利用双足dna - walker介导的BiOI/ in2o3门控有机光电化学晶体管生物传感器双模式检测RNA去甲基化蛋白ALKBH3。

IF 12.1 2区 材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Small Pub Date : 2025-05-19 DOI:10.1002/smll.202502160
Yixin Hu, Yunlei Zhou, Huanshun Yin, Zhidong Yin, Tengjin Wang, Xingyu Liu, Xueyan Li, Zhe Chi
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引用次数: 0

摘要

有机光电化学晶体管(OPECT)生物传感器因其固有的放大特性和优异的检测性能而成为生物分析技术的热门候选。本文开发了一种用于分析RNA去甲基化蛋白ALKBH3的新型OPECT生物传感器。采用BiOI/In2O3型异质结作为栅的光活性材料,采用有机半导体聚(3,4-乙烯二氧噻吩):聚(苯乙烯磺酸)(PEDOT:PSS)作为通道修饰材料。alkbh3介导的RNA去甲基化,作为一个开关,触发了CRISPR-Cas12a系统的反式切割活性,随后导致双足DNA行走器形成失败。相反,二茂铁羧酸(FcA)作为一个有效的电子供体,不能被捕获,导致门控特性的调制和通道电流响应的退化。与传统的光电化学方法相比,OPECT生物传感器对ALKBH3蛋白具有更高的灵敏度和更低的检出限,从而便于监测环境污染物作用下ALKBH3蛋白活性的变化,展示了ALKBH3蛋白在评价其毒理学效应方面的重要生物标志物潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Dual-Mode Detection of RNA Demethylation Protein ALKBH3 Using a Two-Legged DNA-Walker-Mediated BiOI/In2O3-Gated Organic Photoelectrochemical Transistor Biosensor

Dual-Mode Detection of RNA Demethylation Protein ALKBH3 Using a Two-Legged DNA-Walker-Mediated BiOI/In2O3-Gated Organic Photoelectrochemical Transistor Biosensor

Organic photoelectrochemical transistor (OPECT) biosensors have emerged as prevalent candidates for bioanalytical technologies because of their intrinsic amplification properties and excellent detection performance. Herein, a novel OPECT biosensor for analyzing the RNA demethylation protein ALKBH3 is developed. A BiOI/In2O3 type-II heterojunction is employed as the photoactive material for the gate, and an organic semiconductor, poly(3,4-ethylenedioxythiophene): poly(styrenesulfonic) (PEDOT:PSS), is used as the channel modification material. ALKBH3-mediated RNA demethylation, as a switch, triggered the trans-cleavage activity of the CRISPR-Cas12a system, which subsequently leads to a failure in the formation of a bipedal DNA walker. Contrarily, ferrocenecarboxylic acid (FcA), which acts as an effective electron donor, cannot be captured, leading to modulation of the gating characteristics and degradation of the channel current response. Compared to the conventional photoelectrochemical method, the OPECT biosensor exhibits higher sensitivity and a lower detection limit for ALKBH3 protein, thereby facilitating the monitoring of ALKBH3 protein activity changes under the effect of environmental pollutants and demonstrating the crucial biomarker potential of ALKBH3 protein in evaluating its toxicological effects.

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来源期刊
Small
Small 工程技术-材料科学:综合
CiteScore
17.70
自引率
3.80%
发文量
1830
审稿时长
2.1 months
期刊介绍: Small serves as an exceptional platform for both experimental and theoretical studies in fundamental and applied interdisciplinary research at the nano- and microscale. The journal offers a compelling mix of peer-reviewed Research Articles, Reviews, Perspectives, and Comments. With a remarkable 2022 Journal Impact Factor of 13.3 (Journal Citation Reports from Clarivate Analytics, 2023), Small remains among the top multidisciplinary journals, covering a wide range of topics at the interface of materials science, chemistry, physics, engineering, medicine, and biology. Small's readership includes biochemists, biologists, biomedical scientists, chemists, engineers, information technologists, materials scientists, physicists, and theoreticians alike.
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