从人参皂苷Rg1中提取的碳纳米点对宫颈癌有很好的抑制作用

IF 5.5 3区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY
Jiahui Bao, Xuechun Zhou, Xiaoyu He, Ning Tian, Zhe Zhang, Hui Peng, Changkui Fu, Tenghui Tian, Yu Zhao, Yingnan Jiang, Rui Jiang, Andrew K. Whittaker
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引用次数: 0

摘要

目前宫颈癌患者的标准治疗方案包括放疗和化疗相结合。然而,化疗药物经常遇到的严重副作用极大地限制了有效剂量,因此宫颈癌的治疗仍然面临着巨大的挑战。本研究以人参皂苷Rg1为原料制备了具有抗宫颈癌活性且毒性可忽略不计的纳米药物——碳纳米点。Rg1碳纳米点表面含有丰富的亲水性官能团,在水介质中分散性好,生物相容性高。体外实验表明,Rg1碳纳米点对HeLa细胞具有显著的细胞抑制和促凋亡作用,并能抑制HeLa细胞的迁移和侵袭。荷瘤裸鼠实验表明,Rg1碳纳米点能显著抑制肿瘤生长。通过qPCR验证,Rg1碳纳米点可通过调节Cyto c、Caspase-9、Caspase-3、Bax和Bcl-2的表达水平促进HeLa细胞凋亡,通过调节cdk1和Cyclin B1的表达诱导G2/M期阻滞,通过调节CDH1和β-catenin抑制肿瘤细胞迁移。由于前体Rg1是天然草药提取物,因此在裸鼠中观察到的毒副作用可以忽略不计。研究表明,Rg1碳纳米点有望成为一种潜在的治疗人类宫颈癌的纳米药物,毒副作用可以忽略不计,治疗效果极佳。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Carbon nanodots derived from herbal extract ginsenoside Rg1 demonstrate highly effective inhibition against cervical carcinoma

The current standard treatment regimen for patients with cervical cancer consists of a combination of radiotherapy and chemotherapy. However, the serious side effects often encountered with chemotherapy drugs greatly limits the effective doses that can be delivered, and hence the treatment of cervical cancer still faces strong challenges. In this study, carbon nanodots, nanodrugs with anti-cervical cancer activity and with negligible toxicity, were prepared from the precursor herbal extract ginsenoside Rg1. The surface of the Rg1 carbon nanodots is rich in hydrophilic functional groups, resulting in good dispersion in aqueous media and high biocompatibility. In Vitro experiments show that the Rg1 carbon nanodots have significant cytostatic and pro-apoptotic effects on HeLa cells, and could inhibit their migration and invasion. Experiments in tumor-bearing nude mice show that the Rg1 carbon nanodots could significantly inhibit tumor growth. Through qPCR validation, the Rg1 carbon nanodots were shown to enhance HeLa cell apoptosis, by regulating the expression levels of Cyto c, Caspase-9, Caspase-3, Bax, and Bcl-2, induce G2/M phase arrest by regulating CDK 1 and Cyclin B1 expression, and inhibit tumor cell migration by modulating CDH1 and β-catenin. Since the precursor Rg1 is a natural herbal extract, negligible toxic side effects were observed in nude mice. The work demonstrates that Rg1 carbon nanodots can be expected to become a potential nanomedicine against human cervical cancer with negligible toxic side effects and excellent therapeutic effects.

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来源期刊
Carbon Letters
Carbon Letters CHEMISTRY, MULTIDISCIPLINARY-MATERIALS SCIENCE, MULTIDISCIPLINARY
CiteScore
7.30
自引率
20.00%
发文量
118
期刊介绍: Carbon Letters aims to be a comprehensive journal with complete coverage of carbon materials and carbon-rich molecules. These materials range from, but are not limited to, diamond and graphite through chars, semicokes, mesophase substances, carbon fibers, carbon nanotubes, graphenes, carbon blacks, activated carbons, pyrolytic carbons, glass-like carbons, etc. Papers on the secondary production of new carbon and composite materials from the above mentioned various carbons are within the scope of the journal. Papers on organic substances, including coals, will be considered only if the research has close relation to the resulting carbon materials. Carbon Letters also seeks to keep abreast of new developments in their specialist fields and to unite in finding alternative energy solutions to current issues such as the greenhouse effect and the depletion of the ozone layer. The renewable energy basics, energy storage and conversion, solar energy, wind energy, water energy, nuclear energy, biomass energy, hydrogen production technology, and other clean energy technologies are also within the scope of the journal. Carbon Letters invites original reports of fundamental research in all branches of the theory and practice of carbon science and technology.
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