转录组学揭示低温条件下中国鳖卵巢发育的肌酸调节机制

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-05-20 DOI:10.1016/j.aqrep.2025.102867

Guoyin Zhang , Xi He , Yulong Li , Jiazhen Wu , Guoying Qian , Zhongfa Chen , Caiyan Li , Wei Wang

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引用次数: 0

摘要

一些经济上重要的动物冬眠；然而，它们在低温禁食期间的能量调节仍然知之甚少。为了研究中国鳖（Pelodiscus sinensis）卵巢在低温条件下的发育情况，采用浓度为50 ~ 400 mg/kg体重的肌酸对早熟鳖进行处理。分析卵巢卵泡发育、肌酸激酶（CK）表达和肝脏转录组学。肌酸处理8周后，卵巢卵泡直径和蛋黄颗粒积累显著增加（P <； 0.05），CK表达显著上调（P <； 0.05），卵巢乳酸水平显著降低（P <； 0.05）。转录组分析在肝脏中鉴定出4355个差异表达基因。肌酸处理显著增加了VTG、LEPR、LHR、FSHR和CK-B基因的表达，脂肪细胞因子信号通路、氧化磷酸化、促性腺激素释放激素（GnRH）信号通路和过氧化物酶体增殖物激活受体信号通路中的基因显著上调。肌酸还能增强脂肪细胞的瘦素分泌，通过GnRH信号通路促进卵巢发育。本研究为了解中华按蚊的能量代谢奠定了基础，为改善低温条件下冬眠动物的能量管理和生殖潜力提供了参考。

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Transcriptomics reveals creatine regulation of ovarian development at low temperature in Chinese soft-shelled turtles (Pelodiscus sinensis)

Some economically important animals hibernate; however, their energy regulation during low-temperature fasting remain poorly understood. To investigate ovarian development in Chinese soft-shelled turtles (Pelodiscus sinensis) under low-temperature conditions, premature turtles were treated with creatine at concentrations of 50–400 mg/kg body weight. Ovarian follicle development, creatine kinase (CK) expression, and liver transcriptomics were analyzed. After 8 weeks of creatine treatment, ovarian follicle diameters and yolk granule accumulation increased significantly (P < 0.05), CK expression was significantly upregulated (P < 0.05), and ovarian lactic acid levels decreased significantly (P < 0.05). Transcriptome analysis identified 4355 differentially expressed genes in the liver. Creatine treatment significantly increased the expression of VTG, LEPR, LHR, FSHR, and CK-B genes, and genes in the adipocytokine signaling, oxidative phosphorylation, gonadotropin-releasing hormone (GnRH) signaling, and peroxisome proliferator–activated receptor signaling pathways were significantly upregulated. Creatine administration also enhanced leptin secretion from adipocytes, promoting ovarian development via the GnRH signaling pathway. This study provides a foundation for understanding energy metabolism in P. sinensis, offering insights to improve energy management and reproductive potential in hibernating animals under low-temperature conditions.

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.