马来西亚入侵小龙虾四爪螯虾（Cherax quadricarinatus）相关Temnocephalids的遗传和种群多样性

IF 1.2 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2025-05-18 DOI:10.1007/s11686-025-01050-x

Norhan N. Azri-Shah, Nor Asma Husna Yusoff, Farizan Abdullah, Ahmad Najmi Ishak, Norainy Mohd Husin, Hin Boo Wee, John Brian Jones, Muhammad Ikhwanuddin, Siti Azizah Mohd Nor, Marina Hassan

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引用次数: 0

摘要

目的通过对马来西亚马六甲和柔佛州的研究，发现在马六甲和柔佛州，斑尾目（temnocephalids Diceratocephala boschmai）、爪尾目（Craspedella pedum）和足尾目（Decadidymus sp.）在斑尾目（Cheraq quadricarinatus）中高度流行。方法采用28S核糖体DNA和线粒体细胞色素c氧化酶亚基I （CO1）两种DNA标记对四叉棘鱼的temnocephalids进行分子鉴定。随后，利用CO1 DNA标记分析了马来西亚boschmai的种群多样性，以可视化种群间的遗传关联。结果对博施麦D. boschmai CO1基因序列的16个可变（分离）位点进行分析，结果显示群体间单倍型多样性为0.1266，表明群体内存在遗传变异。此外，核苷酸多样性（II）的测定结果为0.0035，Tajima的D中性检验结果为负值，表明所有种群都没有明显偏离中性进化。结论amova分析表明，遗传变异或多态性主要来自群体内（95%），而群体间（4%）。

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Genetic and Population Diversity of Temnocephalids Associated with the Invasive Crayfish Cherax quadricarinatus in Malaysia

Purpose

A previous study revealed that temnocephalids Diceratocephala boschmai, Craspedella pedum, and Decadidymus sp. were highly prevalent in Cheraq quadricarinatus in Melaka and Johor, Malaysia.

Methods

Herein, study was conducted to extract temnocephalids from C. quadricarinatus and identified using a molecular approach via two DNA markers: 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I (CO1). Thereafter, the population diversity of D. boschmai in Malaysia using the CO1 DNA marker was analysed to visualize the genetic associations among populations.

Results

The analysis of 16 variable (segregating) sites in the CO1 gene alignment of sequence from D. boschmai revealed a calculated haplotype diversity of 0.1266 among populations which indicates genetic variation within the studied groups. Additionally, the determination of nucleotide diversity (II) yielded a value of 0.0035, and Tajima's D neutrality test yielded a negative value for all populations, suggesting no significant departure from neutral evolution.

Conclusion

AMOVA analysis indicated that the genetic variation or polymorphism is primarily contributed within populations (95%) compared to between populations (4%).

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.