设计一个Mi3Chemout系统，用于控制感兴趣的蛋白质的招募和释放。

IF 4.3 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Communications Pub Date : 2025-05-13 DOI:10.1039/d5cc00808e

Xiao Ma , Chunxue Liu , Lingqin Wang , Shijie Ren , Yongsheng Yu , Hong Yuan , Wei Kang , Chuang Xue

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引用次数: 0

摘要

蛋白笼是提呈感兴趣蛋白（POI）的理想支架，但控制POI的释放仍然具有挑战性。我们设计了具有化学诱导二聚化结构域和光可切割蛋白的Mi3蛋白笼，使poi在体外和大肠杆菌细胞中均可控制募集和释放。

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Engineering a Mi3Chemout system for controlled recruitment and release of proteins of interest†

Protein cages are ideal scaffolds for presenting proteins of interest (POIs), but controlled POI release remains challenging. We engineered the Mi3 protein cage with chemically inducible dimerization domains and a photocleavable protein, enabling controlled recruitment and release of POIs both in vitro and in E. coli cells.

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来源期刊

Chemical Communications 化学-化学综合

CiteScore

8.60

自引率

4.10%

发文量

2705

审稿时长

1.4 months

期刊介绍： ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.