{"title":"利用双足dna - walker介导的BiOI/ in2o3门控有机光电化学晶体管生物传感器双模式检测RNA去甲基化蛋白ALKBH3。","authors":"Yixin Hu, Yunlei Zhou, Huanshun Yin, Zhidong Yin, Tengjin Wang, Xingyu Liu, Xueyan Li, Zhe Chi","doi":"10.1002/smll.202502160","DOIUrl":null,"url":null,"abstract":"<p>Organic photoelectrochemical transistor (OPECT) biosensors have emerged as prevalent candidates for bioanalytical technologies because of their intrinsic amplification properties and excellent detection performance. Herein, a novel OPECT biosensor for analyzing the RNA demethylation protein ALKBH3 is developed. A BiOI/In<sub>2</sub>O<sub>3</sub> type-II heterojunction is employed as the photoactive material for the gate, and an organic semiconductor, poly(3,4-ethylenedioxythiophene): poly(styrenesulfonic) (PEDOT:PSS), is used as the channel modification material. ALKBH3-mediated RNA demethylation, as a switch, triggered the trans-cleavage activity of the CRISPR-Cas12a system, which subsequently leads to a failure in the formation of a bipedal DNA walker. Contrarily, ferrocenecarboxylic acid (FcA), which acts as an effective electron donor, cannot be captured, leading to modulation of the gating characteristics and degradation of the channel current response. Compared to the conventional photoelectrochemical method, the OPECT biosensor exhibits higher sensitivity and a lower detection limit for ALKBH3 protein, thereby facilitating the monitoring of ALKBH3 protein activity changes under the effect of environmental pollutants and demonstrating the crucial biomarker potential of ALKBH3 protein in evaluating its toxicological effects.</p>","PeriodicalId":228,"journal":{"name":"Small","volume":"21 27","pages":""},"PeriodicalIF":12.1000,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dual-Mode Detection of RNA Demethylation Protein ALKBH3 Using a Two-Legged DNA-Walker-Mediated BiOI/In2O3-Gated Organic Photoelectrochemical Transistor Biosensor\",\"authors\":\"Yixin Hu, Yunlei Zhou, Huanshun Yin, Zhidong Yin, Tengjin Wang, Xingyu Liu, Xueyan Li, Zhe Chi\",\"doi\":\"10.1002/smll.202502160\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Organic photoelectrochemical transistor (OPECT) biosensors have emerged as prevalent candidates for bioanalytical technologies because of their intrinsic amplification properties and excellent detection performance. Herein, a novel OPECT biosensor for analyzing the RNA demethylation protein ALKBH3 is developed. A BiOI/In<sub>2</sub>O<sub>3</sub> type-II heterojunction is employed as the photoactive material for the gate, and an organic semiconductor, poly(3,4-ethylenedioxythiophene): poly(styrenesulfonic) (PEDOT:PSS), is used as the channel modification material. ALKBH3-mediated RNA demethylation, as a switch, triggered the trans-cleavage activity of the CRISPR-Cas12a system, which subsequently leads to a failure in the formation of a bipedal DNA walker. Contrarily, ferrocenecarboxylic acid (FcA), which acts as an effective electron donor, cannot be captured, leading to modulation of the gating characteristics and degradation of the channel current response. Compared to the conventional photoelectrochemical method, the OPECT biosensor exhibits higher sensitivity and a lower detection limit for ALKBH3 protein, thereby facilitating the monitoring of ALKBH3 protein activity changes under the effect of environmental pollutants and demonstrating the crucial biomarker potential of ALKBH3 protein in evaluating its toxicological effects.</p>\",\"PeriodicalId\":228,\"journal\":{\"name\":\"Small\",\"volume\":\"21 27\",\"pages\":\"\"},\"PeriodicalIF\":12.1000,\"publicationDate\":\"2025-05-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Small\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/smll.202502160\",\"RegionNum\":2,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Small","FirstCategoryId":"88","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/smll.202502160","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Dual-Mode Detection of RNA Demethylation Protein ALKBH3 Using a Two-Legged DNA-Walker-Mediated BiOI/In2O3-Gated Organic Photoelectrochemical Transistor Biosensor
Organic photoelectrochemical transistor (OPECT) biosensors have emerged as prevalent candidates for bioanalytical technologies because of their intrinsic amplification properties and excellent detection performance. Herein, a novel OPECT biosensor for analyzing the RNA demethylation protein ALKBH3 is developed. A BiOI/In2O3 type-II heterojunction is employed as the photoactive material for the gate, and an organic semiconductor, poly(3,4-ethylenedioxythiophene): poly(styrenesulfonic) (PEDOT:PSS), is used as the channel modification material. ALKBH3-mediated RNA demethylation, as a switch, triggered the trans-cleavage activity of the CRISPR-Cas12a system, which subsequently leads to a failure in the formation of a bipedal DNA walker. Contrarily, ferrocenecarboxylic acid (FcA), which acts as an effective electron donor, cannot be captured, leading to modulation of the gating characteristics and degradation of the channel current response. Compared to the conventional photoelectrochemical method, the OPECT biosensor exhibits higher sensitivity and a lower detection limit for ALKBH3 protein, thereby facilitating the monitoring of ALKBH3 protein activity changes under the effect of environmental pollutants and demonstrating the crucial biomarker potential of ALKBH3 protein in evaluating its toxicological effects.
期刊介绍:
Small serves as an exceptional platform for both experimental and theoretical studies in fundamental and applied interdisciplinary research at the nano- and microscale. The journal offers a compelling mix of peer-reviewed Research Articles, Reviews, Perspectives, and Comments.
With a remarkable 2022 Journal Impact Factor of 13.3 (Journal Citation Reports from Clarivate Analytics, 2023), Small remains among the top multidisciplinary journals, covering a wide range of topics at the interface of materials science, chemistry, physics, engineering, medicine, and biology.
Small's readership includes biochemists, biologists, biomedical scientists, chemists, engineers, information technologists, materials scientists, physicists, and theoreticians alike.