Easy, Rapid and High-Throughput Production of Single and Multicompartment Liposomes by Vortex Emulsification

Cell-sized liposomes, the self-assembled phospholipid vesicles with bilayer membranes, have garnered substantial attention across various fields, particularly as cell mimics. In this study, we introduce a simplified method for rapidly producing single- and multicompartment liposomes using a common laboratory vortex mixer. The simplicity of our method has the potential to greatly facilitate artificial cell and tissue-related research, potentially leading to broader applications of liposomes.