapeutic drugs (mucolytics) for mucus hypersecretions. Breakage of disulfide bridges is known to cause a reduction in the degree of cross-linking, making the mucus more fluid. In addition to the drop in viscoelastic properties, the disulfide breakage is also known to affect the structure of the mucus on both micro- and mesoscale. Despite this knowledge, little is known about the reorganization of the mucus at the macroscopic scale. This contribution explores the effect of the reducing agent, tris(2-carboxyethyl)phosphine hydrochloride (TCEP), on the structure of native porcine gastric mucus. After exposing the mucus to increasing concentrations of TCEP, we measure the macroscopic rheological properties along with its microscopic dynamics. The results obtained show an increase in macroscopic heterogeneity with TCEP, which we attribute to the mucus tendency to phase separate. Furthermore, we examine the soluble fractions of the different reduced mucuses by measuring the size of the suspendable macromolecules. The results indicate that the size remains approximately constant with an increasing TCEP concentration. Our contribution may be important to describe the effects of a disulfide reducing agent on the mucus structure and, consequently, on mucociliary and cough clearance.</p>\",\"PeriodicalId\":2,\"journal\":{\"name\":\"ACS Applied Bio Materials\",\"volume\":\" \",\"pages\":\"4647-4656\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2025-06-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12175130/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Bio Materials\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1021/acsabm.4c01758\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/5/19 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1021/acsabm.4c01758","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/19 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
Macroheterogeneities Induced by Disulfide Bond Reduction in Native Mucus.
Disulfide bond reducing agents have long been used as therapeutic drugs (mucolytics) for mucus hypersecretions. Breakage of disulfide bridges is known to cause a reduction in the degree of cross-linking, making the mucus more fluid. In addition to the drop in viscoelastic properties, the disulfide breakage is also known to affect the structure of the mucus on both micro- and mesoscale. Despite this knowledge, little is known about the reorganization of the mucus at the macroscopic scale. This contribution explores the effect of the reducing agent, tris(2-carboxyethyl)phosphine hydrochloride (TCEP), on the structure of native porcine gastric mucus. After exposing the mucus to increasing concentrations of TCEP, we measure the macroscopic rheological properties along with its microscopic dynamics. The results obtained show an increase in macroscopic heterogeneity with TCEP, which we attribute to the mucus tendency to phase separate. Furthermore, we examine the soluble fractions of the different reduced mucuses by measuring the size of the suspendable macromolecules. The results indicate that the size remains approximately constant with an increasing TCEP concentration. Our contribution may be important to describe the effects of a disulfide reducing agent on the mucus structure and, consequently, on mucociliary and cough clearance.
期刊介绍:
ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications.
The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.