An integrated centrifugal microfluidic system for automatic nanozyme ELISA lateral flow immunoassay at point-of-care testing

The accurate and rapid detection of H1N1 viral specific protein is crucial for effective disease control and management. Traditional diagnostic methods based on ELISA immunoassay often require complex manual operations, long incubation times, and lack the integration needed for automated, high-throughput analysis. There is an urgent demand for a portable, sensitive, integrated, and fully automated diagnostic platform to address these challenges in medical diagnostics. We developed an automated diagnostic system that integrates nanozyme-based detection within a centrifugal microfluidic device for rapid H1N1 viral specific protein detection. The system utilizes nanozymes for signal amplification, leveraging nanozymes to reduce assay times. A high-resolution image sensor and image processing algorithm enable precise interrogation of test results, demonstrating significant improvements in sensitivity. The compact centrifugal microfluidic device automates the sequential delivery of samples, reagents, and nanozyme probes, seamlessly implements mixing, incubation, and liquid transfer. The system achieves a 50-fold sensitivity improvement over the conventional method based on colloidal gold lateral flow strips, with a test time of only 15 min. This work develops a fully automated, integrated diagnostic platform by combining nanozyme-enhanced immunoassay with automatic centrifugal microfluidics. The platform eliminates manual intervention, achieving high sensitivity and rapid detection of infectious disease viral specific protein. Its compact and integrated design makes it a versatile tool for point-of-care diagnostics.